A5051315786- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Glycosylation and Glycoproteins Research
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Antibiotic Resistance in Bacteria
- Polymer Surface Interaction Studies
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
- Microfluidic and Capillary Electrophoresis Applications
- Antifungal resistance and susceptibility
- Doctoral Education Challenges and Solutions
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Photoreceptor and optogenetics research
- Peptidase Inhibition and Analysis
- Advanced Sensor and Energy Harvesting Materials
- Biochemical and Structural Characterization
- Advanced Chemical Sensor Technologies
- Animal Virus Infections Studies
- Marine Biology and Environmental Chemistry
- Ocular Surface and Contact Lens
- Career Development and Diversity
- Antibiotics Pharmacokinetics and Efficacy
Massachusetts Institute of Technology
2015-2025
Harvard–MIT Division of Health Sciences and Technology
2021
California Institute of Technology
2013
ABSTRACT Chronic, biofilm-like infections by the opportunistic pathogen Pseudomonas aeruginosa are a major cause of mortality in cystic fibrosis (CF) patients. While much is known about P. from laboratory studies, far less understood what it experiences vivo . Iron an important environmental parameter thought to play central role development and maintenance infections, for both anabolic signaling purposes. Previous studies have focused on ferric iron [Fe(III)] as target antimicrobial...
Binding of small molecules to mucus membranes in the body has an important role human health, as it can affect diffusivity and activity any molecule that acts a mucosal environment. The binding drugs toxins signaling from pathogens is particular clinical interest. Despite importance mucus–small binding, there lack data revealing precise chemical features lead binding. We developed novel equilibrium dialysis assay measure libraries mucin other components, substantially increasing throughput...
Mucus, a biopolymer hydrogel that covers all wet epithelia of the body, is potential site for infection by pathogenic bacteria. Mucus can bind small molecules and influence bacterial physiology, two factors may affect efficacy antibiotics. In spite this, impact mucus on antibiotic activity has not been thoroughly characterized. We examined polymyxin fluoroquinolone antibiotics against opportunistic pathogen Pseudomonas aeruginosa in native purified mucin environments. found reduces...
Conditional therapeutics that rely on disease microenvironment-specific triggers for activation are a promising strategy to improve therapeutic cargos. Among the investigated triggers, protease activity is used most often because of its dysregulation in several diseases. How optimally fine-tune different cargos remains challenge. Here, we designed nanobody-targeted conditional antimicrobial deliver model peptide and protein site bacterial infection. We explored parameters influence...
Abstract Breath biopsy is emerging as a rapid and non-invasive diagnostic tool that links exhaled chemical signatures with specific medical conditions. Despite its potential, clinical translation remains limited by the challenge of reliably detecting endogenous, disease-specific biomarkers in breath. Synthetic represent an paradigm for precision diagnostics such they amplify activity-based biochemical signals associated disease fingerprints. However, their adaptation to breath has been...