A5010983473- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Treatments and Mutations
- Histone Deacetylase Inhibitors Research
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Biblical Studies and Interpretation
- German Literature and Culture Studies
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Insect behavior and control techniques
- Insect-Plant Interactions and Control
- interferon and immune responses
- Public Administration and Political Analysis
- CAR-T cell therapy research
- Genetic Associations and Epidemiology
- Insect Resistance and Genetics
- Cancer-related gene regulation
- GNSS positioning and interference
- European history and politics
- Retinal Diseases and Treatments
- SARS-CoV-2 detection and testing
St. Jude Children's Research Hospital
2019-2024
Booz Allen Hamilton (United States)
2022-2024
VA Boston Healthcare System
2024
Midwestern Baptist Theological Seminary
2023
GFZ Helmholtz Centre for Geosciences
2023
Institute of Bioinformatics and Applied Biotechnology
2020
Fraunhofer Institute for Microengineering and Microsystems
2020
University of California, Riverside
2016-2017
University of Tübingen
2011
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits unique ability invade adapt ecological niches throughout tropical subtropical regions the world, though medfly infestations have been prevented controlled by sterile technique (SIT) as part integrated management programs (IPMs). genetic analysis manipulation has subject intensive study in an effort improve SIT...
Abstract Eradication of acute myeloid leukemia (AML) is therapeutically challenging; many patients succumb to AML despite initially responding conventional treatments. Here, we showed that the imipridone ONC213 elicits potent antileukemia activity in a subset cell lines and primary patient samples, particularly stem cells, while producing negligible toxicity normal hematopoietic cells. suppressed mitochondrial respiration elevated α-ketoglutarate by suppressing dehydrogenase (αKGDH)...
Chimeric antigen receptor (CAR) T cell therapy for solid tumors has shown limited efficacy in early-phase clinical studies. The majority of CARs encode CD28 and/or 41BB costimulatory endodomains, and we explored whether MyD88 CD40 (MC) endodomains could improve their antitumor activity. We generated CD28-, 41BB-, MC-CAR cells demonstrated that have greater proliferative capacity activity repeat stimulation assays tumor models vivo. Transcriptomic analysis revealed expressed higher levels MYB...
Avidity is defined as the binding strength of immunoglobulin G (IgG) toward its target epitope. directly related to affinity, both processes are determined by best fit IgG epitopes. We confirm and extend data on incomplete avidity maturation severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP), spike protein-1 (S1), receptor-binding domain (RBD) in disease 2019 (COVID-19) patients. In SARS-CoV-2-infected individuals, an initial rise was ending abruptly, leading...
The serological responses towards severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein, receptor-binding domain (RBD), and spike protein S1 are characterized by incomplete avidity maturation. Analysis with varying concentrations of urea allows to determine distinct differences in maturation, though the total process remains at an unusually low level. Despite this approach define early late stages infection. It therefore can compensate for recently described irregular...
Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation, increased genomic instability replicative stress, defective homology-dependent DNA damage repair. Here show surprisingly distinct phenotypes result from G34V (also glioma) G34W (giant cell tumors...
DNA methylation at enhancers and CpG islands usually leads to gene repression, which is counteracted by demethylation through the TET protein family. However, how enzymes are recruited regulated these genomic loci not fully understood. Here, we identify TET2, glycosyltransferase OGT a previously undescribed proline serine rich protein, PROSER1 as interactors of UTX, component enhancer-associated MLL3/4 complexes. We find that mediates interaction between thus promoting TET2 O-GlcNAcylation...
Borna disease virus 1 (BoDV-1) causes rare but often fatal encephalitis in humans. Late diagnosis prohibits an experimental therapeutic approach. Here, we report a recent case of BoDV-1 infection diagnosed on day 12 after hospitalization by detection RNA the cerebrospinal fluid. In retrospective analysis, detect hospital admission when cell count fluid is still normal. We develop new ELISA using recombinant nucleoprotein, phosphoprotein, and accessory protein X to seroconversion 12. Antibody...
The COVID-19 pandemic has caused significant morbidity and mortality. Currently, there is a critical shortage of proven treatment options an urgent need to understand the pathogenesis multi-organ failure lung damage. Cytokine storm associated with severe inflammation organ damage during COVID-19. However, detailed molecular pathway defining this cytokine lacking, gaining mechanistic understanding how SARS-CoV-2 elicits hyperactive inflammatory response develop effective therapeutics. Of...
Fusion-positive rhabdomyosarcoma (FP-RMS) driven by the expression of PAX3-FOXO1 (P3F) fusion oncoprotein is an aggressive subtype pediatric rhabdomyosarcoma. FP-RMS histologically resembles developing muscle yet occurs throughout body in areas devoid skeletal highlighting that not derived from exclusively myogenic cell origin. Here we demonstrate P3F reprograms mouse and human endothelial progenitors to FP-RMS. We show aP2-Cre expressing cells functional stem capable regenerating injured...
