A5036488239- Metabolomics and Mass Spectrometry Studies
- Physiological and biochemical adaptations
- Carcinogens and Genotoxicity Assessment
- Aquaculture Nutrition and Growth
- Food Industry and Aquatic Biology
- Skin Protection and Aging
- Cell Image Analysis Techniques
- Trace Elements in Health
- Molecular Biology Techniques and Applications
- 3D Printing in Biomedical Research
- Genetics, Bioinformatics, and Biomedical Research
- Environmental Toxicology and Ecotoxicology
- Heavy Metal Exposure and Toxicity
- Bioeconomy and Sustainability Development
- Advanced Proteomics Techniques and Applications
- Fish Ecology and Management Studies
- Helicobacter pylori-related gastroenterology studies
- Animal Genetics and Reproduction
- Gastrointestinal motility and disorders
- Mass Spectrometry Techniques and Applications
- S100 Proteins and Annexins
- Digestive system and related health
- Redox biology and oxidative stress
- Bacteriophages and microbial interactions
- Toxin Mechanisms and Immunotoxins
Institute of Biomedical Chemistry
2017-2021
Fish species exhibit great diversity rating of aging (from negligible to rapid), which gives a unique possibility for the discovery molecular mechanisms that determine differences in rate aging. A mass spectrometric metabolic profiling skeletal muscle fish with various rates was carried out by direct injection quadrupole time-of-flight spectrometer. The first group includes long-lived (pike (Esox Lucius) and sterlet (Acipenser ruthenus); second group—species gradual senescence such as...
There are a number of different animals that belong to long- and short-lived species show various rate ageing, providing an ideal model investigate mechanisms longevity. In this work, metabolome profiling blood plasma from fishes with ageing rates—negligible (Pike Esox Lucius Sterlet Acipenser ruthenus), gradual (Zander Sander lucioperca Perch Perca fluviatilis) rapid (Chum Salmon Oncorhynchus keta Pink gorbuscha)—was assessed by means direct infusion quadrupole time-of-flight mass...
Studies of genomes and transcriptomes are performed using sequencers that read the sequence nucleotide residues genomic DNA, RNA, or complementary DNA (cDNA). The analysis consists an experimental part (obtaining primary data) bioinformatic processing data. bioinformatics is with different sets input parameters. selection optimal values parameters, as a rule, requires significant computing power. article describes protocol for transcriptome data by virtual computers provided cloud platform...
Алгоритм АнАлизА рАспределения тяжелых метАллов в
Oxidative stress is a universal response of the skin cell damage various origins. Sodium dodecyl sulfate (SDS, sodium lauryl sulfate) an anionic surfactant commonly used as emulsifying detergent in household cleaners. reference compound for testing toxicity on cellular models. The effect sub toxic dose 25 μg/mL during 48 h protein profile human keratinocytes HaCaT was studied by tandem mass spectrometry with electrospray ionization. In total, 1064 proteins were found immortalized HaCaT,...
The effects of sodium dodecyl sulfate (25 mg/ml) and Triton X-100 (12.5 mg/ml 25 on the HaCaT immortalized keratinocytes exposed to these surfactants for 48 h were studied. Using shotgun proteomics, a comparative analysis proteomic profiles control experimental cells after exposure was carried out. 260 common proteins identified in cells; 33 found all three treatments, but not cells. These apparently reflect nonspecific (universal) response toxic damage by surfactants. are associated with...
OMERO service was used to annotate the cell line HaCaT microscope images by two independent expert groups. The were obtained in course of developing tissue-engineered epithelium which consisted several layers keratinocytes. Evaluation opinions performed calculation specificity, sensitivity and accuracy. best convergence (91%) achieved for confluence monolayers. Accuracy 70% observed determining extent differentiation after 10 days incubation. paper illustrates usefulness dynamic...