A5077231671- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Escherichia coli research studies
- Microtubule and mitosis dynamics
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Micro and Nano Robotics
- Glycosylation and Glycoproteins Research
- Hemoglobin structure and function
- Vibrio bacteria research studies
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Erythrocyte Function and Pathophysiology
- Legume Nitrogen Fixing Symbiosis
- Bacterial biofilms and quorum sensing
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Enzyme Structure and Function
- Salmonella and Campylobacter epidemiology
- Enzyme Production and Characterization
- Microfluidic and Bio-sensing Technologies
- Cancer-related Molecular Pathways
Prefectural University of Hiroshima
2013-2024
Tohoku University
2011
Japan Science and Technology Agency
2004-2008
Soft99 (Japan)
2006-2008
University of Nottingham
2007
Queen's Medical Centre
2007
Aoyama Gakuin University
2006
Teikyo University
1994-2005
University of Utah
2005
University of Hawaiʻi at Mānoa
2004
The type III secretion system of Salmonella typhimurium directs the translocation proteins into host cells. Evolutionarily related to flagellar assembly machinery, this is also present in other pathogenic bacteria, but its organization unknown. Electron microscopy revealed supramolecular structures spanning inner and outer membranes flagellated nonflagellated strains; such were not detected strains carrying null mutations components apparatus. Isolated found contain at least three system....
Many bacterial pathogens of plants and animals have evolved a specialized protein-secretion system termed type III to deliver proteins into host cells. These stimulate or interfere with cellular functions for the pathogen's benefit. The Salmonella typhimurium pathogenicity island 1 encodes one these systems that mediates this bacterium's ability enter nonphagocytic Several components secretion are organized in supramolecular structure needle complex. This is made discrete substructures...
The length of flagellar hooks isolated from wild-type and mutant cells with various hook lengths were measured on electron micrographs. the showed a narrow distribution peak (+/- standard deviation) at 55.0 +/- 5.9 nm, whereas fliK mutants (so-called polyhook mutants) broad ranging 40 to 900 strongly indicating that FliK is involved in determination. Among pseudorevertants such mutants, intragenic suppressors gave rise filaments. However, intergenic mapping flhB also abnormal length, albeit...
Three flagellar genes of Salmonella typhimurium (flaAII.2, flaQ, and flaN) were found to be multifunctional, each being associated with four distinct mutant phenotypes: nonflagellate (Fla-), paralyzed (Mot-), nonchemotactic (Che-) clockwise motor bias, counterclockwise bias. The distribution Fla, Mot, Che mutational sites within gene was examined. Fla fairly broadly distributed, whereas Mot more narrowly defined. Local subregions rich in one type not generally another type. Among sites,...
Mutations in the fliK gene of Salmonella typhimurium commonly cause failure to terminate hook assembly and initiate filament (polyhook phenotype). Polyhook mutants give rise pseudorevertants which are still defective termination but have recovered ability assemble (polyhook-filament The polyhook mutations been found be either frameshift or nonsense, resulting truncation C terminus FliK. Intragenic suppressors were ones that restored original frame (and therefore C-terminal sequence), most...
The hook-basal body complex of Salmonella typhimurium, a major component its flagellar apparatus, was subjected to detailed analysis by electron microscopy and gel electrophoresis. study facilitated the development an improved protocol for isolation complexes in high yield purity. Nine proteins were identified with structure. These had apparent molecular weights 65,000 (65K), 60K, 42K, 38K, 32K, 30K, 27K, 16K, 14K. Small but reproducible shifts specific from conditionally nonflagellate...
The flhDC operon of Salmonella typhimurium is the master control required for expression entire flagellar regulon. was placed under tetracycline‐inducible promoter P tetA using T‐POP transposon. Cells containing this construct are motile in presence tetracycline and non‐motile without inducer present. No flagella were visible electron microscope when cells grown inducer. class 1, 2 3 promoters regulon temporally regulated. After addition tetracycline, 1 transcribed immediately. Transcription...
