A5103939352- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Viral gastroenteritis research and epidemiology
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Viral Infectious Diseases and Gene Expression in Insects
- Parasitic Infections and Diagnostics
- Machine Learning in Bioinformatics
- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Cancer-related molecular mechanisms research
- Trypanosoma species research and implications
- Pancreatic function and diabetes
- RNA modifications and cancer
- FOXO transcription factor regulation
- Clostridium difficile and Clostridium perfringens research
- Genetics, Bioinformatics, and Biomedical Research
National Cancer Institute
2014
Washington University in St. Louis
2003-2013
Memorial Sloan Kettering Cancer Center
2011
Imperial College London
2001-2007
Hebrew University of Jerusalem
2007
Wokingham Hospital
2003
Electrosciences (United Kingdom)
2002
University of Oklahoma
1983-1989
The complete sequence of the 17,553-nucleotide Xenopus laevis mitochondrial genome has been determined. A comparison this amphibian genomic with those mammalian genomes reveals a similar gene order and compact organization. encoded genes for 22 tRNAs, two ribosomal RNAs, 13 proteins (COI, COII, COIII, ATPase 6, cytochrome b, eight additional unidentified reading frames) in mitochondria are highly homologous to their counterparts. Although contains significantly larger displacement loop...
To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract separated by two-dimensional electrophoresis. The visualized spots were identified through combination peptide sequencing and reverse genetic methodologies. Of 30 as S. specific, 27 have been identified. Six these proteins, previously unidentified in agalactiae, sequenced cloned. These ornithine carbamoyltransferase, phosphoglycerate kinase,...
Abstract Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (TTSS) to inject effector proteins into the plasma membrane and cytosol of infected cells. To translocate proteins, EPEC, like Salmonella Shigella, is believed assemble macromolecular complex (type secreton) that spans both bacterial membranes has short needle-like projection. However, there special interest in studying EPEC TTSS owing fact one secreted EspA, assembled unique filamentous structure also...
ABSTRACT Citrobacter rodentium is a member of group pathogens that colonize the lumen host gastrointestinal tract via attaching and effacing (A/E) lesion formation. C. , which causes transmissible colonic hyperplasia in mice, used as an vivo model system for clinically significant A/E enterohemorrhagic enteropathogenic Escherichia coli . These bacteria all contain pathogenicity island called locus enterocyte effacement (LEE), encodes type III secretion designed to deliver effector proteins...
Type III secretion systems, designed to deliver effector proteins across the bacterial cell envelope and plasma membrane of target eukaryotic cell, are involved in subversion functions a variety human, animal plant pathogens. In enteropathogenic Escherichia coli (EPEC), several protein substrates for apparatus were identified, including EspA, EspB EspD. EspA is structural major component large transiently expressed filamentous surface organelle that forms direct link between bacterium host...
Summary Citrobacter rodentium is used as an in vivo model system for clinically significant enteric pathogens such enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. (EPEC). These all colonize the lumen side of host gastrointestinal tract via attaching effacing (A/E) lesion formation. In order to identify genes required colonization A/E‐forming pathogens, a library signature‐tagged transposon mutants C. was constructed screened mice. Of 576 tested, 14 were attenuated their...
We have determined the primary structure of two regions Xenopus laevis mitochondrial genome which encompass origins heavy (H) and light (L) strand replication. The first segment, consists 2398 nucleotides, contains displacement loop (D-loop), tRNA genes for threonine, proline phenylalanine, origin H-strand replication, promoters H- L-strand transcription. second 447 replication flanked by tryptophan, alanine, asparagine, cysteine, tyrosine. A comparison sequences region eight with their...
The overall structure of transfer RNA is optimized for its various functions by a series unique post-transcriptional nucleotide modifications. Since many these modifications are conserved from prokaryotes through higher eukaryotes, it has been proposed that most modified nucleotides serve to optimize the ability tRNA accurately interact with other components protein synthesizing machinery. When cloned synthetic Escherichia coli tRNAPhe gene was transfected into bacterial host carried...
Summary The type III secretion system (TTSS) is a macromolecular structure that spans the cell wall of Gram‐negative bacterial pathogens, enabling delivery virulence effector proteins directly to membranes and cytosol host eukaryotic cells. TTSS consists conserved needle complex (NC) composed sets inner outer rings connected by periplasmic rod. Enteropathogenic Escherichia coli (EPEC) an extracellular diarrhoeagenic pathogen uses induce actin polymerization colonizes intestinal epithelium....
The polydnavirus Toxoneuron nigriceps bracovirus (TnBV) is an obligate symbiont associated with the braconid wasp T. nigriceps, a parasitoid of Heliothis virescens larvae. Previously, to identify genes that allow parasitization by altering host immune and endocrine systems, expression patterns TnBV from parasitized H. larvae were analysed cDNAs obtained. To study function protein one such cDNA, TnBV1, overexpression was attempted using baculovirus Autographa californica multicapsid...
Abstract As important vectors of human disease, phlebotomine sand flies are global significance to health, transmitting several emerging and re‐emerging infectious diseases. The most devastating the fly transmitted infections leishmaniases, causing significant mortality morbidity in both O ld N ew W orld. Here we present first transcriptome analysis orld vector cutaneous leishmaniasis, P hlebotomus papatasi ( S copoli) compare this that visceral L utzomyia longipalpis . A normalized cDNA...
Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface Group B streptococcus (Streptococcus agalactiae). In enzyme-linked immunosorbent assay, antisera raised against two proteins, streptococcal yutD homologue and subunit ABC transporter, recognised clinically important serotypes streptococcus. neonatal rat model, purified IgG from sera conferred significant levels protection lethal challenge...