A5051819621- Plasma and Flow Control in Aerodynamics
- Gas Dynamics and Kinetic Theory
- Computational Fluid Dynamics and Aerodynamics
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- Plasma Diagnostics and Applications
- Combustion and Detonation Processes
- Laser-induced spectroscopy and plasma
- Laser-Plasma Interactions and Diagnostics
- Digital Holography and Microscopy
- Protist diversity and phylogeny
- Fluid Dynamics and Turbulent Flows
- DNA Repair Mechanisms
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Electromagnetic Launch and Propulsion Technology
- Advanced Optical Imaging Technologies
- Image Processing Techniques and Applications
- Laser Design and Applications
- Cell Image Analysis Techniques
- Ionosphere and magnetosphere dynamics
- Immune Response and Inflammation
- Porphyrin and Phthalocyanine Chemistry
- Microbial Community Ecology and Physiology
National Institute of Information and Communications Technology
2015-2024
Meijo University
2015-2024
Takeda (Japan)
2017-2024
Socionext (Japan)
2015-2023
Takeda (United States)
2021-2023
East Japan Railway (Japan)
2023
Osaka University
1985-2021
Ube Frontier University
2014-2021
Kyoto Institute of Technology
2017-2020
Kyoto University
1999-2019
Live fluorescence microscopy has the unique capability to probe dynamic processes, linking molecular components and their localization with function. A key goal of is increase spatial temporal resolution while simultaneously permitting identification multiple specific components. We demonstrate a new microscope platform, OMX, that enables subsecond, multicolor four-dimensional data acquisition also provides access subdiffraction structured illumination imaging. Using this platform image...
Ring-contracted porphyrins: meso-Aryl subporphyrins were synthesized by using a method in which tripyrrolylborane acts as template for the Adler method. This study demonstrates that, although retain porphyrinic properties, ring contraction has significant impact on electronic structure. The picture shows DFT-optimized structure of meso-phenyl subporphyrin (B yellow, N blue, O red, C green, H white). Supporting information this article is available WWW under...
Significance The high-mobility group box 1 (HMGB1) protein is abundantly expressed in the nucleus where it regulates chromatin function. More recently, was found to also function cytoplasm and extracellular milieu for regulation of immunity inflammation. However, vivo study HMGB1 has been hampered by fact that HMGB1-deficient mice die soon after birth. In this study, we successfully generated Hmgb1 -floxed achieve conditional inactivation gene a cell- tissue-specific manner. We demonstrate...
The synthesis of several meso-triaryl-subporphyrins, based on utilizing tripyrrolylborane as a precursor in reaction with arylaldehydes (where aryl = phenyl, 4- and 3-pyridyl, tolyl, 4-methoxyphenyl, 4-(trifluoromethyl)phenyl) under reflux propionic acid, is reported. All the compounds have been successfully characterized by 1H−, 13C−, 13C−1H 2D NMR, electronic absorption, magnetic circular dichroism (MCD), IR, fluorescence spectroscopy, together cyclic (CV) differential pulse (DPV)...
CD4+ T cells induced from human iPSCs (iCD4+ cells) offer a therapeutic opportunity for overcoming immune pathologies arising hematopoietic stem cell transplantation. However, most iCD4+ are conventional helper cells, which secrete inflammatory cytokines. We high-level expression of FOXP3, master transcription factor regulatory in cells. Human iPSC-derived, FOXP3-induced Treg-like) did not cytokines upon activation. Moreover, they showed demethylation the Treg-specific region, suggesting...
Photoactivated localization microscopy (PALM) and related fluorescent biological imaging methods are capable of providing very high spatial resolutions (up to 20 nm). Two major demands limit its widespread use on samples: requirements for photoactivatable/photoconvertible molecules, which sometimes difficult incorporate, background signals from autofluorescence or fluorophores in adjacent focal planes three-dimensional reduces PALM resolution significantly. We present here a high-resolution...
Correction of chromatic shift is necessary for precise registration multicolor fluorescence images biological specimens. New emerging technologies in microscopy with increasing spatial resolution and penetration depth have prompted the need more accurate methods to correct aberration. However, amount region interest samples often deviates from theoretical prediction because unknown dispersion samples. To measure samples, we developed a quadrisection phase correlation approach computationally...