Abstract Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (TTSS) to inject effector proteins into the plasma membrane and cytosol of infected cells. To translocate proteins, EPEC, like Salmonella Shigella, is believed assemble macromolecular complex (type secreton) that spans both bacterial membranes has short needle-like projection. However, there special interest in studying EPEC TTSS owing fact one secreted EspA, assembled unique filamentous structure also...
In wild‐type Salmonella , the length of flagellar hook, a structure consisting subunits hook protein FlgE, is fairly tightly controlled at ≈ 55 nm. Because fliK mutants produce abnormally elongated structures that lack filament structure, FliK appears to be involved in both termination elongation and initiation formation. FliK, soluble protein, believed function together with membrane FlhB, export apparatus mediate switching substrate specificity (from flagellin) upon completion assembly. We...
In Xenopus oocytes, Mos activates the mitogen-activated protein kinase (MAPK) signal transduction cascade and regulates meiosis. mammalian however, functions of are still unclear. present study, we used c-mos knockout mouse oocytes examined roles in oocyte maturation fertilization, including whether controls MAPK promoting factor (MPF) activity. The kinetics germinal vesicle breakdown (GVBD) first polar body emission were similar wild-type, heterozygous mutant, homozygous mutant mice....
We analysed all major proteins secreted into culture media from Salmonella typhimurium . Proteins in supernatants were collected by trichloroacetic acid precipitation, separated SDS–polyacrylamide gels and amino sequencing. Wild‐type strain SJW1103 cells typically gave rise to nine bands SDS gels: 89, 67, 58, 52, 50, 42, 40, 35 (sometimes) 28 kDa. A search of the sequences available databases revealed that they either flagellar or virulence factors. Six them flagella specific: FlgK HAP1 (58...
The predatory bacterium Bdellovibrio bacteriovorus swims rapidly by rotation of a single, polar flagellum comprised helical filament flagellin monomers, contained within membrane sheath and powered basal motor complex. collides with, enters replicates bacterial prey, process previously suggested to firstly require flagellar motility then shedding upon prey entry. Here we show that flagella are not always shed entry study the six fliC genes B. bacteriovorus, finding them all conserved...
We report the complete genome sequence of deep-sea γ-proteobacterium, Idiomarina loihiensis , isolated recently from a hydrothermal vent at 1,300-m depth on Lōihi submarine volcano, Hawaii. The I. comprises single chromosome 2,839,318 base pairs, encoding 2,640 proteins, four rRNA operons, and 56 tRNA genes. A comparison to genomes other γ-proteobacteria reveals abundance amino acid transport degradation enzymes, but loss sugar systems certain enzymes metabolism. This finding suggests that...
Summary The archaeal flagellum is a unique motility apparatus in the prokaryotic domain, distinct from bacterial flagellum. Most of currently recognized flagella‐associated genes fall into single fla operon that contains for flagellin proteins (two or more designated as flaA flaB ), some variation set conserved unknown function ( flaC , flaD flaE flaF flaG and flaH an ATPase flaI ) membrane protein flaJ ). In addition, gene has been demonstrated to encode two proteins: full‐length product...
Bdellovibrio bacteriovorus is a Delta-proteobacterium that oscillates between free-living growth and predation on Gram-negative bacteria including important pathogens of man, animals plants. After entering the prey periplasm, killing replicating inside bdelloplast, several motile B. progeny cells emerge. The HD100 genome encodes numerous proteins predicted to be involved in signalling via secondary messenger cyclic di-GMP (c-di-GMP), which known affect bacterial lifestyle choices. We...
Bdellovibrio are predatory bacteria that have evolved to invade virtually all Gram-negative bacteria, including many prominent pathogens. Upon invasion, prey become rounded up into an osmotically stable niche for the Bdellovibrio, preventing further superinfection and allowing replicate inside without competition, killing bacterium degrading its contents. Historically, rounding was hypothesized be associated with peptidoglycan (PG) metabolism; we found two genes, bd0816 bd3459, expressed at...