A5033199827- Fungal and yeast genetics research
- Antifungal resistance and susceptibility
- Probiotics and Fermented Foods
- Wheat and Barley Genetics and Pathology
- Enterobacteriaceae and Cronobacter Research
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Fungal Infections and Studies
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Genomics, phytochemicals, and oxidative stress
- Plant nutrient uptake and metabolism
- DNA Repair Mechanisms
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
Western Sydney University
2020-2023
University of Exeter
2015-2020
Medical Research Council
2018
University of Aberdeen
2012-2018
The advent of the genomic era has made elucidating gene function on a large scale pressing challenge. ORFeome collections, whereby almost all ORFs given species are cloned and can be subsequently leveraged in multiple functional approaches, represent valuable resources toward this endeavor. Here we provide novel, genome-scale tools for study Candida albicans, commensal yeast that is also responsible frequent superficial disseminated infections humans. We have generated an collection composed...
The lack of techniques for rapid assembly gene deletion vectors, paucity selectable marker genes available genetic manipulation and low frequency homologous recombination are major constraints in construction mutants Zymoseptoria tritici. To address these issues, we have constructed ternary vectors Agrobacterium tumefaciens mediated transformation Z. tritici, which enable the single step multiple fragments via yeast recombinational cloning. sulfonylurea resistance gene, is a mutated allele...
Targeted gene deletion has been instrumental in elucidating many aspects of Zymoseptoria tritici pathogenicity. Gene over-expression is a complementary approach that amenable to rapid strain construction and high-throughput screening, which not exploited analyze Z. tritici, largely due lack available techniques. Here we exploit the Gateway® cloning technology for vectors improved homologous integration efficiency Δku70 build pilot library encompassing 32 genes encoding putative DNA binding...
Candida species are important pathogens of humans and the fourth most commonly isolated pathogen from nosocomial blood stream infections. Although albicans is principle causative agent invasive candidosis, incidence glabrata infections has rapidly grown. The reason for this increase not fully understood, but it clear that a higher innate tolerance to administered azole antifungals, in addition being highly tolerant stresses especially oxidative stress. Taking approach using model organism,...
Libraries of protein-encoding sequences can be generated by identification open reading frames (ORFs) from a genome choice that are then assembled into collections plasmids termed ORFeome libraries. These represent powerful resources to facilitate functional genomic characterization genes and their encoded products. Here, we report the generation an for Zymoseptoria tritici, which causes most serious disease wheat in temperate regions world. We screened strain IP0323 high confidence gene...
Abstract Hexokinases (HXK) were the first sugar signalling proteins identified in plants and are well known for their feedback regulation of photosynthetic gene expression. In some C 3 plants, HXKs have been found to regulate stomatal function. However, role HXK 4 photosynthesis, which is inherently more water use efficient than metabolism, remains poorly understood. Here, we report on tissue-specific modification a plant. SvHXK6 was expressed model grass Setaria viridis under control ZmPEPC...
ABSTRACT The advent of the genomic era has made elucidating gene function at large scale a pressing challenge. ORFeome collections, whereby almost all ORFs given species are cloned and can be subsequently leveraged in multiple functional approaches, represent valuable resources towards this endeavor. Here we provide novel, genome-scale tools for study Candida albicans , commensal yeast that is also responsible frequent superficial disseminated infections humans. We have generated an...
Abstract Libraries of protein-encoding sequences can be generated by identification open reading frames (ORFs) from a genome choice that are then assembled into collections plasmids termed ORFeome libraries. These represent powerful resources to facilitate functional genomic characterization genes and their encoded products. Here, we report the generation an for Zymoseptoria tritici , which causes most serious disease wheat in temperate regions world. We screened strain IP0323 high...