A5030328836- Viral Infections and Vectors
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Rabies epidemiology and control
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Bat Biology and Ecology Studies
- Microbial infections and disease research
- Yersinia bacterium, plague, ectoparasites research
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Hepatitis B Virus Studies
- Zoonotic diseases and public health
- Animal Ecology and Behavior Studies
- RNA Interference and Gene Delivery
- Wildlife Ecology and Conservation
- COVID-19 Clinical Research Studies
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Outbreaks Research
Friedrich-Loeffler-Institut
2015-2024
World Health Organization
2020
University of California, Davis
2019
Kiel University
2018
Bernhard Nocht Institute for Tropical Medicine
2017-2018
Saarland University
2018
University Hospital Schleswig-Holstein
2018
University of Lübeck
2018
University Medical Center
2018
Justus-Liebig-Universität Gießen
2017
In December, 2019, a novel zoonotic severe acute respiratory syndrome-related coronavirus emerged in China. The syndrome 2 (SARS-CoV-2) became pandemic within weeks and the number of human infections cases is increasing. We aimed to investigate susceptibilty potential animal hosts risk anthropozoonotic spill-over infections.We intranasally inoculated nine fruit bats (Rousettus aegyptiacus), ferrets (Mustela putorius), pigs (Sus scrofa domesticus), 17 chickens (Gallus gallus domesticus) with...
In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 10-day-old lamb of the same breed flock presented progressive neurological signs including depressed sensorium, tremor, unusual behaviour. Neuropathological examination brain spinal cord detected non-suppurative polioencephalomyelitis dorsal root ganglionitis, characteristic neurotropic viral agent both sheep. Metagenomic analysis different tissue samples from animals identified novel Ovine Astrovirus (OvAstV). The presence...
Encephalitis can be caused by several infectious agents, including bacteria, fungi, parasites and viruses. In many cases, the causative agent cannot identified, because pathogens are unknown or detection methods not routinely available. our case, a 15-month-old cow developed central nervous disorders died within 6 days after onset of clinical signs. The histopathology revealed an acute encephalitis, predominantly in brain stem, ganglionitis trigeminal ganglion with massive neuronal necroses...
Rabies is a neglected zoonotic disease that causes an estimated 60,000 human deaths annually. The main burden lies on developing countries in Asia and Africa, where surveillance detection hampered by absence of adequate laboratory facilities and/or the difficulties submitting samples from remote areas to laboratories. Under these conditions, easy-to-use tests such as immunochromatographic assays, i.e. lateral flow devices (LFD), may increase improve control efforts. Several LFDs for rabies...
After many years of controversy, there is now recent and solid evidence that classical Borna disease virus 1 (BoDV-1) can infect humans. On the basis six brain autopsies, we provide first systematic overview on BoDV-1 tissue distribution lesion pattern in fatal BoDV-1-induced encephalitis. All brains revealed a non-purulent, lymphocytic sclerosing panencephalomyelitis with detection BoDV-1-typical eosinophilic, spherical intranuclear Joest–Degen inclusion bodies. While composition...
Abstract Combining optimized spike (S) protein-encoding mRNA vaccines to target multiple SARS-CoV-2 variants could improve control of the COVID-19 pandemic. We compare monovalent and bivalent encoding B.1.351 (Beta) and/or B.1.617.2 (Delta) S-protein in a transgenic mouse Wistar rat model. The blended low-dose vaccine contains half each respective vaccine, but induces comparable neutralizing antibody titres, enrichment lung-resident memory CD8 + T cells, antigen-specific CD4 responses,...
Approved vaccines are effective against severe COVID-19, but broader immunity is needed new variants and transmission. Therefore, we developed genome-modified live-attenuated (LAV) by recoding the SARS-CoV-2 genome, including 'one-to-stop' (OTS) codons, disabling Nsp1 translational repression removing ORF6, 7ab 8 to boost host immune responses, as well spike polybasic cleavage site optimize safety profile. The resulting OTS-modified LAVs, designated OTS-206 OTS-228, genetically stable can be...
Ancient genome sequences provide invaluable insights into the origin and evolution of viral pathogens, offering a broader temporal perspective that extends well beyond limited timespan clinical data, which typically covers at most few decades. Whereas ancient DNA is relatively frequently recovered, RNA genomes are scarce due to fragile nature molecules. In this study, we explored feasibility detecting in ethanol-preserved bat samples from museum collection. We not only detected fragments but...
