Demid Khokhlov

ORCID: 0000-0003-0919-7762
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About
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Research Areas
  • Optical and Acousto-Optic Technologies
  • Photoacoustic and Ultrasonic Imaging
  • Optical measurement and interference techniques
  • Optical Coherence Tomography Applications
  • Advanced Vision and Imaging
  • Thermography and Photoacoustic Techniques
  • Physiological and biochemical adaptations
  • Zebrafish Biomedical Research Applications
  • Image Processing Techniques and Applications
  • Optical Imaging and Spectroscopy Techniques
  • Spectroscopy Techniques in Biomedical and Chemical Research
  • Optical Polarization and Ellipsometry
  • Surface Roughness and Optical Measurements
  • Thermoregulation and physiological responses
  • Calibration and Measurement Techniques
  • Congenital heart defects research
  • Advanced Measurement and Metrology Techniques
  • Optical Systems and Laser Technology
  • Systemic Sclerosis and Related Diseases
  • Advanced Optical Imaging Technologies
  • 3D Surveying and Cultural Heritage
  • Water Quality Monitoring Technologies
  • Diagnosis and Treatment of Venous Diseases
  • Non-Invasive Vital Sign Monitoring
  • High Entropy Alloys Studies

Federal State Budgetary Institution of Science "Scientific and Technological Center of Unique Instrumentation" of the Russian Academy of Sciences
2016-2024

Moscow Power Engineering Institute
2017-2024

Russian Academy of Sciences
2021

Bauman Moscow State Technical University
2017-2021

Institute of Immunology
2021

National Research University Higher School of Economics
2017-2019

We present, to the best of our knowledge, first experimental demonstration a new imaging system for in situ measurement two-dimensional (2D) distribution surface temperature microscopic specimens. The main component is an tandem acousto-optical tunable filter (TAOTF) synchronized with video camera. A set TAOTF spectroscopic images (up few hundreds) taken by fit measured spectral curves each pixel Planck radiation function and determine emissivity sample using gray body approximation. It...

10.1364/ol.41.000901 article EN publisher-specific-oa Optics Letters 2016-02-17

Spectral image filtration by means of acousto-optical tunable filters (AOTFs) has multiple applications. For its implementation, a few different optical schemes are in use. They differ quality, number coupling components, dimensions and alignment complexity. To choose the system AOTF-based spectral imager properly, many factors have to be considered. Though various acousto-optic (AO) been tested discussed, their comparative analysis not reported up now. In this study, we assembled four most...

10.3390/ma14112984 article EN Materials 2021-05-31

The capillary system immediately responds to many pathologies and environmental conditions. Accurate monitoring of its functioning often enables early detection various diseases related disorders in skin microcirculation. To expand the scope capillaroscopy application, it is reasonable visualize assess blood microcirculation exactly areas inflamed skin. Body vibrations, breathing, non-flat surface other factors hamper application conventional capillaroscopes outside nailfold area. In this...

10.1364/boe.420786 article EN cc-by Biomedical Optics Express 2021-06-24

We address the optical design procedure of prism-based stereoscopic imaging systems. Conventional approach includes two sequential stages: selection hardware and development proper digital image processing algorithms. At each these stages, specific techniques are applied, which almost unrelated to other. The main requirements system include only key parameters quality. Therefore, insufficient measurement accuracy may be revealed after prototype is assembled tested. In this case, even...

10.1364/oe.400428 article EN cc-by Optics Express 2020-07-21

Abstract We report on the noninvasive method for in vivo study of fish's cardiovascular system, that is, heart and structure vessels carry blood throughout body. The proposed approach is based combined photoplethysmographic videocapillaroscopic microscopic imaging enables noncontact two‐dimensional mapping volume changes. demonstrate obtained data allows precise measurements heartbeat, flow velocity other important parameters (see Videos S1 S2 ). To validate developed image processing...

10.1002/jbio.202000061 article EN Journal of Biophotonics 2020-04-19

The lack of oxygen (O2) causes changes in the cell functioning. Modeling hypoxic conditions vitro is challenging given that different types exhibit sensitivities to tissue O2 levels. We present an effective vivo platform for assessing various and organ parameters Danio rerio larvae under acute conditions. Our system allows simultaneous positioning multiple individuals within a chamber where level water can be precisely promptly regulated, all while conducting microscopy. applied this...

10.1089/ars.2024.0563 article EN Antioxidants and Redox Signaling 2024-08-01

Optical techniques are utilized for the non-invasive analysis of zebrafish cardiovascular system at early developmental stages. Being based mainly on conventional optical microscopy components and image sensors, wavelength range collected analyzed light is not out scope 400-900 nm. In this paper, we compared approaches utilizing visible near infrared (VISNIR) 400-1000 shortwave (SWIR) 900-1700 The transmittance spectra tissues were measured in these ranges, then vessel maps, heart rates,...

