A5015261469- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Prostate Cancer Treatment and Research
- Sperm and Testicular Function
- Growth Hormone and Insulin-like Growth Factors
- Pregnancy and preeclampsia studies
- Cell Adhesion Molecules Research
- Neuroendocrine regulation and behavior
- Blood Coagulation and Thrombosis Mechanisms
- Endometriosis Research and Treatment
- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Preterm Birth and Chorioamnionitis
- Birth, Development, and Health
- Immune Cell Function and Interaction
- Molecular Biology Techniques and Applications
- Prostate Cancer Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and disorders
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Regulation of Appetite and Obesity
Helsinki University Hospital
2015-2024
University of Helsinki
2015-2024
Institute for Molecular Medicine Finland
2012
Johns Hopkins University
2010-2012
Karolinska Institutet
2010
358 (Finland)
2010
Finland University
2007
University of Eastern Finland
2007
GlaxoSmithKline (United Kingdom)
2006
Stratford University
2005
We assessed the magnitude of genetic component in variation circulating levels insulin-like growth factors I and II (IGF-I IGF-II), their binding proteins IGFBP-1 IGFBP-3 by measuring serum concentrations 32 monozygotic 47 dizygotic adult twin pairs same sex. The intrapair correlation for IGF-I was r = 0.41 (P < 0.009) twins 0.12 0.22) twins. For IGF-II concentration correlations were 0.66 0.0001) 0.34 0.01) No significant found either group. 0.65 0.23 0.06) twins, respectively. Women had...
Glycodelin, also known as placental protein 14 (PP14) or progesterone-associated endometrial (PAEP), is a human glycoprotein with potent immunosuppressive and contraceptive activities. In this paper we report the first characterization of glycodelin-derived oligosaccharides. Using strategies based upon fast atom bombardment electrospray mass spectrometry have established that glycodelin glycosylated at Asn-28 Asn-63. The site carries high mannose, hybrid complex-type structures, whereas...
We hypothesized that hyperinsulinemia contributes to early pregnancy loss in the polycystic ovary syndrome by adversely affecting endometrial function and environment. Serum glycodelin, a putative biomarker of function, is decreased women with loss. Insulin-like growth factor-binding protein-1 may also play an important role facilitating adhesion processes at feto-maternal interface. studied 48 before after 4 weeks administration 500 mg metformin (n = 26) or placebo 22) 3 times daily. Oral...
Acute physical exercise increases growth hormone (GH) secretion, and GH regulates the expression of insulin-like factor I (IGF-I) IGF-binding protein (IGFBP) 3. IGFBP-1 is a local modulator IGF activity with rapid dynamic regulation that downregulated by insulin. The system mediates metabolic actions GH, possibly it glucose metabolism. We hypothesize strenuous causes changes in system. studied effects marathon run on circulating levels IGF-I, IGFBP-1, IGFBP-3, insulin 23 participants....
Glycodelin-A is a human amniotic fluid-derived glycoprotein with contraceptive and immunosuppressive activities. An immunoreactive form of glycodelin was detected in seminal plasma over decade ago, but definitive characterization this not pursued. We considered it unlikely that the fertile men would contain an appreciable amount glycodelin-A. To address issue we purified (glycodelin-S) performed comparative studies Glycodelin-S behaved differently when compared glycodelin-A during sodium...
Tight control of sperm capacitation is important for successful fertilization. Glycodelin-S one the most abundant glycoproteins in human seminal plasma. However, its function unclear. We investigated role glycodelin-S on spermatozoa. Binding kinetics experiments demonstrated presence two saturable and reversible binding sites Differently glycosylated other isoforms glycodelin, glycodelin-A -F, did not compete with these sites, suggesting that are different from those isoforms. Indirect...
During placentation, the cytotrophoblast differentiates into villous and extravillous cytotrophoblast. The latter invades decidualized endometrium. Glycodelin-A (GdA) is abundantly synthesized by decidua but not trophoblast. Previous data indicate that GdA suppresses invasion of trophoblast cell lines down-regulating proteinase expression activities. This study addresses signaling pathway involved in above phenomenon. was found to suppress phosphorylation ERKs their downstream effector...
Does glycodelin-A (GdA) induce conversion of human peripheral blood CD16-CD56bright natural killer (NK) cells to decidual NK (dNK) facilitate placentation?GdA binds via its sialylated glycans and converts them a dNK-like cells, which in turn regulate endothelial cell angiogenesis trophoblast invasion vascular growth factor (VEGF) insulin-like factor-binding protein 1 (IGFBP-1) secretion, respectively.dNK are the most abundant leucocyte population decidua. These express phenotype. Peripheral...
Fertilization depends on successful binding of the spermatozoa to zona pellucida oocyte. Glycodelin-A inhibits spermatozoa-zona binding. Previous data showed that glycodelin-A receptor(s) and protein human are closely related. Using a chemical cross-linking approach, glycodelin-A-sperm receptor complex was isolated. The identified be fucosyltransferase-5 (FUT5) by mass spectrometry confirmed with use anti-FUT5 antibodies. Sperm FUT5 an externally oriented integral membrane in acrosomal...
Glycodelin is a human glycoprotein with four reported glycoforms, namely glycodelin-A (GdA), glycodelin-F (GdF), glycodelin-C (GdC), and glycodelin-S (GdS). These glycoforms have the same protein core appear to differ in their N-glycosylation. The glycosylation of GdA completely different from that GdS. inhibits proliferation induces cell death T cells. However, immunomodulating activities GdF GdC are not known. This study aimed use ultra-high sensitivity mass spectrometry compare glycomes...
Gestational diabetes mellitus (GDM) is a common metabolic disorder of pregnancy. Patients with GDM are at risk for high fetal mortality and gestational complications associated reduced immune tolerance abnormal carbohydrate metabolism. Glycodelin-A (GdA) an abundant decidual glycoprotein glycosylation-dependent immunomodulatory activities. We hypothesized that aberrant metabolism in was changes glycosylation GdA, leading to defective activities.GdA the amniotic fluid from women normal (NGdA)...
DNA damage response (DDR) pathways have been extensively studied in cancer cell lines and mouse models, but little is known about how recognized by different types nonmalignant, slowly replicating human tissues. Here, we assess, using ex vivo cultures of prostate tissue, DDR caused cytotoxic drugs (camptothecin, doxorubicin, etoposide, cisplatin) ionizing radiation (IR) the context normal tissue architecture. Using specific markers for basal luminal epithelial cells, determine quantify...