A5058948488- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Cellular transport and secretion
- Chemical Reactions and Isotopes
- Bioactive Compounds and Antitumor Agents
- Epigenetics and DNA Methylation
- 14-3-3 protein interactions
- Amino Acid Enzymes and Metabolism
- Cancer therapeutics and mechanisms
- Protist diversity and phylogeny
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2018
Universidade do Porto
2007-2018
Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde
2014
Cooperativa de Ensino Superior Politécnico e Universitário
2011-2014
The molecular motor dynein concentrates at the kinetochore region of mitotic chromosomes in animals to accelerate spindle microtubule capture and control checkpoint signaling. In this study, we describe mechanism used by Rod–Zw10–Zwilch complex adaptor Spindly recruit kinetochores Caenorhabditis elegans embryos human cells. We show that Rod’s N-terminal β-propeller associated Zwilch subunit bind Spindly’s C-terminal domain, identify a specific mutant abrogates recruitment vivo binding Rod...
The clinical interest in farnesyltransferase inhibitors (FTIs) makes it important to understand how these compounds affect cellular processes involving farnesylated proteins. Mitotic abnormalities observed after treatment with FTIs have so far been attributed defects the farnesylation of outer kinetochore proteins CENP-E and CENP-F, which are involved chromosome congression spindle assembly checkpoint signaling. Here we identify cytoplasmic dynein adaptor Spindly as an additional component...
Abstract An important group of microtubule associated proteins are the plus‐end tracking which includes Mast/Orbit/CLASPs family amongst others. Several these have functions during interphase and mitosis in modulation dynamic properties microtubules, however, precise mechanism remains to be elucidated. To investigate role Mast regulation behaviour interphase, we used RNAi Drosophila S2 culture cells stably expressing GFP‐α‐tubulin followed microtubules vivo. depleted show a significant...
A predominant mechanism of spindle assembly checkpoint (SAC) silencing is dynein-mediated transport certain kinetochore proteins along microtubules. There are still conflicting data as to which SAC dynein cargoes. Using two ATP reduction assays, we found that the core Mad1, Mad2, Bub1, BubR1, and Bub3 redistributed from attached kinetochores poles, in a dynein-dependent manner. This redistribution occurred metaphase-arrested cells, at time when should be satisfied silenced. Unexpectedly,...
Establishment and maintenance of the mitotic spindle requires balanced activity microtubule-associated proteins motors. In this study we have addressed how microtubule plus-end tracking protein Mast/Orbit/CLASP cytoplasmic dynein regulate process in Drosophila melanogaster embryos S2 cells. We show that Mast accumulates at kinetochores early mitosis, which is followed by a poleward streaming upon attachment. This leads to reduction levels during metaphase anaphase depends largely on minus...
Single nucleotide polymorphisms were examined in the cytochrome 450 3A4 ( CYP3A4 ) and N -acetyltransferase 2 NAT2 genes, which code for major mediators of metabolism a wide variety therapeutic drugs, as well xenobiotics. We determined, population from Guinea-Bissau, frequencies variants expected to be prevalent among Africans, due high frequency previously observed African Americans. The 72% CYP3A4*1B 19.2% 191 G>A variant. found these potentially function-altering suggests possibility...
SUMMARY The kinetochore is a dynamic multi-protein assembly that forms on each sister chromatid and interacts with microtubules of the mitotic spindle to drive chromosome segregation. In animals, kinetochores without attached expand their outermost layer into crescent ring shapes promote microtubule capture checkpoint (SAC) signalling. Kinetochore expansion an example protein co-polymerization, but mechanism not understood. Here, we present evidence driven by oligomerization Rod-Zw10-Zwilch...