Abstract PTEN promoter hypermethylation is nearly universal and copy number loss occurs in ~25% of fusion-negative rhabdomyosarcoma (FN-RMS). Here we show Pten deletion a mouse model FN-RMS results less differentiated tumors more closely resembling human embryonal RMS. activated the PI3K pathway but did not increase mTOR activity. In wild-type tumors, was expressed nucleus suggesting nuclear functions could account for these phenotypes. deleted had increased expression transcription factors...
The Helmholtz-Zentrum Potsdam GFZ German Research Centre for Geosciences is one of the Associate Analysis Centers International Doppler Orbitography and Radiopositioning Integrated by Satellite (DORIS) Service (IDS). In framework a recent reprocessing campaign Terrestrial Reference Frame (ITRF) suite DORIS-derived satellite products was extended improved with respect to reference frame determination. Among others Copernicus Sentinel-3A/3B 6A Michael Freilich (MF) satellites were used this...
Abstract To effectively reduce vision loss due to age-related macular generation (AMD) on a global scale, knowledge of its genetic architecture in diverse populations is necessary. A critical element, AMD risk profiles African and Hispanic/Latino ancestries, remains largely unknown lower lifetime prevalence. We combined clinical data the Million Veteran Program with five other cohorts conduct first multi-ancestry genome-wide association study discovered 63 loci (30 novel). observe marked...
Abstract IGH@ proto-oncogene translocation is a common oncogenic event in lymphoid lineage cancers such as B-ALL, lymphoma and multiple myeloma. Here, to investigate the interplay between IGH allelic exclusion, we perform long-read whole-genome transcriptome sequencing along with epigenetic 3D genome profiling of Nalm6, an - DUX4 positive B-ALL cell line. We detect significant imbalance on wild-type over haplotype expression data, showing occurs silenced allele. In vitro, this reduces stress...
In classical viral infections, the avidity of immunoglobulin G (IgG) is low during acute infection and high a few months later. As recently reported, SARS-CoV-2 infections are not following this scheme, but they rather characterized by incomplete maturation. This study was performed to clarify whether with seasonal coronaviruses also leads The IgG toward nucleoprotein (NP) 229E, NL63, OC43, HKU1 determined in sera from 88 healthy, SARS-CoV-2-negative subjects 70 COVID-19 outpatients, using...
Abstract Mosaic loss of chromosome Y (mLOY) is a common somatic mutation in leukocytes older males. mLOY was detected 126,108 participants the Million Veteran Program: 106,054 European (EUR), 13,927 admixed African (AFR), and 6,127 Hispanic. In multi-ancestry genome-wide association analysis, we identified 323 significant loci, 167 which were novel–more than doubling number known loci. Tract-based ancestry deconvolution resolved local inflation at AFR lead SNPs. Transcriptome-wide...
Abstract The humoral immune systems controls viral infections through recognition of critical target structures, selective proliferation stimulation IgM-presenting B cells, class switch to IgG-generating cells and subsequent affinity maturation IgG. Affinity is achieved proliferation, hypermutation clonal selection (1-4). establishment high IgG essential for sustained protective antiviral effects (5-10). While analyzing the functional (avidity) towards SARS CoV-2 nucleoprotein, surface...
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in humans could cause disease 2019 (COVID-19). Since its first discovery Dec 2019, SARS-CoV-2 has become a global pandemic and caused 3.3 million direct/indirect deaths (2021 May). Amongst the scientific community's response to COVID-19, data sharing emerged as an essential aspect of combat against SARS-CoV-2. Despite ever-growing studies about date, only few databases were curated enable access gene expression data....
Background: TERT promoter methylation, located several hundred base pairs upstream of the transcriptional start site, is cancer specific and correlates with increased mRNA expression poorer patient outcome. Promoter however, not mutually exclusive to activating genetic alterations, as predicted for functionally redundant mechanisms. To annotate altered patterns methylation their relationship gene expression, we applied a Pacific Biosciences-based, long-read, bisulfite-sequencing technology...
<b>Abstract ID 95704</b> <b>Poster Board 261</b> Acute myeloid leukemia (AML) overall survival rates remain unacceptably low. Hence, new drugs are needed to improve survival. The imipridone family of anticancer agents have shown efficacy against various cancers, including AML. Thus, we investigated the and mechanism action novel imipridone, ONC213, in preclinical AML models. cell lines primary patient samples showed varying degrees sensitivity ONC213. However, ONC213 uniformly suppressed...
Abstract DICER1 syndrome is a cancer predisposition where affected patients have increased risk of neoplasms including pleuropulmonary blastoma, Sertoli-Leydig cell tumor, and fusion-negative rhabdomyosarcoma (FN-RMS). This defined by germline heterozygous loss function mutations in the gene DICER1, but it remains unclear exactly how these predispose children to develop cancer. One would expect one allele provide sensitized background tumor acquire second hit mutation. To gain insight into...
<p>THP-1 metabolomics data</p>