Abstract Eradication of small ruminant morbillivirus (PPRV) is targeted for 2030. PPRV lineage IV found in much Asia and Africa. We used strain Kurdistan/2011 transmission trials to investigate the role pigs, wild boar, ruminants as reservoirs. Suids were a possible source infection.
Capripox virus (CaPV)-induced diseases (lumpy skin disease, sheeppox, goatpox) are described as the most serious pox of livestock animals, and therefore listed notifiable under guidelines World Organisation for Animal Health (OIE). Until now, only live-attenuated vaccines commercially available control CaPV. Due to numerous potential problems after vaccination (e.g., loss disease-free status respective country, possibility vaccine shedding transmission well risk recombination with field...
Abstract Background The detection of pathogens in clinical and environmental samples using high-throughput sequencing (HTS) is often hampered by large amounts background information, which especially true for viruses with small genomes. Enormous depth can be necessary to compile sufficient information identification a certain pathogen. Generic HTS combining in-solution capture enrichment markedly increase the sensitivity virus complex diagnostic samples. Methods A panel based on principle...
Borna disease virus 1 (BoDV-1) causes rare but often fatal encephalitis in humans. Late diagnosis prohibits an experimental therapeutic approach. Here, we report a recent case of BoDV-1 infection diagnosed on day 12 after hospitalization by detection RNA the cerebrospinal fluid. In retrospective analysis, detect hospital admission when cell count fluid is still normal. We develop new ELISA using recombinant nucleoprotein, phosphoprotein, and accessory protein X to seroconversion 12. Antibody...
Limbic encephalitis is commonly regarded as an autoimmune-mediated disease. However, after the recent detection of zoonotic variegated squirrel bornavirus 1 in a Prevost's (Callosciurus prevostii) zoo northern Germany, we retrospectively investigated fatal case autoantibody-seronegative animal caretaker who had worked at that zoo. The virus been discovered 2015 cause cluster cases among breeders squirrels (Sciurus variegatoides) eastern Germany. Molecular assays and immunohistochemistry...
Borna disease virus 1 (BoDV-1) is the causative agent of disease, an often fatal neurologic condition domestic mammals, including New World camelids, in endemic areas Central Europe. Recently, BoDV-1 gained further attention by confirmation zoonotic infections humans. Although and have been described already over past decades, comprehensive reports outbreaks animals employing state-of-the-art diagnostic methods are missing. Here, we report a series herd 27 alpacas (Vicugna pacos) federal...
Background: A novel zoonotic SARS-related coronavirus emerged in China at the end of 2019. The SARS-CoV-2 became pandemic within weeks and number human infections severe cases is increasing. role potential animal hosts still understudied.Methods: We intranasally inoculated fruit bats (Rousettus aegyptiacus; n=9), ferrets (n=9), pigs (n=9) chickens (n=17) with 105 TCID50 a isolate per animal. Animals were monitored clinically for virus shedding. Direct contact animals (n=3) included. humanely...
We screened squirrels in Germany and the Netherlands for novel zoonotic variegated squirrel bornavirus 1 (VSBV-1). The detection of VSBV-1 11 indicates a considerable risk transmission to humans handling those animals. Therefore, contact with should routinely be tested VSBV-1.
Peste-des-petits-ruminants virus (PPRV) causes a severe respiratory disease in small ruminants. The possible impact of different atypical host species the spread and planed worldwide eradication PPRV remains to be clarified. Recent transmission trials with virulent lineage IV (LIV)-strain Kurdistan/2011 revealed that pigs wild boar are sources PPRV-infection. We therefore investigated role cattle, llamas, alpacas, dromedary camels using strain for intranasal infection integrated literature...
Abstract Background Pneumonia and stomatitis represent severe often fatal diseases in different captive snakes. Apart from bacterial infections, paramyxo-, adeno-, reo- arenaviruses cause these diseases. In 2014, new viruses emerged as the of pneumonia pythons. a few publications, nidoviruses have been reported association with ball pythons tiger python. The were found using sequencing methods organ tissue dead animals. Methods Severe resulted high mortality rate breeding collection green...
African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread ASF, commercial kits have risen on market. In Germany, go through approval process thus, general validation can be assumed. However, they never been compared each other. this study, 12 were OIE-recommended method. Samples representing different matrices, genome loads, genotypes included in panel that was tested under...