A5059967290- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Physics of Superconductivity and Magnetism
- DNA and Nucleic Acid Chemistry
- Single-cell and spatial transcriptomics
- Virus-based gene therapy research
- Quantum and electron transport phenomena
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- Animal Virus Infections Studies
- Social and Educational Sciences
- Lipid Membrane Structure and Behavior
- AI in cancer detection
- Advanced Fluorescence Microscopy Techniques
- Iron-based superconductors research
- Evaluation and Performance Assessment
- Viral Infections and Immunology Research
- Topological Materials and Phenomena
- Mathematics, Computing, and Information Processing
- Surface and Thin Film Phenomena
- Advanced Malware Detection Techniques
- Water Systems and Optimization
- Corporate Finance and Governance
- Superconductivity in MgB2 and Alloys
- Cholangiocarcinoma and Gallbladder Cancer Studies
Oregon Health & Science University
2021-2022
Chalmers University of Technology
2000-2016
Ludwig-Maximilians-Universität München
2006
Highly multiplexed tissue imaging makes detailed molecular analysis of single cells possible in a preserved spatial context. However, reproducible large multichannel images poses substantial computational challenge. Here, we describe modular and open-source pipeline, MCMICRO, for performing the sequential steps needed to transform whole-slide into single-cell data. We demonstrate use MCMICRO on tumor acquired using multiple platforms, thereby providing solid foundation continued development software.
The potential use of polypeptides and oligonucleotides for therapeutical purposes has been questioned because their inherently poor cellular uptake. However, the 16‐mer oligopeptide penetratin, derived from homeodomain Antennapedia, reported to enter cells readily via a non‐endocytotic receptor‐ transporter‐independent pathway, even when conjugated large hydrophilic molecules. We here present first study where penetratin is shown traverse pure lipid bilayer. results support idea that uptake...
Cell-penetrating peptides (CPPs) have been extensively studied during the past decade, because of their ability to promote cellular uptake various cargo molecules, e.g., oligonucleotides and proteins. In a recent study several analogues penetratin, Tat(48−60) oligoarginine in live (unfixed) cells [Thorén et al. (2003) Biochem. Biophys. Res. Commun. 307, 100−107], it was found that both endocytotic nonendocytotic pathways are involved internalization these CPPs. present study, membrane...
We present an extensive experimental and theoretical study of the proximity effect in InAs nanowires connected to superconducting electrodes. fabricate investigate devices with suspended gate-controlled nonsuspended nanowires, a broad range lengths normal-state resistances. analyze main features current-voltage characteristics: Josephson current, excess subgap current as functions length, temperature, magnetic field, gate voltage, compare them theory. The critical for short-length device, L...
The binding of penetratin, a peptide that has been found useful for cellular delivery large hydrophilic molecules, to negatively charged vesicles was investigated. surface charge density the varied by mixing zwitterionic dioleoylphosphatidylcholine (DOPC) and dioleoylphosphatidylglycerol (DOPG) at various molar ratios. extent membrane association quantified from tryptophan emission spectra recorded during titration solution with liposomes. A singular value decomposition spectral data...
The interaction of the cellular delivery vector penetratin with a model system consisting negatively charged phospholipid vesicles has been studied. Above certain peptide to lipid molar ratio, cationic oligopeptide induces vesicle aggregation. Interestingly, aggregation is followed by spontaneous disaggregation, which may be related membrane translocation peptide. Circular dichroism (CD) measurements indicate conformational transition, from α‐helix antiparallel β‐pleated sheet, simultaneous...
We report conductance and supercurrent of InAs nanowires coupled to Al-superconducting electrodes with short channel lengths good Ohmic contacts. The are suspended 15 nm above a local gate electrode. charge density in the can be controlled by small change voltage. For large negative voltages, number conducting channels is reduced gradually, we observe stepwise decrease both critical current before vanishes completely.
Reports on serious artifacts associated with the use of cell fixation in studies cellular uptake cell-penetrating peptides, also denoted protein transduction domains, have demonstrated need for a reevaluation current understanding peptide-mediated delivery large, hydrophilic molecules. In recent study internalization unfixed cells penetratin and its analogues which tryptophans are substituted phenylalanines (Pen2W2F), lysines arginines (PenArg), (PenLys), we revealed large dissimilarities...
Conventional superconductors disordered by magnetic impurities demonstrate physical properties that are drastically different from their pristine counterparts. In our previous work [D. Persson et al., Phys. Rev. B 92, 245430 (2015)], we explored the spectral and thermodynamic of such systems for two extreme cases: completely random ferromagnetically aligned impurity moments. Here consider transport these show they have a potential to be used in superconducting spintronic devices. Each...
Abstract Highly multiplexed tissue imaging (MTI) are powerful spatial proteomics technologies that enable in situ single-cell characterization of tissues. However, analysis and visualization MTI datasets remains challenging, we developed the Galaxy-ME software hub to address this challenge.Galaxy-ME is a web-based, interactive enables end-to-end accessible everyone. To demonstrate its utility, was used analyze obtained from multiple assays evaluate assay concordance both normal cancerous...
We present a comprehensive theoretical study of thermodynamic properties superconductors with dilute concentration magnetic impurities, focus on how the superconducting host change if moments impurities order ferromagnetically. Scattering off leads to formation band Yu-Shiba-Rusinov states within energy gap that drastically influences superconductivity. In magnetically ordered system, magnetization displays sudden drop as function impurity density or moment amplitude. The occurs...
Abstract Paint formulations are complex mixtures of both dissolved and particulate components, organic inorganic. Amorphous silica nanoparticles included to make the paint layer dirt-repellent. Over entire life cycle paints containing such nanoparticles, however, their multicomponent composition may raise several concerns as hazard when exposed humans environment, notably due potential release incorporated nano-objects. To mitigate concerns, Safe-by-Design target specifications can be...