A5044906070- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Physiological and biochemical adaptations
Nanjing Agricultural University
2016-2017
Pheromone binding proteins (PBPs) are thought to play crucial roles in perception of the sex pheromones particularly noctuid moths, but this is rarely vivo evidenced due lacking an effective technique. Here, we reported functional study PBP1 important lepidopteran pest Helicoverpa armigera (HarmPBP1), by using CRISPR/Cas9 system. Efficient and heritable mutagenesis was achieved egg injection mixture Cas9-mRNA HarmPBP1-sgRNA. The TA cloning sequencing revealed various insertion and/or...
Pheromone-binding proteins (PBPs) are thought to bind and transport sex pheromones onto the olfactory receptors on dendrite membrane of neurons, thus play a vital role in pheromone perception. However, function PBPs has rarely been demonstrated vivo. In this study, two (PBP1 PBP3) Chilo suppressalis, one most notorious pyralid pests, were vivo functionally characterized using insects with PBP gene knocked out by CRISPR/Cas9 system. First, through direct injection PBP-single guide RNA...