A5030946692- Cancer Immunotherapy and Biomarkers
- Uterine Myomas and Treatments
- Endometrial and Cervical Cancer Treatments
- Connexins and lens biology
- Endometriosis Research and Treatment
- Cervical Cancer and HPV Research
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- Reproductive System and Pregnancy
- Radiopharmaceutical Chemistry and Applications
- Radiomics and Machine Learning in Medical Imaging
- CAR-T cell therapy research
- Ovarian cancer diagnosis and treatment
- Bladder and Urothelial Cancer Treatments
- Signaling Pathways in Disease
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Heat shock proteins research
- Nicotinic Acetylcholine Receptors Study
- Estrogen and related hormone effects
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Endoplasmic Reticulum Stress and Disease
- Peptidase Inhibition and Analysis
Fujian Provincial Cancer Hospital
2014-2025
Fujian Medical University
2017-2025
Fudan University Shanghai Cancer Center
2024-2025
Songjiang District Central Hospital
2024
Huazhong University of Science and Technology
2013-2022
Tongji Hospital
2020-2022
Guiyang University
2019
SUNY Upstate Medical University
2010-2018
Shenzhen Second People's Hospital
2015
Community Health Center
2014
No combined immunotherapy and antiangiogenic therapy have been investigated in exclusively programmed death-ligand 1 (PD-L1)-positive advanced cervical cancer (CA). We the efficacy safety of sintilimab plus anlotinib as second-line or later for PD-L1-positive recurrent metastatic (R/M) CA.Patients with (Combined Positive Score ≥ 1) R/M CA who progressed after at least one prior systemic chemotherapeutic regimen could not tolerate chemotherapy were eligible phase II trial. The patients...
Tumor-infiltrating lymphocytes (TILs), especially CD8+ TILs, represent a favorable prognostic factor in high-grade serous ovarian cancer (HGSOC) and other tumor lineages. Here, we analyze the spatial heterogeneity of different TIL subtypes HGSOC. We integrated RNA sequencing, whole-genome bulk T cell receptor (TCR) as well single-cell RNA/TCR sequencing to investigate characteristics differential composition TILs across HGSOC sites. Two immune "cold" patterns are identified: (1) lesions with...
The characteristics of COVID-19 patients with persistent SARS-CoV-2 infection are not yet well described. Here, we compare the clinical and molecular features long duration viral shedding (LDs) those from short (SDs), healthy donors (HDs). We find that several cytokines chemokines, such as interleukin (IL)-2, tumor necrosis factor (TNF) lymphotoxin α (LT-α) present at lower levels in LDs than SDs. Single-cell RNA sequencing shows natural killer (NK) cells CD14+ monocytes reduced, while...
Context: Asoprisnil, a selective progesterone (P4) receptor (PR) modulator (SPRM) with mixed P4 agonist/antagonist activities, reduces uterine leiomyoma volume in dose-dependent manner the presence of follicular phase estrogen concentrations. The evidence from clinical studies suggests that asoprisnil may directly target leiomyomata. Objective and Methods: present study evaluated effects on cell proliferation, expression apoptosis-related proteins, apoptosis cultured human cells matched...
This study was conducted to evaluate the effects of graded concentrations (10(-8), 10(-7) and 10(-6) M) progesterone receptor (PR) modulator CDB-2914 on protein contents PR, vascular endothelial growth factor (VEGF), adrenomedullin (ADM) their receptors in cultured human uterine leiomyoma matching myometrial cells.PR-A, PR-B, VEGF-A, VEGF-B, VEGF (VEGFR)-1, VEGFR-2, ADM (ADMR) were assessed by Western blot analysis.Treatment with 100 ng/ml increased VEGF-B cells normal cells. The concomitant...
Calmodulin (CaM) binding sites were recently identified on the cytoplasmic loop (CL) of at least three α-subfamily connexins (Cx43, Cx44, Cx50), while Cx40 does not have this putative CaM domain. The purpose study was to examine functional relevance Cx43 site Ca(2+)-dependent regulation gap junction proteins formed by and Cx40. Dual whole cell patch-clamp experiments performed stable murine Neuro-2a cells expressing or Addition ionomycin increase external Ca(2+) influx reduced conductance...
Concurrent chemoradiotherapy (CCRT) involves the simultaneous administration of chemotherapy and radiotherapy, in which low-dose enhances effectiveness radiotherapy. This combined approach mitigates tumor recurrence metastasis, ultimately improving patient prognosis. The primary mechanism behind increased radiosensitivity induced by concurrent inhibiting cell repair complementary effects radiotherapy on different phases cycle. Despite CCRT application patients with locally advanced cervical...
Background: A growing number of patients with early-stage cervical cancer opt for local tumor resection to preserve the uterus. Nevertheless, there is still a dearth long-term comparisons between destruction/excision (LTD/LTE) and total hysterectomy(TH), optimal target population excision remains uncertain. Methods: multicenter retrospective study utilizing Surveillance, Epidemiology, End Results (SEER) database compare outcomes LTD/LTE TH in stage IA was conducted. 1:1 propensity score...
