A5008992164- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lipid Membrane Structure and Behavior
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Inhalation and Respiratory Drug Delivery
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
Massachusetts Institute of Technology
2022-2025
Cornell University
2019
Lipid nanoparticles (LNPs) have great potential to enable inhaled delivery of mRNA treat pulmonary diseases. However, this has been limited by the challenge nebulizing LNPs. Nebulization LNPs can cause aggregate and release encapsulated mRNA, limiting their efficacy. To overcome challenge, are developed whereby PEG-lipid is fully replaced with a zwitterionic polymer (ZIP)-lipid conjugate greatly enhance nebulizer stability. formulated ZIP-lipids (ZIP-LNPs) were stable nebulization across...
Abstract There is an urgent need for affinity reagents that target phospho-modified sites on individual proteins; however, generating such remains a significant challenge. Here, we describe genetic selection strategy routine laboratory isolation of phospho-specific designed ankyrin repeat proteins (DARPins) by linking in vivo capture phosphorylated protein with antibiotic resistance Escherichia coli cells. The assay validated using existing panel DARPins selectively bind the...