A5022398213- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- Insect behavior and control techniques
- Plant Reproductive Biology
- Insect-Plant Interactions and Control
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect Utilization and Effects
- Photosynthetic Processes and Mechanisms
- Animal Genetics and Reproduction
- Silk-based biomaterials and applications
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Olfactory and Sensory Function Studies
- Anodic Oxide Films and Nanostructures
- Botanical Research and Chemistry
- Genetically Modified Organisms Research
- Molecular Biology Techniques and Applications
California Institute of Technology
2018-2024
University of Göttingen
2013-2020
Significance There is great interest in spreading beneficial traits throughout wild populations self-sustaining ways. Here, we describe a synthetic selfish genetic element, CleaveR [Cleave and Rescue ( ClvR )], that simple to build can spread linked gene high frequency populations. composed of two components. The first, germline-expressed Cas9 guide RNAs (gRNAs), cleave disrupt versions an essential located elsewhere the genome. second, version resistant cleavage, provides function. spreads...
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits unique ability invade adapt ecological niches throughout tropical subtropical regions the world, though medfly infestations have been prevented controlled by sterile technique (SIT) as part integrated management programs (IPMs). genetic analysis manipulation has subject intensive study in an effort improve SIT...
Chemoreception is based on the senses of smell and taste that are crucial for animals to find new food sources, shelter, mates. The initial step in olfaction involves translocation odorants from periphery through aqueous lymph olfactory sensilla odorant receptors most likely by chemosensory proteins (CSPs) or binding (OBPs). To better understand roles CSPs OBPs a coleopteran pest species, red flour beetle Tribolium castaneum (Coleoptera, Tenebrionidae), we performed transcriptome analyses...
Significance Homing endonuclease gene (HEG)-based drive can bring about population suppression when genes required for viability or fertility are targeted. However, these strategies vulnerable to failure through mechanisms that create alleles resistant cleavage but retain wild-type function. We show resistance allele creation be prevented the use of guide RNAs designed cleave a at four target sites. homing rates were modest, and HEGs unstable during homing. In addition, promoter active in...
The red flour beetle Tribolium castaneum has emerged as an important model organism for the study of gene function in development and physiology, ecological evolutionary genomics, pest control a plethora other topics. RNA interference (RNAi), transgenesis genome editing are well established resources genome-wide RNAi screening have become available this model. All these techniques depend on high quality assembly precise models. However, first version was generated by Sanger sequencing, with...
The red flour beetle Tribolium castaneum is an emerging insect model organism representing the largest order, Coleoptera, which encompasses several serious agricultural and forest pests. Despite ecological economic importance of beetles, most olfaction studies have so far focused on dipteran, lepidopteran, or hymenopteran systems. Here, we present first detailed morphological description a coleopteran olfactory pathway in combination with genome-wide expression analysis relevant gene...
Significance Gene drive can spread beneficial traits through populations, but will never be a one-shot project in which one genetic element provides all desired modifications, for an indefinitely long time. Here, we show that gene drive-mediated population modification Drosophila overwritten with new content while eliminating old, using Cleave and Rescue ( ClvR ) selfish elements. The ability to carry out cycles of create then leave behind modest footprint entering exiting important points...
Canonical Wnt signaling has been implicated in an AP axis polarizing mechanism most animals, despite limited evidence from arthropods. In the long-germ insect, Drosophila , is not required for global patterning, but short-germ insects including Tribolium castaneum loss of affects development segments growth zone those defined blastoderm. To determine effects ectopic signaling, we analyzed expression and function axin which encodes a highly conserved negative regulator pathway. We found...
Gene drive elements promote the spread of linked traits, providing methods for changing composition or fate wild populations. Drive mechanisms that are self-limiting attractive because they allow control over duration and extent trait in time space, reversible through natural selection as wanes. Self-sustaining Cleave Rescue ( ClvR ) include a DNA sequence-modifying enzyme such Cas9/gRNAs disrupts endogenous versions an essential gene, tightly recoded version gene resistant to cleavage (the...
