A5002203423- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Crop Yield and Soil Fertility
- RNA Research and Splicing
- Viral Infections and Outbreaks Research
- Wastewater Treatment and Nitrogen Removal
- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Bioinformatics and Genomic Networks
- Kruppel-like factors research
- Metalloenzymes and iron-sulfur proteins
- Gene Regulatory Network Analysis
Corteva (United States)
2019
Virginia Tech
2007-2010
Molecular Sciences Software Institute
2008
Biotechnology Institute
2006
University of Maryland, Baltimore
2006
Sepragen Corporation (United States)
1998-2005
Pioneer Hi-Bred
2003
Cornell University
1986-1993
Dow Chemical (United States)
1991
Columbia University
1986
The International Strawberry Sequencing Consortium reports the draft genome of woodland strawberry (Fragaria vesca). this diploid species should serve as a reference for Fragaria genus, cultivated × ananassa) is an octoploid where F. vesca predicted to be subgenome donor. strawberry, (2n = 2x 14), versatile experimental plant system. This diminutive herbaceous perennial has small (240 Mb), amenable genetic transformation and shares substantial sequence identity with other economically...
A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided cost-effective assembly the genome sequence domestic turkey (Meleagris gallopavo). Heterozygosity sequenced source allowed discovery more than 600,000 high quality single nucleotide variants. Despite this heterozygosity, current (∼1.1 Gb) includes 917 Mb assigned to specific chromosomes. Annotation identified nearly 16,000 genes, 15,093 recognized as protein...
Abstract Oxalate oxidase (OXO) converts oxalic acid (OA) and O2 to CO2 hydrogen peroxide (H2O2), acts as a source of H2O2 in certain plant-pathogen interactions. To determine if the produced by OXO can function messenger for activation defense genes confer resistance against an OA-producing pathogen, we analyzed transgenic sunflower (Helianthus annuus cv SMF3) plants constitutively expressing wheat (Triticum aestivum) gene. The leaf tissues could degrade exogenous OA generate H2O2....
To determine the scope of gene expression controlled by maize transcription factors C1/R and P, which are responsible for activating flavonoid synthesis, we used GeneCalling, an open-ended, gel-based, mRNA-profiling technology, to analyze cell suspension lines inbred Black Mexican Sweet (BMS) that harbored estradiol-inducible versions these factors. BMS cells were transformed with a continually expressed estrogen receptor/maize C1 activator domain fusion (ER–C1) either R (CRC), or luciferase...
Summary The disease resistance gene Pto encodes a serine/threonine protein kinase that confers in tomato to Pseudomonas syringae pv. strains express the effector AvrPto. Pto‐mediated bacterial speck also requires Prf, with leucine‐rich repeats and putative nucleotide‐binding site, although role of Prf defense pathway is not known. We used GeneCalling, an open‐architecture, mRNA‐profiling technology, identify genes are either induced or suppressed leaves 4 h after infection Pto‐ Prf‐mediated...
The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as vaccine in vaccination of cattle against brucellosis for six decades. Despite many studies, the physiological and molecular mechanisms causing attenuation are not known. We have applied pyrosequencing technology together with conventional sequencing to rapidly comprehensively determine complete genome sequence S19. main goal this study is identify candidate virulence genes by systematic comparative analysis...
BackgroundThe design and construction of novel biological systems by combining basic building blocks represents a dominant paradigm in synthetic biology. Creating maintaining database these is way to streamline the fabrication complex constructs. The Registry Standard Biological Parts (Registry) most advanced implementation this idea.Methods/Principal FindingsBy analyzing inclusion relationships between sequences entries, we build network that can be related abstraction hierarchy....
Abstract Background Transcriptome sequencing using next-generation platforms will soon be competing with DNA microarray technologies for global gene expression analysis. As a preliminary evaluation of these promising technologies, we performed deep cDNA synthesized from the Microarray Quality Control (MAQC) reference RNA samples Roche's 454 Genome Sequencer FLX. Results We generated more that 3.6 million sequence reads average length 250 bp MAQC A and B introduced data analysis pipeline...
Emerging genome editing technologies hold great promise for the improvement of agricultural crops. Several related methods currently in development utilize engineered, sequence-specific endonucleases to generate DNA double strand breaks (DSBs) at user-specified genomic loci. These DSBs subsequently result small insertions/deletions (indels), base substitutions or incorporation exogenous donor sequences target site, depending on application. Targeted mutagenesis soybean (Glycine max) via...
