A5100721706- Neurobiology and Insect Physiology Research
- Silkworms and Sericulture Research
- Insect Resistance and Genetics
- RNA modifications and cancer
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Insect and Arachnid Ecology and Behavior
- Ubiquitin and proteasome pathways
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Regulation of Appetite and Obesity
- Diet and metabolism studies
- Silk-based biomaterials and applications
- TGF-β signaling in diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Axon Guidance and Neuronal Signaling
- Psoriasis: Treatment and Pathogenesis
- Signaling Pathways in Disease
- Orthopaedic implants and arthroplasty
- Respiratory Support and Mechanisms
- Angiogenesis and VEGF in Cancer
First Affiliated Hospital of Shantou University Medical College
2015-2025
Central South University
2005-2025
Second Xiangya Hospital of Central South University
2005-2025
Hunan Provincial Center for Disease Control and Prevention
2025
Fujian Medical University
2009-2025
Sir Run Run Shaw Hospital
2025
Zhejiang University
2025
Wenzhou Medical University
2020-2025
Zhejiang Taizhou Hospital
2020-2025
Fujian Provincial Cancer Hospital
2025
Significance Hematophagous female mosquitoes transmit devastating human diseases. Owing to their obligatory blood feeding, they require an extremely high level of lipid metabolism for reproduction. We found that microRNA-277 (miR-277) plays a critical role in Aedes aegypti mosquitoes. The genetic disruption miR-277 using the CRISPR-Cas9 system showed impairment both storage and ovarian development. Insulin/FOXO signaling was up-regulated after depletion. Comprehensive screening functional...
Significance Mosquitoes pose an enormous threat to humans by transmitting numerous dangerous diseases. To become reproductively competent and effective disease vectors, it is crucial for them achieve optimal body size nutritional status. Elucidation of these processes in mosquitoes has been hampered the lack classic genetics tools. CRISPR-Cas9 disruption fat-body–specific serotonin receptor Aa5HT2B impairs growth lipid accumulation Aedes aegypti mosquitoes, vectors dengue fever, yellow Zika...
Significance In hematophagous female mosquitoes, each reproductive cycle is linked to a separate blood intake, serving as foundation for the transmission of dangerous human diseases. During cycle, mosquitoes sequentially feed on carbohydrates and protein (blood). Metabolic flux alternated support cyclicity. We have established that insulin-like peptides (ILPs), critical regulating metabolism, are genetically controlled by juvenile hormone (JH) 20-hydroxyecdysone (20E), key hormones governing...
Hypocretins (HCRT-1 and HCRT-2), also known as orexins, are neuropeptides localized in neurons surrounding the perifornical region of posterior hypothalamus. These project to major arousal centers brain implicated regulating wakefulness. In young rats monkeys, levels HCRT-1 highest at end wake-active period lowest toward sleep period. However, effects age on diurnal rhythm not known. To provide such data, cerebrospinal fluid (CSF) was collected from cisterna magna (2-month-old, n = 9),...
Differentiation of cardiac fibroblasts into myofibroblasts is a critical event in the progression fibrosis that leads to pathological remodeling. Metformin, an antidiabetic agent, exhibits number cardioprotective properties. However, much less known regarding effect metformin on fibroblast differentiation. Thus, present study, we examined angiotensin (Ang) II-induced differentiation and its underlying mechanism. Adult rat were stimulated with Ang II (100 nM) presence or absence (10–200 µM)....
MicroRNAs (miRNAs) are post-transcriptional regulators that target specific mRNAs for repression and thus play key roles in many biological processes, including insect wing morphogenesis. miR-2 is an invertebrate-specific miRNA family has been predicted the fruit fly, Drosophila melanogaster, to be involved regulating Notch signaling pathway. We show here plays a critical role morphogenesis silkworm, Bombyx mori, lepidopteran model insect. Transgenic over-expression of cluster using Gal4/UAS...
Genome-wide association studies (GWAS) have identified >500 common variants associated with quantitative metabolic traits, but in aggregate such explain at most 20–30% of the heritable component population variation these traits. To further investigate impact genotypic on we conducted re-sequencing >6,000 members a Finnish cohort (The Northern Finland Birth Cohort 1966 [NFBC]) and type 2 diabetes case-control sample Finland-United States Investigation NIDDM Genetics [FUSION] study). By...
The Global Initiative for Chronic Obstructive Lung Disease (GOLD) 2023 revised the combined chronic obstructive pulmonary disease (COPD) assessment, merging groups C and D into group E, initial inhalation therapy recommendation. We aimed to evaluate treatment responses among different therapies in GOLD E patients stratified by COPD assessment test (CAT) scores forced expiratory volume one-second percentage of predicted (FEV1%pred). In this retrospective cohort study, we included with...
