A5020547706- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Retinoids in leukemia and cellular processes
- Receptor Mechanisms and Signaling
- Nerve injury and regeneration
- Proteoglycans and glycosaminoglycans research
- Connexins and lens biology
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Prostate Cancer Treatment and Research
- Cancer Immunotherapy and Biomarkers
- Neuroscience and Neuropharmacology Research
- ATP Synthase and ATPases Research
- Ion Transport and Channel Regulation
- Neuropeptides and Animal Physiology
- Skin Protection and Aging
- Cancer, Hypoxia, and Metabolism
- Pharmacological Receptor Mechanisms and Effects
- Pancreatic function and diabetes
- Muscle metabolism and nutrition
- Lipid Membrane Structure and Behavior
- Bladder and Urothelial Cancer Treatments
- Metabolism and Genetic Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
Huntsman Cancer Institute
2007-2024
University of Utah
2007-2024
All India Institute of Medical Sciences
2024
University of Colorado Anschutz Medical Campus
2015-2023
University of Colorado Denver
2015-2022
Cedars-Sinai Medical Center
2019-2022
Iora Ecological Solutions
2020
National Eye Institute
2008-2018
National Institutes of Health
2008-2018
Jamia Hamdard
2017
Thirteen mutant rhodopsins responsible for autosomal dominant retinitis pigmentosa (ADRP) have been produced by transfection of cloned cDNA into tissue culture cells. Three mutants [class I: Phe-45----Leu, Gln-344----termination (deletion C-terminal positions 344-348), and Pro-347----Leu] resemble wild-type rhodopsin in yield, regenerability with 11-cis-retinal, plasma membrane localization. Ten II: Thr-17----Met, Pro-23----His, Thr-58----Arg, Val-87----Asp, Gly-89----Asp, Gly-106----Trp,...
Abstract Emerging evidence indicates the presence of tumor-initiating cells (TIC) or cancer stem in osteosarcoma. However, no study has shown specific markers to identify osteosarcoma TICs with vivo tumor formation ability. Additionally, there been a lack investigations gauging contribution metastatic and drug-resistant properties. In this study, we have identified mouse human using mesenchymal cell CD117 Stro-1. These were preferentially expressed spheres doxorubicin-resistant cells. Both...
To examine an immortalized mouse retinal cell line (661W) for markers characteristic of photoreceptor cells and validate its origin.The 661W were cloned from tumors a transgenic that expresses the simian virus (SV) 40 T antigen under control human interphotoreceptor retinol-binding protein (IRBP) promoter. Morphologic, immunocytochemical, immunoblot analyses performed to characterize these cells. Total cellular was used analysis various photoreceptor-specific proteins.661W grew as monolayer...
Using subtraction cloning, we have isolated a human cDNA, AS321, which is expressed in retina and retinoblastoma cell lines but not any other tissue or line tested. AS321 mRNA detected all cells of neural retina, with high level expression photoreceptors. The polypeptide sequence deduced from the cDNA reveals consensus phosphorylation sites for protein kinase A proline-directed kinase. Its C-terminal region contains basic motif leucine zipper domain similar to DNA binding proteins jun fos...
Glaucomatous optic neuropathy causes blindness through the degeneration of retinal ganglion cells (RGCs) and their axons, which comprise nerve. Glaucoma traditionally is associated with elevated intraocular pressure, but often occurs or may progress pressure in normal range. Like other diseases CNS, a subset glaucoma has been proposed to involve an autoimmune component help explain loss RGCs absence pressure. One hypothesis involves heat shock proteins (HSPs), because increased serum levels...
In vivo–based functional genomic screen identifies DDR2 as an important determinant of efficacy anti–PD-1 immunotherapy.
Proximal tubule cells of the kidney contain, on their apical surface, an amiloride-sensitive Na/H antiporter that functions in Na reabsorption and proton secretion. We have investigated localization a cloned cell line porcine renal origin, LLC-PK1/Cl4, which is often considered to be useful model proximal tubule. Transport measurements were performed with differentiated monolayers grown Nuclepore filters, permitting independent access basolateral surfaces. In control experiments LLC-PK1/Cl4...
Mitochondrial fission is a cellular response to stress that has an important role in neuronal cell death neurodegenerative diseases. The purpose of this study was determine whether elevated hydrostatic pressure induces mitochondrial and dysfunction cultured retinal ganglion cells.RGC-5 cells were differentiated with succinyl concanavalin A (50 microg/mL) transferred pressurized incubator which 30 mm Hg applied for 1, 2, or 3 days. As control, from identical passage incubated simultaneously...
Previous work from the authors' laboratory has shown that Hsp27 is specifically upregulated after retinal ischemic preconditioning (IPC), and this upregulation acts as a key cytoprotective factor in preventing damage. The regulatory mechanisms involved of IPC are unknown. purpose study was to explore transcriptional events responsible for IPC.CoCl(2) used test expression hypoxic stimulus. promoter first intron regions human gene were cloned by PCR characterized deletion analysis using...
Mutations in the coding region of OPTN gene are associated with certain glaucomas. Although function optineurin protein is yet to be elucidated, most common mutation, E50K, a severe phenotype. This study explores some functional features and its mutants.Plasmids expressing normal or wild-type (WT) R545Q, H26D, H486R mutant were transfected into HeLa, Cos-1, IMR32, rat retinal ganglion cell (RGC) line RGC-5, their effects on survival monitored by morphologic observation cells studied....
Significance Telomerase reverse transcriptase (hTERT) is involved in immortalization and survival of cancer cells. Recurring mutations its promoter often lead to reexpression cancer. Therapies targeting hTERT activity have been challenging develop, none are routine use. Using targeted functional genomics knockout screening a human bladder model, we found that transcription factor TRIM28 activates expression preferentially from the mutant allele. We also revealed therapeutically targetable...
The mechanisms of photoreceptor cell death via apoptosis, in retinal dystrophies, are largely not understood. In the present report we show that visible light exposure mouse cultured 661W cells at 4.5 milliwatt/cm2 caused a significant increase oxidative damage cells, leading to apoptosis these cells. These constitutive expression nuclear factor-κB (NF-κB), and results lowering NF-κB levels both cytosolic fractions time-dependent manner. Immunoblot analysis IκBα p50, p65 (RelA) subunits...
The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is the target of insulin sensitizing thiazolidinediones (TZDs), a class drugs used in treatment type 2 diabetes mellitus. Glaucoma and other retinal disorders are some major complications diabetes. In present study, role that PPAR-gamma ligands play protecting ganglion cells (RGC-5) against glutamate insult was explored.Transformed rat RGC (RGC-5 cells) two agonists, 15-deoxy-D(12,14)-prostaglandin J2 (15d-PGJ2) troglitazone...
Apoptosis-related signaling pathways were investigated in a cultured rat retinal ganglion cell (RGC-5) line deprived of growth factors after serum withdrawal from the culture medium.RGC-5 cells subjected to deprivation for 2 6 days and compared with RGC-5 medium containing 10% fetal calf serum. Cell viability was determined by neutral red dye uptake assay. Apoptosis established DNA laddering. The expression various apoptosis-related genes immunoblot analysis, or reverse transcription...
Cell lines are frequently used to elucidate mechanisms of disease pathophysiology. Yet extrapolation results with cell neurodegenerative disorders is difficult because they mitotic and usually have other non-neuronal properties. The RGC-5 line has many features retinal ganglion cells (RGCs). Despite its expression Thy-1 NMDA receptors, as found in primary RGCs, this line's ability proliferate appearance differentiate it from central neurons, complicating use for the study neuronal survival,...