At 443 kb, the map of Petunia hybrida line 3704 mitochondrial DNA is largest yet produced from a dicot plant. Regions similarity to known plant genes and chloroplast genome have been placed on master circle. One long repeated sequence, apparently active in recombination, present three copies. Two copies 6.6 kb occur direct orientation are separated by 199 kb. A third truncated copy 3.5 inverted relative other two others 99 145 The presence recombination repeats predicts multipartite...
Acute arenavirus disease in primates, like Lassa hemorrhagic fever humans, begins with flu-like symptoms and leads to death approximately 2 weeks after infection. Our goal was identify molecular changes blood that are related progression. Rhesus macaques (Macaca mulatta) infected intravenously a lethal dose of lymphocytic choriomeningitis virus (LCMV) provide model for infection humans. Blood samples taken before during the course were used monitor gene expression paralleled onset. Changes...
The centromere is a repeat-rich structure essential for chromosome segregation; with the long-term aim of understanding and function, we set out to identify cotton sequences. To isolate centromere-associated sequences from cotton, (Gossypium hirsutum) surveyed tandem dispersed repetitive DNA in genus. Centromere-associated elements other plants include repeats and, some cases, centromere-specific retroelements. Examination genomic survey yielded that did not localize centromere. However,...
A total of 96 independent Tn5 insertions within a 39-kilobase-pair (kbp) segment chromosomal DNA containing the three structural genes for nitrogenase (nifH, nifD, and nifK) from Bradyhizobium japonicum I110 were obtained in Escherichia coli transferred to wild-type strain by marker exchange. Individual transconjugants insertion inoculated onto Glycine max cv. Wilkin (soybeans) analyzed their effect on symbiotic nitrogen fixation. In addition genes, essential fixation (fix genes) located...
To assist breeding for increased resistance to root lodging in maize, an attempt was made identify genes that are associated with by profiling mRNA expression from two inbreds contrasting root-related traits. These were derived a common F(2) pool, selfed several generations and showed 75% relatedness based on 106 genetic markers. Under field conditions, the exhibited significant differences morphology lodging. Whole tissue collected at developmental stages inbred 100 101 grown 2 years. RNA...
A mitochondrial DNA (mtDNA) region termed the S-pcf locus has previously been correlated with cytoplasmic male sterility (CMS) in Petunia. In order to understand relationship of homologous sequences found elsewhere mtDNAs both CMS and fertile lines, structure genome Petunia line 3688 was determined by cosmid walking. The locus, which includes only copies genes for NADH dehydrogenase subunit 3 (nad3) small ribosomal protein 12 (rps12) be located on a circular map 396 kb, while second almost...
The aim was to evaluate the radioprotective properties of recombinant human fibroblast growth factor 20 (FGF-20; CG53135-05) in vitro and vivo examine its effects on known cellular pathways radioprotection. Relative transcript levels cyclooxygenase 2 (COX2), Mn-super oxide dismutase (SOD), CuZn-SOD, extracellular (EC)-SOD, nuclear respiratory (Nrf2), glutathione peroxidase 1 (GPX1) intestinal trefoil 3 (ITF3) genes, which are involved radiation response pathways, were assessed by reverse...
Targeted integration of recombinant DNA fragments into plant genomes by double-strand break (DSB) repair mechanisms has become a powerful tool for precision engineering crops. However, many targeting platforms require the screening transgenic events to identify low number targeted among more random insertion events. We developed an engineered transgene platform (ETIP) that uses incomplete marker genes at site enable rapid phenotypic and recovery upon functional reconstitution genes. The two...
The maize rhm1 mutant resists Bipolaris maydis, the causal agent of Southern corn leaf blight, by producing small necrotic lesions surrounded chlorotic haloes. and wild-type contain viable fungus in equal frequency, but fungal sporulation was markedly inhibited on rhm1. levels pathogenesis-related (PR) proteins chitinase, PR1, peroxidase differ little between wild type, with or without B. maydis inoculation. global mRNA profiles surveyed revealed hundreds cDNA fragments that were twofold...
Ribozymes are RNAs that can be designed to catalyze the specific cleavage or ligation of target RNAs. We have explored possibility using ribozymes in maize downregulate expression stearoyl–acyl carrier protein (Δ9) desaturase gene. Based on site accessibility and catalytic activity, several ribozyme constructs were transformed into regenerable lines. One these constructs, a multimer hammerhead linked selectable marker gene, was shown increase leaf stearate two 13 There concomitant decreases...
Activation Tagging, distributing transcriptional enhancers throughout the genome to induce transcription of nearby genes, is a powerful tool for discovering function genes in plants. We have developed transposable element system distribute novel activation tagging maize. The transposon built from Enhancer/Suppressor (En/Spm) and uses an engineered seed color marker show when excises. Both somatic germinal excision events can be detected by color. Spm-derived non-autonomous contains four...