A5066514643- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Insect-Plant Interactions and Control
- Insect Pest Control Strategies
- Animal Behavior and Reproduction
- Plant Virus Research Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hemiptera Insect Studies
- RNA and protein synthesis mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Hematopoietic Stem Cell Transplantation
- Insect and Pesticide Research
- Physiological and biochemical adaptations
- Silkworms and Sericulture Research
- Immune responses and vaccinations
- Plant biochemistry and biosynthesis
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
University of Kentucky
2019-2024
Sun Yat-sen University
2021-2024
St. Michael's Hospital
2023
Nanjing Agricultural University
2016-2020
RNAi is a potent technique for the knockdown of target genes. However, its potential off-target effects limit widespread applications in both reverse genetic analysis and manipulation. Previous efforts have uncovered rules underlying specificity siRNA-based silencing, which has broad humans, but basis dsRNAs, are better suited use as insecticides, poorly understood. Here, we investigated governing dsRNA specificity. Mutational analyses showed that dsRNAs with >80% sequence identity genes...
The yellow fever mosquito, Aedes aegypti, vectors human pathogens. Juvenile hormones (JH) control almost every aspect of an insect's life, and JH analogs are currently used to mosquito larvae. Since RNA interference does not work efficiently during the larval stages this insect, regulation development mode action well studied. To overcome limitation, we a multiple single guide RNA-based CRISPR/Cas9 genome-editing method knockout methoprene-tolerant (Met) gene coding for receptor. Met larvae...
Pheromone binding proteins (PBPs) are thought to play crucial roles in perception of the sex pheromones particularly noctuid moths, but this is rarely vivo evidenced due lacking an effective technique. Here, we reported functional study PBP1 important lepidopteran pest Helicoverpa armigera (HarmPBP1), by using CRISPR/Cas9 system. Efficient and heritable mutagenesis was achieved egg injection mixture Cas9-mRNA HarmPBP1-sgRNA. The TA cloning sequencing revealed various insertion and/or...
Pheromone-binding proteins (PBPs) are thought to bind and transport sex pheromones onto the olfactory receptors on dendrite membrane of neurons, thus play a vital role in pheromone perception. However, function PBPs has rarely been demonstrated vivo. In this study, two (PBP1 PBP3) Chilo suppressalis, one most notorious pyralid pests, were vivo functionally characterized using insects with PBP gene knocked out by CRISPR/Cas9 system. First, through direct injection PBP-single guide RNA...
Azadirachtin has been used to control agricultural pests for a long time; however, the molecular mechanism of azadirachtin on lepidopterans is still not clear. In this study, fourth instar larvae fall armyworm were fed with azadirachtin, and then ecdysis was blocked in larval stage (L4). The prothoracic glands (PGs) treated dissected RNA sequencing determine effect inhibition. Interestingly, one PG-enriched genes, nuclear hormone receptor 3 (HR3), decreased after treatment, which plays...
Insect courtship and mating depend on integration of olfactory, visual, tactile cues. Compared to other insects, Bombyx mori, the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas9 electrophysiological techniques we found that are regulated in male silk moths mutating genes sex determination cascade belonging two conserved pathways. Loss Bmdsx gene expression significantly reduced peripheral perception major pheromone component bombykol...
Double-stranded RNA (dsRNA), the unique trigger of interference, could be used as potential pesticides for management storage insects. High species specificity greatly improves biosafety dsRNAs. However, there are usually more than one insect in real circumstances. In this study, we present a new strategy that broadens control spectrum formulation using single dsRNA fragments. First, effective target genes were selected each pest, here including Rhyzopertha dominica and Blattella germanica....
Azadirachtin is a widely used botanical pesticide for agricultural pest control worldwide. However, the molecular mechanisms of azadirachtin in insects are not fully understood. In this study, histological analysis and RNA sequencing were conducted to investigate impact on larval development Spodoptera frugiperda. Under exposure, was completely inhibited, major internal tissues, fat body, midgut strongly damaged under analysis. Differential gene expression demonstrated that nutrient...
The high sensitivity of the olfactory system is essential for feeding and oviposition in moth insects, some chemosensory proteins (CSPs) are thought to play roles this by binding carrying hydrophobic odorants across aqueous sensillar lymph. In study, identify CSPs from a repertoire 21 CSP members notorious rice pest Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), tissue expression patterns were firstly examined quantitative real-time polymerase chain reaction (qPCR). It showed that...
Methoprene, a juvenile hormone (JH) analog, is widely used for insect control, but its mode of action not known. To study methoprene in the yellow fever mosquito, Aedes aegypti, E93 (ecdysone-induced transcription factor) was knocked out using CRISPR-Cas9 system. The mutant pupae retained larval tissues similar to methoprene-treated insects. These insects completed pupal ecdysis and died as pupa. In addition, expression factors, broad complex Krüppel homolog 1 (Kr-h1), increased that...
Abstract Insect courtship and mating depend on integration of olfactory, visual, tactile cues. Compared to other insects, Bombyx mori , the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas electrophysiological techniques we found that are regulated in male silk moths mutating genes sex determination cascade belonging two conserved pathways. Loss Bmdsx gene expression significantly reduced peripheral perception major pheromone component...
In insects, the shedding of old exoskeleton is accomplished through ecdysis which typically followed by expansion and tanning new cuticle. Four neuropeptides, eclosion hormone (EH), triggering (ETH), crustacean cardioactive peptide (CCAP) bursicon (Bur) are known to control ecdysis. However, regulation these neuropeptide genes still poorly understood. Here, we report that in red flour beetle (RFB) Tribolium castaneum fall armyworm (FAW) Spodoptera frugiperda , knockdown or knockout SoxC gene...
Abstract BACKGROUND Holometabolan pupal‐specifier broad‐complex ( BR‐C ) and adult specifier ecdysone‐induced protein 93F E93 are essential for metamorphosis; however, their interaction effects on programmed cell death differentiation during pupation remain unclear. RESULTS Here, multiple single‐guide RNA (sgRNA)‐mediated mosaic knockout of induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda . Quantitative real‐time polymerase chain reaction (qPCR) analysis showed...
The juvenile hormone (JH) and 20-hydroxyecdysone (20E) are the central regulating hormones of insect development. timing their secretion usually leads to developmental transitions.