A5004751787- Stress Responses and Cortisol
- Immune Response and Inflammation
- Tryptophan and brain disorders
- Antimicrobial Resistance in Staphylococcus
- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Yersinia bacterium, plague, ectoparasites research
- Metabolomics and Mass Spectrometry Studies
- Posttraumatic Stress Disorder Research
- Cancer, Lipids, and Metabolism
- Birth, Development, and Health
- Spaceflight effects on biology
- Molecular Biology Techniques and Applications
- Pharmacological Effects of Natural Compounds
- Bioinformatics and Genomic Networks
- Inflammatory mediators and NSAID effects
- Health, Environment, Cognitive Aging
- Bacillus and Francisella bacterial research
- Glycosylation and Glycoproteins Research
- Identity, Memory, and Therapy
- Fatty Acid Research and Health
- Adipose Tissue and Metabolism
- Cell Image Analysis Techniques
- Burn Injury Management and Outcomes
- Gene expression and cancer classification
Walter Reed Army Institute of Research
2010-2025
United States Army Medical Research and Development Command
2012-2024
United States Naval Medical Research Unit SOUTH
2023
59th Medical Wing
2023
University of California, San Francisco
2023
New York University
2023
United States Department of Veterans Affairs
2023
United States Army
2012-2022
Center for Environmental Health
2012-2022
Georgetown University
1971-2021
Human immunodeficiency virus (HIV) infection of brain macrophages and astroglial proliferation are central features HIV-induced nervous system (CNS) disorders. These observations suggest that glial cellular interactions participate in disease. In an experimental to examine this process, we found cocultures HIV-infected monocytes astroglia release high levels cytokines arachidonate metabolites leading neuronotoxicity. HIV-1ADA-infected cocultured with human glia (astrocytoma, neuroglia,...
The pathogenesis of central nervous system disease during human immunodeficiency virus type 1 (HIV-1) infection revolves around productive viral brain macrophages and microglia. Neuronal losses in the cortex subcortical gray matter accompany macrophage infection. question how ultimately leads to (CNS) pathology remains unanswered. Our previous work demonstrated high-level production tumor necrosis factor alpha, interleukin beta, arachidonic acid metabolites, platelet-activating (PAF) from...
Signal transduction pathways shared by different autocrine growth factors may provide an efficient approach to accomplish clinically significant control of lung cancer growth. In this study, we demonstrate that two activate 5-lipoxygenase action the arachidonic acid metabolic pathway in cell lines. Both increased production 5(S)-hydrooxyeicosa-6E,8Z,11Z,14Z-tetraeno ic (5-HETE), a major early product. Exogenously added 5-HETE stimulated vitro. Inhibition metabolism selective antagonists...
Objective: The purpose of this study was to assess the changes in gene expression near-infrared light therapy a model impaired wound healing. Background Data: Light-Emitting Diodes (LED), originally developed for NASA plant growth experiments space, show promise delivering deep into tissues body promote healing and human tissue growth. In paper we present effects LED treatment on wounds genetically diabetic mouse model. Materials Methods: Polyvinyl acetal (PVA) sponges were subcutaneously...
Many arachidonic acid metabolites function in growth signaling for epithelial cells, and we previously reported the expression of major enzymes human breast cancer cell lines. To evaluate role 5-lipoxygenase (5-LO) pathway on regulation, exposed cells to insulinlike factor-1 or transferrin, which increased levels 5-LO metabolite, 5(S)-hydrooxyeicosa-6E,8C,11Z,14Z-tetraenoic (5-HETE), by radioimmunoassay high-performance liquid chromatography. Addition 5-HETE resulted stimulation, whereas...
The purpose of this study was to identify enterotoxin genes from isolates coagulase-negative staphylococci and coagulase-positive obtained dairy products, responsible for 16 outbreaks food poisoning.From the pool 152 staphylococcal isolates, 15 representatives were selected study. tested presence coa femA genes, which are known be characteristic Staphylococcus aureus. After testing by polymerase chain reaction (PCR), 30 toxin immunoassay.Seven amplified gene subsequently reclassified as...
