A5101959830- Bone Metabolism and Diseases
- Mosquito-borne diseases and control
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Viral Infections and Vectors
- Curcumin's Biomedical Applications
- Mesenchymal stem cell research
- HIV Research and Treatment
- RNA modifications and cancer
- Malaria Research and Control
- Bone health and treatments
- Bone and Dental Protein Studies
- Wound Healing and Treatments
- FOXO transcription factor regulation
- Viral Infections and Outbreaks Research
- Cancer-related gene regulation
- Skin Protection and Aging
- Congenital heart defects research
- Bone Tissue Engineering Materials
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Kruppel-like factors research
- Hippo pathway signaling and YAP/TAZ
- Long-Term Effects of COVID-19
- Osteomyelitis and Bone Disorders Research
Fujian Medical University
2023-2024
Novartis (Singapore)
2007-2021
Peking Union Medical College Hospital
2018-2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2021
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2020
Institute of Molecular and Cell Biology
2004-2014
Abstract The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms, but the underlying mechanism remains unclear. We conducted a randomized, single-blind, placebo-controlled (29 patients/group) phase II trial to validate previous findings and explore potential mechanisms. Patients treated umbilical cord-derived MSCs exhibited shorter hospital stay ( P = 0.0198) less time required for symptoms remission 0.0194) than...
Dengue fever is an emerging arboviral disease for which no vaccine or antiviral treatment exists and that causes thousands of fatalities each year. To develop in vivo test system antidengue drugs, AG129 mice, are deficient the interferon- alpha / beta - gamma receptors, were injected with unadapted dengue virus, resulting a dose-dependent transient viremia lasting several days peaking on day 3 after infection. Additionally, nonstructural protein 1, increased levels proinflammatory cytokines,...
The four serotypes of dengue virus (DENV-1 to -4) represent the most prevalent mosquito-borne viral pathogens in humans. No clinically approved vaccine or antiviral is currently available for DENV. Here we report a spiropyrazolopyridone compound that potently inhibits DENV both vitro and vivo. inhibitor was identified through screening 1.8-million-compound library by using DENV-2 replicon assay. selectively -3 (50% effective concentration [EC50], 10 80 nM) but not DENV-1 -4 (EC50,>20 M)....
Dengue virus (DENV) is a mosquito-borne flavivirus that poses threat to public health, yet no antiviral drug available. We performed high-throughput phenotypic screen using the Novartis compound library and identified candidate chemical inhibitors of DENV. This series was optimized improve properties such as anti-DENV potency solubility. The lead compound, NITD-688, showed strong against all four serotypes DENV demonstrated excellent oral efficacy in infected AG129 mice. There 1.44-log...
As the population ages, medical and socioeconomic impact of age-related bone disorders will further increase. An imbalance between osteogenesis adipogenesis mesenchymal stem cells (MSCs) can lead to various metabolic diseases such as osteoporosis. Thus, understanding molecular mechanisms underlying MSC osteogenic adipogenic differentiation is important for discovery novel therapeutic paradigms these diseases. miR-10b has been widely reported in tumorigenesis, cancer invasion metastasis....
With the expansion of elderly population, age-related osteoporosis and resulting bone loss have become a significant health socioeconomic issue. In Triple Energizer (TE)/San Jiao (SJ)/mesenchymal tissue system, mesenchymal stem cell (MSC) senescence, impaired osteogenesis are thought to contribute diseases such as osteoporosis. Therefore, comprehending molecular mechanisms underlying MSC senescence is essential improve treatment metabolic diseases. increasing role miRNAs in aging osteogenic...
Osteoporosis is characterized by deterioration of bone microarchitecture and low mass. One the primary causes osteoporosis decrease in osteogenic differentiation mesenchymal stem cells (MSCs). Tissue engineering therapy with genetically modified MSCs has attracted much attention study regeneration. In this study, we found that expression level miR-450b was upregulated during human adipose-derived (hADSCs). To explore effect on osteogenesis hADSCs, performed a series gain- loss-of-function...
Curcumin ameliorates bone loss by inhibiting osteoclastogenesis. inhibits RANKL-promoted autophagy in osteoclast precursors (OCPs), which mediates its anti-osteoclastogenic effect. But the role of RANKL signaling curcumin-regulated OCP is unknown. This study aimed to explore relationship between curcumin, signaling, and during We investigated curcumin RANKL-related molecular OCPs, identified significance RANK-TRAF6 curcumin-treated osteoclastogenesis using flow sorting lentiviral...
Notch signaling pathway defines an evolutionarily conserved mechanism in cell-fate determination a broad spectrum of developmental processes through local cell interactions. mind bomb (mib) encodes E3 ubiquitin ligase that is involved activation Delta ubiquitylation and internalization. To further dissect the function Mib, two yeast two-hybrid screens for zebrafish Mib/Mib2-binding proteins with different strategies have been performed. 81 putative interesting were discovered classified into...