10.1038/s41598-022-25386-w article EN cc-by Scientific Reports 2022-12-03

Nowadays, stereoscopic devices are widely used for precise three-dimensional (3-D) measurements of hard-to-reach elements in industrial and biomedical applications. The most common approach their implementation is the utilization prism-based optical systems, which allows simultaneous acquisition two images from different viewpoints on a single sensor. Normally, they equipped with wideband (WB) white-light source, but contrast visualization inspected object and, therefore, accurate...

10.1117/1.oe.58.3.033104 article EN Optical Engineering 2019-03-20

Spectrally tunable illumination is widely used for colorimetry, spectroscopy, spectral-domain optical coherence tomography and other applications. Most of the swept light sources suffer either from a limited number spectral channels or spatial noise speckles. In this paper, we discuss an approach based on acousto-optic filtration wide-band illumination. We show experimentally that acousto-optical filter (AOTF) provides unique collection features: arbitrary tuning, multi-bandpass mode...

10.1117/12.2502756 article EN 2018-11-05

Identification of specific vascular patterns in skin formations is important for non-invasive differential diagnosis benign and malignant tissues. Accurate blood vessel mapping quantitative analysis the vessels morphology may increase diagnostic efficiency significantly comparison to conventional dermatoscopy methods. In this paper, we propose videocapillaroscopy technique visualization microvascular architecture right nevi area. It includes acquisition lesion images by exoscope-based...

10.1117/12.2621479 article EN 2022-05-19

10.3103/s106287381811014x article EN Bulletin of the Russian Academy of Sciences Physics 2018-11-01

The zebrafish (Danio rerio) is an increasingly popular animal model biological system. In cardiovascular research, it has been used to specific cardiac phenomena as well identify novel therapies for human disease. While the system functioning examined at larval stages, mechanisms by which vessel activity initiated remain a subject of intense investigation. this we report on in vivo stain-free blood imaging technique pre-larval stages embryonic development. We have developed algorithm...

10.3390/diagnostics10110886 article EN cc-by Diagnostics 2020-10-30

Zebrafish is a well-established animal model for developmental and disease studies. Its optical transparency at early stages allows in vivo tissues visualization. Interaction of polarized light with these provides information on their structure properties. This approach effective muscle tissue analysis due to its birefringence. To enable real-time Mueller-matrix characterization unanesthetized fish, we assembled microscope single-shot imaging. First, performed continuous observation 48...

10.1002/jbio.202200088 article EN Journal of Biophotonics 2022-05-18

We report on the calculation method for development of fiber-coupled acousto-optical (AO) spectral filtration modules compatible with conventional inexpensive wide-band light sources (incandescent lamps). The formulas main parameters optical systems such are given. Two possible schemes (collimating and telecentric confocal) AO discussed compared. Their throughput aberration analyzed. proposed approach may be useful tunable in various applications (microscopy, endoscopy, interferometry, etc.).

10.1117/12.2286647 article EN 23rd International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics 2017-11-30

Toxic environmental pollutants pose a health risk for both humans and animals. Accumulation of industrial contaminants in freshwater fish may become significant threat to biodiversity. Comprehensive monitoring the impact stressors on functional systems is important use non-invasive tools that can detect presence these toxicants vivo desirable. The blood circulatory system, by virtue its sensitivity external stimuli, could be an informative indicator chemical exposure. In this study,...

10.1002/jbio.202400111 article EN Journal of Biophotonics 2024-07-19

Monitoring the processes inside crystalline materials under their operating conditions is of great interest in optoelectronics and scientific instrumentation. Early defect detection ensures proper functioning multiple crystal-based devices. In this study, a combination acoustic emission (AE) sensing cross-polarization imaging proposed for fast characterization crystal’s structure. For experiments, tellurium dioxide (TeO2) crystal was chosen due to its wide use acousto-optics. Studies were...

10.3390/ma17143590 article EN Materials 2024-07-20

Postoperative bleeding is the most significant complication of tonsillectomy. Regular monitoring post-surgical wound healing in pharynx required. For this purpose, we propose endoscope-based non-invasive perfusion mapping and quantification. The combination imaging photoplethysmography image processing provides automated area selection microcirculation characterization. In feasibility study, demonstrate first results proposed approach to clinical trial on eight patients after Combination...

10.1002/jbio.202400248 article EN Journal of Biophotonics 2024-08-29
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