Abstract Active-duty Army personnel can be exposed to traumatic warzone events and are at increased risk for developing post-traumatic stress disorder (PTSD) compared with the general population. PTSD is associated high individual societal costs, but identification of predictive markers determine deployment readiness mitigation strategies not well understood. This prospective longitudinal naturalistic cohort study—the Fort Campbell Cohort study—examined value using a large multidimensional...
Post-traumatic stress disorder (PTSD) impacts many veterans and active duty soldiers, but diagnosis can be problematic due to biases in self-disclosure of symptoms, stigma within military populations, limitations identifying those at risk. Prior studies suggest that PTSD may a systemic illness, affecting not just the brain, entire body. Therefore, disease signals likely span multiple biological domains, including genes, proteins, cells, tissues, organism-level physiological changes....
DNA methylation patterns at specific cytosine-phosphate-guanine (CpG) sites predictably change with age and can be used to derive "epigenetic age", an indicator of biological age, as opposed merely chronological age. A relatively new estimator, called "DNAm GrimAge", is notable for its superior predictive ability in older populations regarding numerous age-related metrics like time-to-death, time-to-coronary heart disease, time-to-cancer. PTSD associated premature mortality frequently has...
Major depressive disorder (MDD) is associated with premature mortality and an independent risk factor for a broad range of diseases, especially those aging, such as cardiovascular disease, diabetes, Alzheimer's disease. However, the pathophysiology underlying increased rates somatic disease in MDD remains unknown. It has been proposed that represents state accelerated cellular several measures aging have developed recent years. Among metrics, estimators biological age based on predictable...
In this review, we compiled convolutional neural network (CNN) methods which have the potential to automate manual, costly and error-prone processing of medical images. We attempted provide a thorough survey improved architectures, popular frameworks, activation functions, ensemble techniques, hyperparameter optimizations, performance metrics, relevant datasets data preprocessing strategies that can be used design robust CNN models. also machine learning algorithms for statistical modeling...
Abstract HIV-1-infected brain macrophages participate in neurologic dysfunction through their continual secretion of neurotoxins. We previously demonstrated that astroglial cells activate monocytes to produce such neurotoxic activities. In this study, the mechanism underlying these monocyte secretory activities was unraveled and found dependent on HIV-1's ability prime for activation. LPS stimulation resulted an overexpression eicosanoids, platelet-activating factor (PAF), TNF-alpha. This...
A method has been developed for the rapid large scale isolation of plasma membranes and intact nuclei from RAJI lymphoid cells utilizing hypotonic lysis after intracellular loading with glycerol followed by combined flotation-sedimentation within a discontinuous sucrose gradient. Nuclei may be isolated in about 1 h 6 to 20 g cells. Intact nuclei, obtained 90 95% yield based on lysed cells, was differential centrifugation contained 16% DNA 30% total cell sialic acid. crude membrane fraction...
Abstract The two heterosaccharide chains of human transferrin have been isolated as glycopeptides, one which contained asparagine its sole amino acid residue while the other asparagine, glycine, serine, and, occasionally, threonine. Periodic oxidation either glycopeptide resulted in uptake 10 moles periodate and production 4 formate from sialic acid-containing 6 2 moles, respectively, acid-free glycopeptides. Formaldehyde (2 moles) was obtained only Periodate complete destruction galactose,...
Genetic factors appear to be highly relevant predicting differential risk for the development of post‐traumatic stress disorder (PTSD). In a discovery sample, we conducted genome‐wide association study (GWAS) PTSD using small military cohort (Systems Biology Biomarkers Consortium; SBPBC, N = 147) that was designed as case‐controlled sample exposed, recently returning veterans with and without combat‐related PTSD. A significant single nucleotide polymorphism (SNP), rs717947, at chromosome...
Posttraumatic stress disorder (PTSD) is associated with impaired major domains of psychology and behavior. Individuals PTSD also have increased co-morbidity several serious medical conditions, including autoimmune diseases, cardiovascular disease, diabetes, raising the possibility that systemic pathology might be identified by metabolomic analysis blood. We sought to identify metabolites are altered in male combat veterans PTSD. In this case-control study, we compared profiles from...