A5101619887- Silkworms and Sericulture Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Animal Behavior and Reproduction
- Silk-based biomaterials and applications
- Interconnection Networks and Systems
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Photocatalysis Techniques
- Sperm and Testicular Function
- Ichthyology and Marine Biology
- Parallel Computing and Optimization Techniques
- Evolution and Paleontology Studies
- Plant Virus Research Studies
- Distributed and Parallel Computing Systems
- TiO2 Photocatalysis and Solar Cells
- Copper-based nanomaterials and applications
- RNA Research and Splicing
- Paleontology and Evolutionary Biology
Chinese Academy of Sciences
2004-2020
Center for Excellence in Molecular Plant Sciences
2016-2020
Shanghai Institutes for Biological Sciences
2016-2019
University of Chinese Academy of Sciences
2016-2019
Lanzhou Institute of Chemical Physics
2015
Zoucheng People's Hospital
2014
Institute of Computing Technology
2004
Manipulation of sex determination pathways in insects provides the basis for a wide spectrum strategies to benefit agriculture and public health. Furthermore, display remarkable diversity genetic that lead differentiation. The silkworm, Bombyx mori, has been cultivated by humans as beneficial insect over two millennia, more recently model system studying lepidopteran genetics development. Previous studies have identified B. mori Fem piRNA primary female determining factor BmMasc its...
We developed a novel antiviral strategy by combining transposon-based transgenesis and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system for direct cleavage of Bombyx mori nucleopolyhedrovirus (BmNPV) genome DNA to promote virus clearance in silkworms. demonstrate that transgenic silkworms constitutively expressing Cas9 guide RNAs targeting BmNPV immediate early-1 (ie-1) me53 genes effectively induce target-specific subsequent...
Abstract Sperm, which have a vital role in sexual reproduction the animal kingdom, can display heteromorphism some species. The regulation of sperm dichotomy remains longstanding puzzle even though phenomenon has been widely documented for over century. Here we use Bombyx mori as model to study form dimorphism (eupyrene and apyrene sperm), is nearly universal among Lepidoptera. We demonstrate that B. Sex-lethal ( BmSxl ) crucial development, poly(A)-specific ribonuclease-like...
Identification of stage- and tissue-specific cis-regulatory elements will enable more precise genomic editing. In previous studies the silkworm Bombyx mori, we identified characterized several tissue- sex-specific using transgenic technology, including a female- fat body-specific promoter, vitellogenin, testis-specific promoters, Radial spoke head 1 (BmR1) beta-tubulin 4 (Bmβ4). Here report element specific for somatic germ cell-expressed nanos (Bmnos). We investigated activities three...
The close affinity between Edmontosaurus and Shantungosaurus is corroborated on the basis of following shared features: an occipital condyle deflected strongly posteroventrally; a posterodorsally reflected, lip-shaped oral margin premaxilla, with deep, oval concavity at anteromedial corner bone; well-demarcated posterodorsal deeply excavated circumnarial fossa formed by prominent arched ridge along entire posterior half lateroventral border nasal; anteroposteriorly broad jugal process...
Insect courtship and mating depend on integration of olfactory, visual, tactile cues. Compared to other insects, Bombyx mori, the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas9 electrophysiological techniques we found that are regulated in male silk moths mutating genes sex determination cascade belonging two conserved pathways. Loss Bmdsx gene expression significantly reduced peripheral perception major pheromone component bombykol...
MicroRNAs (miRNAs) are a class of short, non-coding transcripts that bind to 3'-untranslated regions trigger messenger RNA degradation or translational inhibition. Here we explored how miRNAs regulate sex determination in Bombyx mori, lepidopteran model insect. Genes known be involved determination, BmPSI, Bmdsx, and BmMasc, predicted targets the species-specific miR-2738. Using dual luciferase reporter assay HEK293T cells, confirmed miR-2738 suppressed transcription BmMasc. The levels BmPSI...
A hierarchical model for parallel computations is introduced and evaluated in this paper. This describes the general homogeneous computer systems with Hierarchical Parallelism Memories (named as HPM). The HPM consists of a hierarchy ERAMs that cooperate each other. function HP multi-level parallelism system. memory Hm shows characteristics memories. organisation implementing accesses or data communication system known by joint name: generalised binding relation HB gives connecting defines...
Abstract Insect courtship and mating depend on integration of olfactory, visual, tactile cues. Compared to other insects, Bombyx mori , the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas electrophysiological techniques we found that are regulated in male silk moths mutating genes sex determination cascade belonging two conserved pathways. Loss Bmdsx gene expression significantly reduced peripheral perception major pheromone component...