Abstract It is generally believed that silent chromatin condensed and transcriptionally active decondensed. However, little known about the relationship between condensation levels gene expression. Here we report of interphase in fission yeast Schizosaccharomyces pombe examined by super-resolution fluorescence microscopy. Unexpectedly, less than euchromatin. Furthermore, telomeric regions are flanked highly bodies, or ‘knobs’. Knob span ∼50 kb sequence devoid methylated histones. independent...
With the rapid growth of data traffic in centers, rates over 50Gb/s/signal (e.g., OIF-CEI-56G-VSR) will eventually be required wireline chip-to-module or chip-to-chip communications [1-3]. To achieve better power efficiency than that existing 25Gb/s/signal designs, a high-speed yet energy-efficient front-end is needed both transmitter and receiver. A receiver with baud-rate architecture [1] has been successfully operated at 56Gb/s, but additional components such as eye-monitoring...
Inner nuclear membrane (INM) proteins play a role in spatial organization of chromosomes within the nucleus. In fission yeast Schizosaccharomyces pombe, Sad1, an INM protein conserved SUN-domain family, plays active moving along membranes during meiotic prophase. Ima1 is another recently identified. A previous study claimed that essential for mitotic cell growth, linking centromeric heterochromatin to spindle-pole body. However, we obtained results contradictory previously proposed Ima1: was...
During the sexual cycle of ciliate Paramecium, assembly somatic genome includes precise excision tens thousands short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these rearrangements are initiated introduction developmentally programmed DNA double-strand breaks (DSBs), which depend on domesticated transposase PiggyMac. These DSBs all exhibit a characteristic geometry, with 4-base 5′...
Chromosome structure is dramatically altered upon entering meiosis to establish chromosomal architectures necessary for the successful progression of meiosis-specific events. An early meiotic event involves replacement non-SMC mitotic cohesins with their equivalents in most part chromosome, forming an axis on chromosomes. We previously demonstrated that cohesin complex required chromosome compaction during prophase fission yeast Schizosaccharomyces pombe. These studies revealed chromosomes...
Abstract A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying formation chromatin structure function subtelomeres, telomere-adjacent regions, remain obscure. Here we report roles conserved centromeric protein Shugoshin 2 (Sgo2) in defining functions subtelomeres fission yeast Schizosaccharomyces pombe . We show that Sgo2 localizes at preferentially during G phase essential for a highly condensed subtelomeric body ‘knob’....
Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation, increased genomic instability replicative stress, defective homology-dependent DNA damage repair. Here show surprisingly distinct phenotypes result from G34V (also glioma) G34W (giant cell tumors...
Oncology therapies targeting the immune system have improved patient outcomes across a wide range of tumor types, but resistance due to an inadequate T-cell response in suppressive microenvironment (TME) remains significant problem. New that activate innate and relieve this suppression may be beneficial overcome hurdle. TAK-676 is synthetic novel stimulator interferon genes (STING) agonist designed for intravenous administration. Here we demonstrate dose-dependently triggers activation STING...
Ringverengte Porphyrine: Die Titelverbindungen wurden auf einem Weg erhalten, bei dem Tripyrrolylboran als Templat für die Adler-Methode fungiert. Untersuchung belegt, dass Subporphyrine zwar einige Porphyrineigenschaften behalten, Ringverengung aber einen erheblichen Einfluss elektronische Struktur hat. Im Bild ist DFT-optimierte des meso-Phenylsubporphyrins zu sehen (B gelb, N blau, O rot, C grün, H weiß). Supporting information for this article is available on the WWW under...
Abstract Chromosome higher order structure has been an enigma for over a century. The most important structural finding the presence of chromosome scaffold composed non-histone proteins; so-called proteins. However, organization and function are still controversial. Here, we use three dimensional-structured illumination microscopy (3D-SIM) focused ion beam/scanning electron (FIB/SEM) to reveal axial distributions proteins in metaphase chromosomes comprising two strands. We also find that...