A5101836695- Connexins and lens biology
- Redox biology and oxidative stress
- Heat shock proteins research
- Advanced Glycation End Products research
- Aldose Reductase and Taurine
- Intraocular Surgery and Lenses
- Genomics, phytochemicals, and oxidative stress
- Biochemical effects in animals
- Endoplasmic Reticulum Stress and Disease
- Corneal Surgery and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Ocular Surface and Contact Lens
- Advances in Cucurbitaceae Research
- Ubiquitin and proteasome pathways
- Glutathione Transferases and Polymorphisms
- Genetics, Aging, and Longevity in Model Organisms
- Nitrogen and Sulfur Effects on Brassica
- Proteoglycans and glycosaminoglycans research
- MicroRNA in disease regulation
- Selenium in Biological Systems
- Glaucoma and retinal disorders
- Yersinia bacterium, plague, ectoparasites research
- Plant Pathogens and Fungal Diseases
- Curcumin's Biomedical Applications
- Retinal Development and Disorders
University of Nebraska Medical Center
2016-2025
Indiana University School of Medicine
2024-2025
Indiana University – Purdue University Indianapolis
2024-2025
Indiana University Health
2024-2025
Methodist Hospital
2025
University of Indianapolis
2025
Marymount University
2020
Visual Sciences (United States)
2014-2019
Southern University and Agricultural and Mechanical College
2017-2018
Govind Ballabh Pant University of Agriculture and Technology
1986-2017
Abstract Upon oxidative stress and aging, Nrf2 (NFE2-related factor2) triggers antioxidant defense genes to defends against homeostatic failure. Using human(h) or rat(r) lens epithelial cells (LECs) aging human lenses, we showed that a progressive increase in load during was linked decline Prdx6 expression. DNA binding experiments using gel-shift ChIP assays demonstrated reduction Nrf2/ARE (−357/−349) of promoter. The promoter (−918) with ARE marked young vs aged hLECs, which directly...
Antioxidant protein 2 (AOP2), a member of the newly defined family thiol-specific antioxidant proteins, has been shown to remove H2O2 and protect proteins DNA from oxidative stress. Here we report that LEDGF is one regulatory factors for AOP2 gene. We found bound heat shock element stress-related elements in promoter. It trans-activated expression AOP2-CAT COS-7 cells lens epithelial overexpressing LEDGF. Mutations promoter reduced LEDGF-dependent trans-activation. Lens showed higher level...
A diminished level of endogenous antioxidant in cells/tissues is associated with reduced resistance to oxidative stress. Peroxiredoxin 6 (PRDX6), a protective molecule, regulates gene expression/function by controlling reactive oxygen species (ROS) levels. Using PRDX6 protein linked TAT, the transduction domain from human immunodeficiency virus type 1 TAT protein, we demonstrated that was transduced into lens epithelial cells derived rat or mouse lenses. The biologically active, negatively...
Oxidative stress and endoplasmic reticulum (ER) are emerging as crucial events in the etiopathology of many neurodegenerative diseases. While neuroprotective contributions dietary compound curcumin has been recognized, molecular mechanisms underlying curcumin's neuroprotection under oxidative ER stresses remains elusive. Herein, we show that protects HT22 from evoked by hypoxia (1% O(2) or CoCl(2) treatment) enhancing peroxiredoxin 6 (Prdx6) expression. Cells exposed to displayed reduced...
Many disorders of aging, including blinding-diseases, are associated with deficiency brain and muscle arnt-like protein 1 (Bmal1) and, thereby, dysregulation antioxidant-defense pathway. However, knowledge is limited regarding the role Bmal1 regulation antioxidant-pathway in eye lens/lens epithelial cells (LECs) at molecular level. We found that, aging human (h)LECs, a progressive decline nuclear factor erythroid 2-related 2 (Nrf2)/ARE (antioxidant response element)-mediated antioxidant...