Wnt/β-catenin and hedgehog (Hh) signaling are essential for transmitting signals across cell membranes in animal embryos. Early patterning of the principal insect model, Drosophila melanogaster, occurs syncytial blastoderm, where diffusion transcription factors obviates need pathways. However, cellularized growth zone typical short germ embryos, pathways predicted to play a more fundamental role. Indeed, pathway is required posterior elongation most arthropods, although which target genes...
Summary Insect embryos complete the outer form of body via dorsal closure (DC) epidermal flanks, replacing transient extraembryonic (EE) tissue. Cell shape changes and morphogenetic behavior are well characterized for DC in Drosophila, but these data represent a single species with secondarily reduced EE component (the amnioserosa) that is not representative across insects. Here, we examine red flour beetle, Tribolium castaneum, providing first detailed, functional analysis an insect tissues...
One strategy for population suppression seeks to use gene drive spread genes that confer conditional lethality or sterility, providing a way of combining modification with suppression. Stimuli potential interest could be introduced by humans, such as an otherwise benign virus chemical, occur naturally on seasonal basis, change in temperature.
Abstract Background The Drosophila larval head is evolutionarily derived at the genetic and morphological level. In beetle Tribolium castaneum , development of more closely resembles ancestral arthropod condition. Unlike in a knirps homologue ( Tc-kni ) required for antennae mandibles. However, published data are restricted to cuticle phenotypes Tc-even-skipped Tc-wingless stainings knockdown embryos. Hence, it has remained unclear whether entire antennal mandibular segments depend on...
Abstract Gene drive elements promote the spread of linked traits, even when their presence confers a fitness cost to carriers, and can be used change composition or fate wild populations. Cleave Rescue ( ClvR ) sit at fixed chromosomal position include DNA sequence-modifying enzyme such as Cas9/gRNAs (the Cleaver/Toxin) that disrupts endogenous versions an essential gene, recoded version gene resistant cleavage Rescue/Antidote). spreads by creating conditions in which those lacking die...
Abstract Self-limiting gene drive allows control over the spread and fate of linked traits. Cleave Rescue ( ClvR ) elements create self-sustaining comprise a DNA sequence-modifying enzyme (Cas9-gRNAs, Cleaver that disrupts an essential gene, tightly linked, uncleavable version ). spreads by creating conditions in which those without it die because they lack function. We show when is implemented 2-locus format, with key – (and Cargo), Cas9 and/or gRNAs located at different genomic positions,...
Abstract A gene drive method of particular interest for population suppression utilizes homing endonuclease genes (HEGs), wherein a site-specific nuclease-encoding cassette is copied, in the germline, into target whose loss function results viability or fertility homozygous, but not heterozygous progeny. Earlier work Drosophila and mosquitoes utilized HEGs consisting Cas9 single gRNA that together specific cleavage. Homing was observed, resistant alleles, immune to cleavage, while retaining...
Abstract Background: The red flour beetle Tribolium castaneum has emerged as an important model organism for the study of gene function in development and physiology, ecological evolutionary genomics, pest control a plethora other topics. RNA interference (RNAi), transgenesis genome editing are well established resources genome-wide RNAi screening have become available this model. All these techniques depend on high quality assembly precise models. However, first version was generated by...
Abstract Gene drive-based strategies for modifying populations face the problem that genes encoding cargo and drive mechanism are subject to separation, mutational inactivation, loss of efficacy. Resilience, an ability respond these eventualities in ways restore population modification with functional is needed long-term success. Here we show resilience can be achieved through cycles “ Cleave Rescue” ( ClvR ) selfish genetic elements. comprises a DNA sequence-modifying enzyme such as...
Abstract Background: The red flour beetle Tribolium castaneum has emerged as an important model organism for the study of gene function in development and physiology, ecological evolutionary genomics, pest control a plethora other topics. RNA interference (RNAi), transgenesis genome editing are well established resources genome-wide RNAi screening have become available this model. All these techniques depend on high quality assembly precise models. However, first version was generated before...
ABSTRACT One strategy for population suppression seeks to use gene drive spread genes that confer conditional lethality or sterility, providing a way of combining modification with suppression. Stimuli potential interest could be introduced by humans, such as an otherwise benign virus chemical, occur naturally on seasonal basis, change in temperature. Cleave and Rescue ( ClvR ) selfish genetic elements Cas9 gRNAs disrupt endogenous versions essential gene, while also including version the...