Curcumin, a natural phenolic biphenyl compound derived from the plant Curcuma longa, modulates multiple steps of carcinogenesis partly by affecting expression miRNAs.Interestingly, cancer development shares many same signalling pathways with bone formation.Reduced mass creates favourable conditions for tumor metastasis.However, effects and mechanism curcumin on formation osteogenesis are relatively unknown controversial.We demonstrated that inhibited human adiposederived mesenchymal stem...
Nowadays, immune diseases are a large burden in healthcare. Mesenchymal stem cells (MSCs) have prominent ability immunomodulation and been applicated on treating many immune-related diseases. However, the clinical outcomes can be disparate sometimes completely counterproductive beyond explanation of cell heterogeneity. The theory plasticity MSCs has then emerged to explain that induced into proinflammatory MSC1 or anti-inflammatory MSC2 responding different environment. It would safer more...
Abstract The generation of definitive endoderm (DE) cells in sufficient numbers is a prerequisite for cell-replacement therapy liver and pancreatic diseases. Previously, we reported that human adipose-derived mesenchymal stem (hAMSCs) can be induced to DE lineages subsequent functional cells. Clarifying the regulatory mechanisms underlying fate conversion from hAMSCs helpful developing new strategies improve differentiation efficiency organs. Long noncoding RNAs (lncRNAs) have been shown...
Obesity has received increasing attention because of its widespread worldwide occurrence and many threats to health. Human adipose-derived mesenchymal stem cells (hADSCs) are a critical source adipocytes. Long noncoding RNAs (lncRNAs) play pivotal roles in cell fate determination differentiation. The objective the present study was identify investigate function regulatory mechanism lncRNAs on adipogenic differentiation hADSCs. We used lncRNA arrays prominent differentially expressed before...
Abstract Drosophila fringe and its homologues in vertebrates code for glycosyltransferases that modify Notch, altering the sensitivity of this receptor protein to ligands Delta Serrate and, thereby, playing an essential part demarcation tissue boundaries. We describe isolation characterization three zebrafish ( Danio rerio ) homologues: lunatic lfng ), radical rfng manic mfng ). In addition sites previously described (Prince et al. [ 2001 ] Mech. Dev. 105:175–180; Leve Genes Evol....
Long noncoding RNAs are crucial factors for modulating adipogenic differentiation, but only a few have been identified in humans. In the current study, we previously unknown human long RNA, LYPLAL1-antisense RNA1 (LYPLAL1-AS1), which was dramatically upregulated during differentiation of adipose-derived mesenchymal stem cells (hAMSCs). Based on 5' and 3' rapid amplification cDNA ends assays, full-length LYPLAL1-AS1 523 nt. Knockdown decreased hAMSCs, whereas overexpression enhanced this...
Photoaging is a degenerative biological process. As kind of pluripotent stem cells, adipose-derived cells (ADSCs) are widely used in the treatment photoaging. Therefore, we aimed to find an effective way improve antiaging ability ADSCs. In this study, isolated ADSCs and assessed multilineage differentiation markers. Cultured were preconditioned with astragaloside IV (ASI) at 10-7, 10-6, 10-5 M. Cell proliferation was by CCK-8 assay cytokine secretion enzyme-linked immunosorbent (ELISA). A...
Monophosphate prodrug analogs of 2′-deoxy-2′-fluoro-2′- C -methylguanosine have been reported as potent inhibitors hepatitis virus (HCV) RNA-dependent RNA polymerase. These prodrugs also display anti-dengue activities in cellular assays although their moieties were designed to produce high levels triphosphate the liver. Since peripheral blood mononuclear cells (PBMCs) are among major targets dengue virus, different effectively deliver monophosphate and corresponding triphosphates into PBMCs...
Background: Curcumin, a natural phenolic biphenyl compound derived from the plant Curcuma longa, modulates multiple steps of carcinogenesis, including inhibiting cell proliferation, induction apoptosis, and amelioration resistance to chemotherapeutic agents, partly by affecting expression miRNAs. Interestingly, cancer development shares many same signalling pathways with bone formation. Reduced mass creates favourable conditions for tumor metastasis. However, effects mechanism curcumin on...
Monophosphate prodrug analogs of 2’-deoxy-2’-fluoro-2’-C-methylguanosine have been reported as potent inhibitors hepatitis C virus (HCV) RNA-dependent RNA polymerase. These prodrugs also display anti-dengue activities in cellular assays although their moieties were designed to produce high levels triphosphate the liver. Since peripheral blood mononuclear cells (PBMCs) are one major targets dengue virus, different effectively deliver monophosphate and corresponding triphosphates into PBMCs...