A5083621146- Neuroscience and Neuropharmacology Research
- Forensic Toxicology and Drug Analysis
- Microtubule and mitosis dynamics
- Neurotransmitter Receptor Influence on Behavior
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Drug Transport and Resistance Mechanisms
- Mitochondrial Function and Pathology
- Psychedelics and Drug Studies
- Cannabis and Cannabinoid Research
- Paraquat toxicity studies and treatments
- Tryptophan and brain disorders
- Metabolomics and Mass Spectrometry Studies
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Protein Interaction Studies and Fluorescence Analysis
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Natural Compound Pharmacology Studies
- Forensic Entomology and Diptera Studies
- Carcinogens and Genotoxicity Assessment
- Pharmacological Effects and Toxicity Studies
- Barrier Structure and Function Studies
- Chemical synthesis and alkaloids
- RNA modifications and cancer
Cooperativa de Ensino Superior Politécnico e Universitário
2022-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2025
Unidade em Ciências Biomoleculares Aplicadas
2024-2025
Universidade do Porto
2013-2024
Instituto de Biologia Molecular e Celular
2015-2023
Rede de Química e Tecnologia
2010-2018
INESC TEC
2018
In-Q-Tel
2015
Universitat de Barcelona
2013-2014
Biomedical Research Networking Center on Neurodegenerative Diseases
2013-2014
The molecular motor dynein concentrates at the kinetochore region of mitotic chromosomes in animals to accelerate spindle microtubule capture and control checkpoint signaling. In this study, we describe mechanism used by Rod–Zw10–Zwilch complex adaptor Spindly recruit kinetochores Caenorhabditis elegans embryos human cells. We show that Rod’s N-terminal β-propeller associated Zwilch subunit bind Spindly’s C-terminal domain, identify a specific mutant abrogates recruitment vivo binding Rod...
The MAP kinase and motor scaffold JIP3 prevents excess lysosome accumulation in axons of vertebrates invertebrates. How JIP3's interaction with dynein kinesin-1 contributes to organelle clearance is unclear. We show that human light intermediate chain (DLIC) binds the N-terminal RH1 domain JIP3, its paralog JIP4, lysosomal adaptor RILP. A point mutation abrogates DLIC binding without perturbing between kinesin heavy chain. Characterization this separation-of-function Caenorhabditis elegans...
All animal cells use the motor cytoplasmic dynein 1 (dynein) to transport diverse cargo toward microtubule minus ends and organize position arrays such as mitotic spindle. Cargo-specific adaptors engage with recruit activate motor, but molecular mechanisms remain incompletely understood. Here, we structural dynamic nuclear magnetic resonance (NMR) analysis demonstrate that C-terminal region of human light intermediate chain (LIC1) is intrinsically disordered contains two short conserved...
Drug abuse represents a significant public health problem with growing tendency. As way of circumventing the strict national and international control psychoactive substances by regulatory agencies, there is market release new activity, called New Psychoactive Substances (NPSs). This group encompasses diverse range synthetic compounds designed to mimic effects traditional illicit substances. NPSs show stronger than classical drugs, they pose unique challenges frameworks. Additionally, some...
The biological barriers existing in the human body separate blood circulation from interstitial fluid tissues. blood-brain barrier (BBB) isolates central nervous system bloodstream, presenting a dual role: protection of brain against potentially toxic/harmful substances coming blood, while providing nutrients to and removing metabolites. In terms architectural features, presence junctional proteins (that restrict paracellular transport) existence efflux transporters at BBB are two major vivo...
Drug abuse presents a significant global health challenge as the illicit drug market progresses from classic drugs to growing prevalence of New Psychoactive Substances (NPS), particularly synthetic cathinones, which, although illegal, are often falsely marketed safe and legal alternatives. The rapid increase in use these complicates assessment their safety effects on human health. However, they pose unique toxicological concerns that remain largely uncharacterized. This study investigated...
Designer drugs like 2C-I and 25I-NBOMe have emerged as potent psychoactive substances, with several reports linking their consumption to severe poisoning fatalities. However, there is limited information on toxicity, particularly in vivo models. In this manuscript, we evaluate the survival, developmental, reproductive impact of these designer model organism Caenorhabditis elegans (C. elegans). For purpose, adult worms synchronized at L1 stage were exposed growing concentrations 25I-NBOMe....
BACKGROUND AND PURPOSE 3,4‐Methylenedioxymethamphetamine (MDMA or ‘Ecstasy’) is a worldwide major drug of abuse known to elicit neurotoxic effects. The mechanisms underlying the effects MDMA are not clear at present, but metabolism dopamine and 5‐HT by monoamine oxidase (MAO), as well hepatic biotransformation into pro‐oxidant reactive metabolites thought contribute its adverse EXPERIMENTAL APPROACH Using mouse brain synaptosomes, we evaluated metabolites, α‐methyldopamine (α‐MeDA),...
Abuse of synthetic drugs is widespread among young people worldwide. In this context, piperazine derived recently appeared in the recreational drug market. Clinical studies and case-reports describe sympathomimetic effects including hypertension, tachycardia, increased heart rate. Our aim was to investigate cytotoxicity N-benzylpiperazine (BZP), 1-(3-trifluoromethylphenyl) (TFMPP), 1-(4-methoxyphenyl) (MeOPP), 1-(3,4-methylenedioxybenzyl) (MDBP) H9c2 rat cardiac cell line. Complete curves...
The nervous system has a highly complex organization, including many cell types with multiple functions, an intricate anatomy and unique structural functional characteristics; the study of its (dys)functionality following exposure to xenobiotics, neurotoxicology, constitutes important issue in neurosciences.
The microtubule-based motor dynein generates pulling forces for centrosome centration and mitotic spindle positioning in animal cells. How the essential activator dynactin regulates these functions of is incompletely understood. Here, we dissect role dynactin's microtubule binding activity, located p150 CAP-Gly domain an adjacent basic patch, C. elegans zygote. Analysis mutants engineered by genome editing suggests that tip tracking dynein-dynactin dispensable targeting to cell cortex...