A5010821195- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
- Analytical Chemistry and Sensors
- Photoreceptor and optogenetics research
- Advanced X-ray Imaging Techniques
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Electrochemical Analysis and Applications
- Supramolecular Self-Assembly in Materials
- Laser-Plasma Interactions and Diagnostics
- Force Microscopy Techniques and Applications
- Crystallography and Radiation Phenomena
- Advanced Chemical Sensor Technologies
- Optical Coherence Tomography Applications
- Quantum optics and atomic interactions
- Diamond and Carbon-based Materials Research
- Photoacoustic and Ultrasonic Imaging
- Laser-Ablation Synthesis of Nanoparticles
- Molecular Junctions and Nanostructures
- Neural dynamics and brain function
- Laser Material Processing Techniques
- Advanced Optical Sensing Technologies
- Advanced Electron Microscopy Techniques and Applications
- Advanced Memory and Neural Computing
Bar-Ilan University
2014-2024
University of California, Berkeley
2024
Technion – Israel Institute of Technology
2016
We report clear experimental evidence for second harmonic generation at hard x-ray wavelengths. Using a 1.7 Å pumping beam generated by free electron laser, we observe in diamond. The is of order 10 times the background radiation, scales quadratically with pump pulse energy, and over narrow phase-matching condition. Of importance future experiments, our results indicate that it possible to nonlinear processes crystals intensities exceeding 1016 W/cm2.
Insertion of hydrophobic nanoparticles into phospholipid bilayers is limited to small particles that can incorporate a membrane core between two lipid leaflets. Incorporation above this size limit requires the development challenging surface engineering methodologies. In principle, increasing long-chain component in mixture should facilitate incorporation larger nanoparticles. Here, we explore effect incorporating very long phospholipids (C24:1) unilamellar vesicles on insertion efficiency...
We report the observation of parametrically down-converted x-ray signal photons at photon energies that correspond to idler optical wavelengths. The count-rate dependence on angles input beam and detector slit sizes agrees with theory within experimental uncertainties. nonlinear susceptibility, which we calculated from measured efficiencies, is comparable susceptibility evaluated measurements wave mixing. results present Letter advance development a spectroscopy method for probing...
Targeting individual cells within a heterogeneous tissue is key challenge in cancer therapy, encouraging new approaches for treatment that complement the shortcomings of conventional therapies. The highly localized interactions triggered by focused laser beams promise great potential targeting single or small cell clusters; however, most laser-tissue often involve macroscopic processes may harm healthy nearby and reduce specificity. Specific living using femtosecond pulses nanoparticles has...
We study the process of x-ray second-harmonic generation (SHG) from focused ultrashort pulses. numerically simulate and derive approximated analytical expressions for efficiency tolerances SHG process, considering effects temporal spatial walk-off, coupling to linear Bragg scattering. show that recently observed in diamond, simultaneous diffraction generated second harmonic is negligible. However, spatiotemporal walk-off are crucial components regarding wave propagation aspects this due...
Supported lipid bilayers are a well-developed model system for the study of membranes and their associated proteins, such as membrane channels, enzymes, receptors. These versatile can be made from various components, ranging simple synthetic phospholipids to complex mixtures constituents, mimicking cell with its relevant physiochemical molecular phenomena. In addition, high stability supported allows via wide array experimental probes. this work, we describe platform that is accessible both...
We describe a method for measuring the field profile of x-ray ultrashort pulses including phase information. The scheme is based on spectrally interfering two replicas same pulse, which are shifted via three-wave mixing with IR or visible beams. Using single-shot spectrometer can be used inspection individual random amplitudes and phases. Examples characterization stochastic bandwidth 1 eV given, criteria successful measurement.
Abstract Super-resolution optical imaging has become a prominent tool in life and material sciences, allowing one to decipher structures at increasingly greater spatial detail. Among the utilized techniques this field, super-resolution fluctuation (SOFI) proved be valuable approach. A major advantage of SOFI is its less restrictive requirements for generating super-resolved images neighboring nanostructures or molecules, as it only assumes that detected fluctuating light from emitters...
Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine developed utilized to optically record membrane potentials on single-nanodisc level. Ensemble single-nanosensor recordings were demonstrated HEK293 cells primary cortical neuron cells. Conjugation of anti-GABAA antibodies allowed site-specific potential...
Abstract Studying the electrical activity in single cells and local circuits of excitable cells, like neurons, requires an easy to use high throughput methodology that enables measurement membrane potential. properties particular sub-compartments or a specific type neurons produces additional complexity. An optical voltage-imaging technique allows spatial temporal resolution could be ideal solution. However, most valid voltage imaging techniques are nonspecific; The ones more site-directed...
Abstract Insertion of hydrophobic nanoparticles into phospholipid bilayers is limited to small particles that can incorporate the membrane core in between two lipid leaflets. Incorporation above this size limit requires development challenging surface engineering methodologies. In principle, increasing thickness should facilitate incorporation larger nanoparticles. Here, we explore effect incorporating very long phospholipids (C24:1) unilamellar vesicles on insertion efficiency are 5-13 nm...
The study of electrical activity in single cells and local circuits excitable cells, such as neurons, requires an easy-to-use, high-throughput methodology that allows for the measurement membrane potential. Investigating properties specific subcompartments or a type introduces additional complexity. An optical voltage-imaging technique high spatial temporal resolution could be ideal solution. However, most valid techniques are nonspecific. Those more site-directed require lot preliminary...
Ratiometric imaging is an invaluable tool for quantitative microscopy, allowing robust detection of FRET, anisotropy, and spectral shifts nano-scale optical probes in response to local physical chemical variations such as pH, ion composition, electric potential.In this paper, we propose demonstrate a scheme widefield ratiometric that allows continuous tuning the cutoff wavelength between its two channels.This based on angle-tuning image splitting dichroic beamsplitter, similar previous works...
Abstract Nanodisc technology was implemented as a platform for voltage nanosensors. A FRET-based sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine (DPA) developed utilized to optically record membrane potentials on single nanodisc level. Ensemble- single- nanosensor recordings were demonstrated HEK293 cells primary cortical neuron cells. Conjugation of anti-GABA-A antibodies allowed site specific potential measurements from post synaptic sites.
Abstract Supported lipid bilayers are a well-developed model system for the study of membranes and their associated proteins, such as membrane channels, enzymes, receptors. These versatile can be made from various components, ranging simple synthetic phospholipids to complex mixtures constituents, mimicking cell with its relevant physiochemical molecular phenomena. In addition, high stability supported allows via wide array experimental probes. this work, we describe platform that is...