A5090792712- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Immune responses and vaccinations
- vaccines and immunoinformatics approaches
- Microbial infections and disease research
- Milk Quality and Mastitis in Dairy Cows
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Diagnosis and treatment of tuberculosis
- Adrenal Hormones and Disorders
- Ruminant Nutrition and Digestive Physiology
- Animal health and immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Probiotics and Fermented Foods
- Immunotherapy and Immune Responses
- Hormonal Regulation and Hypertension
- Animal Disease Management and Epidemiology
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- Fungal and yeast genetics research
- Pneumonia and Respiratory Infections
- Biofuel production and bioconversion
- Anaerobic Digestion and Biogas Production
AgResearch
2013-2023
New Zealand Agricultural Greenhouse Gas Research Centre
2013
Cardiff University
1998
University of Wales
1992-1994
Massey University
1986-1989
Cattle may provide a suitable model for testing ways of improving tuberculosis vaccine efficacy in human infants. A vaccination and challenge study was undertaken calves to determine the optimal time vaccinate neonatal animals with Mycobacterium bovis bacillus Calmette-Guérin (BCG) protection against whether revaccination BCG beneficial. Calves (10 per group) were vaccinated within 8 h birth or at 6 weeks age, when immune responses antigens environmental mycobacteria detectable, revaccinated...
Polyhydroxyalkanoates (PHAs) are biological polyesters that can be naturally produced by a range of bacteria as water-insoluble inclusions composed PHA core coated with synthesis, structural, and regulatory proteins. These self-assembling shell–core particles have been recently conceived biomaterials bioengineered biologically active beads for medical applications. Protein engineering PHA-associated proteins enabled the production PHA–protein assemblies exhibiting protein-based functions...
The variable efficacy of bacillus Calmette-Guérin (Mycobacterium bovis BCG) in protecting humans and cattle against tuberculosis has prompted a search for more effective vaccination regimen. A prime-boost strategy was investigated naturally sensitized to environmental mycobacteria by using combination three DNA vaccines coding Hsp 65, 70, Apa priming, followed boost with BCG prior experimental challenge virulent M. bovis. Controls were vaccinated or alone not vaccinated. immune responses...
An adrenal-specific protein reacting with autoantibodies in the sera of patients adult onset Addison's disease has been purified from human adrenal glands. The protein, mol.wt. 55K, biochemical characteristics steroid 21-hydroxylase and reacts on Western blots rabbit antibodies to recombinant 21-hydroxylase. Absorption native 55K prevented subsequent reaction blot analysis. In addition, reacted expressed yeast. These data indicate that specific enzyme is a major autoantigen involved...
ABSTRACT New improved vaccines are needed for control of both bovine and human tuberculosis. Tuberculosis protein have advantages with regard to safety ease manufacture, but efficacy against tuberculosis has been difficult achieve. Protective cellular immune responses can be preferentially induced when antigens displayed on small particles. In this study, Escherichia coli Lactococcus lactis were engineered produce spherical polyhydroxybutyrate (PHB) inclusions which a fusion Mycobacterium ,...
Bioengineered bacterial polyester inclusions have the potential to be used as a vaccine delivery system. The biopolyester beads were engineered display fusion protein of synthase PhaC and two key antigens involved in immune response infectious agent that causes tuberculosis, Mycobacterium notably antigen 85A (Ag85A) 6-kDa early secreted antigenic target (ESAT-6) from tuberculosis. Polyester displaying respective at high density successfully produced (henceforth called Ag85A-ESAT-6 beads) by...
In both humans and animals, controversy exists concerning the duration of protection induced by BCG vaccine against tuberculosis (TB) whether revaccination enhances protection. A long-term study was undertaken to determine BCG-vaccinated calves would be protected challenge with Mycobacterium bovis 2½ years after vaccination effect 2 years. Seventy–nine were divided into five groups (n = 15–17 calves/group) four vaccinated subcutaneously 105 CFU Danish at 2–4 weeks age fifth group serving as...
The development of a subunit protein vaccine for bovine tuberculosis which could be used either in combination with Mycobacterium bovis BCG (to improve the efficacy that vaccine) or alone would offer significant advantages over currently available strategies. A study was conducted cattle to determine protective strategy based on concurrent immunization an M. culture filtrate (CFP) and compared vaccination alone. One group calves (10 animals per group) vaccinated subcutaneously CFP formulated...
ABSTRACT Priming neonatal calves at birth with a Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine and boosting DNA consisting of plasmids encoding mycobacterial antigens Hsp65, Hsp70, Apa or the reverse prime-boost sequence induced similar levels protection against experimental challenge . When M. was isolated from thoracic lymph node following challenge, two groups given regimen had significantly lower numbers isolates than those vaccinated BCG alone. These observations suggest...
Current efforts are aimed at optimizing the protective efficacy of Mycobacterium bovis BCG by use vaccine combinations. We have recently demonstrated that protection afforded alone is enhanced vaccinating cattle with a combination vaccines comprising and protein tuberculosis vaccine, namely, culture filtrate proteins (CFPs) from M. plus an adjuvant. In current study, three different adjuvant systems were compared. The CFP was formulated depot adjuvant, dimethyldioctadecyl ammonium bromide...
Abstract AIM: To develop an understanding of the immune responses ruminants to methanogens, and provide proof a concept that harnessing system is potentially viable approach mitigate greenhouse gas emissions from agriculture. METHODS: Four subcellular fractions, namely cytoplasmic, two cell-wall preparations, cell wall-derived proteins were prepared Methanobrevibacter ruminantium M1. Twenty sheep (10 months age) vaccinated with these fractions or whole cells (n=4 per group). Sheep...