A5006931400- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Insect Resistance and Genetics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Animal Genetics and Reproduction
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- DNA and Nucleic Acid Chemistry
- Heat shock proteins research
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Fungal and yeast genetics research
- Carcinogens and Genotoxicity Assessment
- Plant Reproductive Biology
- Plant Virus Research Studies
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Plant Disease Resistance and Genetics
Istituto Pasteur
2005-2022
Sapienza University of Rome
2004-2022
Cold Spring Harbor Laboratory
2010
University of Kentucky
2005
National Institutes of Health
2005
National Cancer Institute
2005
University of Perugia
1994-1998
Inserm
1998
Centre National de la Recherche Scientifique
1998
University of California, Berkeley
1998
We determined the distribution of 11 different transposable elements on Drosophila melanogaster mitotic chromosomes by using high-resolution fluorescent in situ hybridization (FISH) coupled with charge-coupled device camera analysis. Nine these (copia, gypsy, mdg-1, blood, Doc, I, F, G, and Bari-1) are preferentially clustered into one or more discrete heterochromatic regions Oregon-R laboratory stock. Moreover, FISH analysis geographically distant strains revealed that locations element...
Tri-methylated lysine 20 on histone H4 (Me(3)K20H4) is a marker of constitutive heterochromatin in murine interphase and metaphase cells. Heterochromatin marked by Me(3)K20H4 replicates late during S phase the cell cycle. Serum starvation increases number cells that exhibit high levels at heterochromatin. also present centromeric most meiotic chromosomes spermatogenesis pseudoautosomal region, as well some telomeres. It not XY-body. During embryogenesis maternal pronucleus contains...
Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomal protein, which involved in heterochromatin formation and gene silencing many organisms. In addition, it has been shown that HP1 also telomere capping Drosophila. Here, we show novel striking feature of this demonstrating its involvement the activation several euchromatic genes By immunostaining experiments using an antibody, found associated with developmental heat shock–induced puffs on polytene chromosomes. Because are...
Heterochromatin Protein 1 (HP1a) is a well-known conserved protein involved in heterochromatin formation and gene silencing different species including humans. A general model has been proposed for epigenetic that implies an essential role HP1a. According to the model, histone methyltransferase enzymes (HMTases) methylate H3 at lysine 9 (H3K9me), creating selective binding sites itself chromodomain of This complex thought form higher order chromatin state represses activity. It also found...
Abstract The sting mutation, caused by a P element inserted into polytene region 32D, was isolated screen for male sterile insertions in Drosophila melanogaster. This sterility is correlated with the presence of crystals spermatocytes and spermatids that are structurally indistinguishable from those produced males carrying deficiency Y-linked crystal (cry) locus. In addition, their morphology needle-like Ste+ flies star-shaped Ste flies, once again as observed cry– males. sti mutation leads...
Heterochromatin protein 1 (HP1), a gene silencing protein, localizes to centric heterochromatin through an interaction with methylated K9 of histone H3, modification generated by the methyl transferase SU(VAR)3-9. On Drosophila polytene chromosomes, HP1 also 200 sites scattered throughout euchromatin. To address role in euchromatic regulation, mRNAs from wild-type and Su(var)2-5 mutants lacking were compared. Genes residing within 550-kb genomic region enriched that show altered expression...
The distinct structural properties of heterochromatin accommodate a diverse group vital chromosome functions, yet we have only rudimentary molecular details its structure. A powerful tool in the analyses structure Drosophila has been mutations that reverse repressive effect on expression gene placed next to it ectopically. Several genes from this are known encode proteins enriched heterochromatin. best characterized these is heterochromatin-associated protein, HP1. HP1 no DNA-binding...
We have identified a new middle repetitive DNA family in Drosophila melanogaster. This is composed of 1.7-kb element, called Bari-1, that shows common characteristics with many transposable elements. Bari-1 present few euchromatic sites vary different stocks. However, it peculiar most copies are homogeneously clustered unique location specific heterochromatic region close to the centromere second chromosome. The molecular analysis coming from euchromatin and heterochromatin has revealed...
The segregation distortion phenomenon occurs in Drosophila melanogaster males carrying an SD second chromosome and SD+ homolog. In such the is transmitted to progeny more frequently than expected 50% because of abnormal differentiation SD+-bearing sperms. Three major loci are involved this phenomenon: Rsp, associated with chromosome, respectively, E(SD). present work we performed a cytogenetic analysis Rsp locus which was known map centromeric heterochromatin chromosome. Hoechst- N-banding...
Previous studies have shown that heat shock stress may activate transposable elements (TEs) in Drosophila and other organisms. Such an effect depends on the disruption of a chaperone complex is normally involved biogenesis Piwi-interacting RNAs (piRNAs), largest class germline-enriched small noncoding implicated epigenetic silencing TEs. However, satisfying picture how chaperones could be repressing TEs germ cells still unknown. Here we show that, Drosophila, increases expression at...