We report the isolation by RT-PCR of partial cDNAs encoding human peroxisome proliferator-activated receptor (PPAR) isoforms PPARβ and -γ in primary astrocytes (HPA) as well malignant astrocytoma cell line T98G. In contrast, we failed to detect PPARα mRNA either these two types. Because is ubiquitously expressed but has, yet, no known function, pursued our functional studies cells with regard PPARγ. To that end, showed PPARγ protein abundantly both types, having a molecular weight...
In addition to its role in virus entry, HIV-1 gp120 has also been implicated HIV-associated neurocognitive disorders. However, the mechanism(s) responsible for gp120-mediated neuroinflammation remain undefined. view of increased levels IL-6 HIV-positive individuals with neurological manifestations, we sought address whether is involved over-expression astrocytes. Transfection a human astrocyte cell line plasmid encoding resulted expression at mRNA and protein by 51.3±2.1 11.6±2.2 fold...
Peroxiredoxin 6 (Prdx6) is a pleiotropic oxidative stress-response protein that defends cells against reactive oxygen species (ROS)-induced damage. Curcumin, naturally occurring agent, has diversified beneficial roles including cytoprotection. Using human lens epithelial (hLECs) and Prdx6-deficient cells, we show the evidence curcumin protects by upregulating Prdx6 transcription via invoking specificity 1 (Sp1) activity proapoptotic stimuli. Curcumin enhanced Sp1 mRNA expression in...
Abstract A progressive decline in antioxidant potential and accumulation of reactive oxygen species (ROS) are major causes pathogenesis several diseases, including glaucoma. Trabecular meshwork (TM) dysfunction resulting higher intraocular pressure (IOP) is a hallmark glaucoma, but its unclear. Using human (h) TM cells derived from glaucomatous normal subjects different ages facing oxidative-stress, we showed that specific loss moonlighting protein Peroxiredoxin (Prdx) 6 aging or caused ROS...
Sulforaphane (SFN), an activator of transcription factor Nrf2 (NFE2-related factor), modulates antioxidant defense by Nrf2-mediated regulation genes like Peroxiredoxin 6 (Prdx6) and affects cellular homeostasis. We previously observed that dose levels SFN are crucial in determining life or death lens epithelial cells (LECs). Herein, we demonstrated higher doses (>6 μM) activated signaling overstimulation Nrf2/ARE (antioxidant response element)-mediated Kruppel-like (Klf9) repression Prdx6...
Triphala is an anti-oxidant-rich herbal formulation containing fruits of Emblica officinalis, Terminalia chebula and T. belerica in equal proportions. The preparation frequently used Ayurvedic medicine to treat diseases such as anaemia, jaundice, constipation, asthma, fever chronic ulcers. Anti-mutagenic effects the polyphenolic fractions isolated from have been reported, thus indicating that phenols present might be responsible for its therapeutic efficacy. A simple high-performance liquid...
Abstract Background The ability to respond changes in the extra-intracellular environment is prerequisite for cell survival. Cellular responses include elevating defense systems, such as antioxidant system. Hypoxia-evoked reactive oxygen species (ROS)-driven oxidative stress an underlying mechanism of retinal ganglion (RGC) death that leads blinding disorders. protein peroxiredoxin 6 (PRDX6) plays a pleiotropic role negatively regulating signaling response stressors, and thereby stabilizes...
The multifunctional cytoprotective protein peroxiredoxin 6 (Prdx6) maintains cellular homeostasis and membrane integrity by regulating expression of intracellular reactive oxygen species (ROS) phospholipid turnover. Using cells derived from targeted inactivation Prdx6 gene or its depletion RNA interference aging, we showed that deficiency in evoked unfolded response (UPR), evidenced increased activation proapoptotic factors, CHOP, ATF4, PERK, IRE-α eIF2-α caspases 3 12 processing. Those...
Oxidative stress or reduced expression of naturally occurring antioxidants during aging has been identified as a major culprit in neuronal cell/tissue degeneration. Peroxiredoxin (Prdx) 6, protective protein with GSH peroxidase and acidic calcium-independent phospholipase A2 activities, acts rheostat regulating cellular physiology by clearing reactive oxygen species (ROS) thereby optimizing gene regulation. We found that under stress, the cells displayed Prdx6 mRNA increased levels ROS,...