This study developed and evaluated an automatic segmentation model based on the Mamba framework (AM-UNet) for rapid precise delineation of high-risk clinical target volume (HRCTV) organs at risk (OARs) in cervical cancer brachytherapy. Using 694 CT scans from 179 patients, performance five models (AM-UNet, UNet, DeepLab V3, UNETR nnU-Net) was compared. The were assessed using Dice similarity coefficient (DSC), 95% Hausdorff distance (HD95), dose-volume index (DVI). AM-UNet achieved mean DSCs...
The present study was conducted to evaluate the effects of progesterone receptor modulator CDB-2914 on proliferative activity and apoptosis in cultured human uterine leiomyoma cells. Isolated cells were subcultured phenol red-free DMEM supplemented with 10% fetal bovine serum for 120 h then stepped down serum-free conditions 12, 24, 48, 96 absence or presence graded concentrations (10−9, 10−8, 10−7, 10−6m). number viable determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazodium...
Effects of progesterone receptor modulator CDB-2914 on the expression extracellular matrix (ECM) components were examined in cultured human uterine leiomyoma and myometrial cells. ECM metalloproteinase inducer (EMMPRIN), metalloproteinases (MMPs), tissue inhibitors MMP (TIMPs) collagen levels assessed by Western blot analysis, activity assay real-time RT–PCR. RNA interference (RNAi) EMMPRIN was performed using small interfering mRNA. In cells, treatment at concentrations greater than or...
Cx50 (connexin50), a member of the α-family gap junction proteins expressed in lens eye, has been shown to be essential for normal development. In present study, we identified CaMBD [CaM (calmodulin)-binding domain] (residues 141–166) intracellular loop Cx50. Elevations Ca2+ concentration effected 95% decline gj (junctional conductance) N2a cells that is likely mediated by CaM, because inclusion CaM inhibitor calmidazolium prevented this Ca2+-dependent decrease gj. The direct involvement...
A recent clinical trial demonstrated that selective progesterone receptor modulator asoprisnil is effective in reducing uterine leiomyoma volume. We investigated the effects of vitro on expression extracellular matrix (ECM)-remodeling enzymes and collagens cultured matching normal myometrial cells. The metalloproteinase inducer (EMMPRIN), metalloproteinases (MMPs), tissue inhibitors MMP (TIMPs) were assessed by western blot analysis. Untreated cells had significantly lower EMMPRIN (P <...
Abstract Purpose: This study (ClinicalTrials.gov identifier, NCT03676959) is an open, phase I dose-escalation and expansion investigating the safety efficacy of recombinant, fully human anti–programmed death ligand 1 (PD-L1) mAb socazolimab in patients diagnosed with recurrent or metastatic cervical cancer. Patients Methods: received every 2 weeks until disease progression. The was divided into a dose-expansion phase. Safety tolerability were primary endpoints objective response rate (ORR) 5...
Abstract Context: We previously demonstrated that asoprisnil, a selective progesterone receptor modulator, induces apoptosis of cultured uterine leiomyoma cells. This study was conducted to evaluate whether asoprisnil activates TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptotic pathway in and matching myometrial Objective Methods: After subculture phenol red-free DMEM supplemented with 10% fetal bovine serum for 120 h, cells were stepped down serum-free conditions 24 h the...
Metalloproteinase activities of a disintegrin and metalloproteinase 17 (ADAM17), amphiregulin (AREG), extracellular matrix inducer (EMMPRIN), metalloproteinases (MMPs) are involved in tumor biology. In patients with uterine cervical carcinoma, the expression prognostic significance ADAM17 remain to be fully elucidated. The ADAM17, AREG, EMMPRIN, phospho-epidermal growth factor receptor (p-EGFR), phospho-extracellular signal-regulated kinase (p-ERK), MMP-2, MMP-9 was assessed by...
Objective To assess the utility of predictive models using ultrasound radiomic features to predict cervical lymph node metastasis (CLNM) in solitary papillary thyroid carcinoma (PTC) patients. Methods A total 570 PTC patients were included (456 training set and 114 testing set). Pyradiomics was employed extract from preoperative images. After dimensionality reduction meticulous selection, we developed radiomics various machine learning algorithms. Univariate multivariate logistic regressions...
Histone deacetylase inhibitors (HDACIs) are being investigated as novel therapies for cancer, inflammation, neurodegeneration, and heart failure. The effects of HDACIs on the functional expression cardiac gap junctions (GJs) essentially unknown. purpose this study was to determine trichostatin A (TSA) vorinostat (VOR) GJ in ventricular cardiomyocytes. HDAC inhibition connexin43 (Cx43) assembly were examined primary cultured neonatal mouse myocytes. TSA VOR reduced Cx43 mRNA, protein...