We aimed to validate the performance of ddPCR-GNB, a plasma droplet digital PCR panel targeting four most common gram-negative bacteria, for patients with suspected bloodstream infection (BSI). Patients having BSIs were prospectively enrolled. The results blood culture and ddPCR-GNB compared, cases discordant arbitrated on basis additional microbiological clinical evidence. A total 1,041 Blood positive targeted bacteria in 6.3% 31.7% patients, respectively. overall per-patient sensitivity...
Background: A growing number of patients with early-stage cervical cancer opt for local tumor resection to preserve the uterus. Nevertheless, there is still a dearth long-term comparisons between destruction/excision (LTD/LTE) and total hysterectomy(TH), optimal target population excision remains uncertain. Methods: multicenter retrospective study utilizing Surveillance, Epidemiology, End Results (SEER) database compare outcomes LTD/LTE TH in stage IA was conducted. 1:1 propensity score...
Introduction Pulmonary fibrosis (PF) is a fatal pathological subtype of interstitial lung disease, frequently manifests as pulmonary complication connective tissue disease. Iguratimod (IGU) new class anti-rheumatic drugs used in the treatment rheumatoid arthritis (RA). Studies have reported that RA patients treated with IGU better function, and effectively ameliorates PF. However, mechanism by which improves PF still unclear. This study aims to elucidate therapeutic efficacy mechanisms...
Background Although the clinical outcome of acute myocardial infarction (AMI) in patients with type 2 diabetes mellitus (T2DM) is well established to be worse than for non-diabetic patients, reasons this remain unclear. We hypothesized that may related impairment bone marrow-derived endothelial progenitor cells (EPCs) mobilization. Methodology/Principal Findings observed short term marrow EPCs mobilization and long outcomes 62 AMI or without T2DM investigated levels as pathway changes a rat...
Metamorphosis in insects includes a series of programmed tissue histolysis and remolding processes that are controlled by two major classes hormones, juvenile hormones ecdysteroids. Precise pulses ecdysteroids (the most active ecdysteroid is 20-hydroxyecdysone, 20E), regulated both biosynthesis metabolism. In this study, we show ecdysone oxidase (EO), 20E inactivation enzyme, expresses predominantly the midgut during early pupal stage lepidopteran model insect, Bombyx mori . Depletion BmEO...
Abstract Engineering sex‐specific sterility is critical for developing transgene‐based sterile insect technology. Targeted genome engineering achieved by customized zinc‐finger nuclease, transcription activator‐like effector nuclease ( TALEN) or clustered, regularly interspaced, short palindromic repeats/Cas9 systems has been exploited extensively in a variety of model organisms; however, screening mutated individuals without detectable phenotype still challenging. In addition, genetically...
RNA interference has been described as a powerful genetic tool for gene functional analysis and promising approach pest management. However, RNAi efficiency varies significantly among insect species due to distinct machineries. Lepidopteran insects include large number of pests well model insects, such the silkworm, Bombyx mori. only limited success in vivo reported lepidoptera, particularly during larval stages when worms feed most do harm host plant. Enhancing lepidoptera is urgently...
Psoriasis is a dermatosis with the major clinical symptoms of scale, erythema and itching, it has long disease course. In addition, easily recurrent refractory, greatly affecting physical mental health patients. present study, was hypothesized that function miR‑155 increases psoriasis‑induced inflammation its expression may be dependent on inflammasome activation. examined by gene chip array quantitative polymerase chain reaction analysis. levels were significantly increased in an vivo model...
female mosquitoes require vertebrate blood for their egg production and consequently they become vectors of devastating human diseases. Amino acids (AAs) nutrients originating from a meal activate vitellogenesis fuel embryo development anautogenous mosquitoes. Insulin-like peptides (ILPs) are indispensable in reproducing mosquitoes, regulating glycogen lipid metabolism, other essential functions. However, how ILPs coordinate action response to the AA influx mosquito reproduction was unknown....
The yellow fever mosquito Aedes aegypti is the major vector of arboviruses, causing numerous devastating human diseases, such as dengue and fevers, Chikungunya Zika. Female mosquitoes need vertebrate blood for egg development, repeated cycles feeding are tightly linked to pathogen transmission. mosquito's posterior midgut (gut) involved in digestion also serves an entry point pathogens. Thus, gut important tissue investigate. miRNA aae-miR-275 (miR-275) has been shown be required normal...