A5025467407- Urological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- Urinary and Genital Oncology Studies
- Congenital Anomalies and Fetal Surgery
- Epigenetics and DNA Methylation
- Infant Nutrition and Health
- Pediatric Urology and Nephrology Studies
- Bladder and Urothelial Cancer Treatments
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Hernia repair and management
- Digestive system and related health
- Urologic and reproductive health conditions
- Reproductive Biology and Fertility
- Bone and Dental Protein Studies
Children's Hospital of Chongqing Medical University
2022-2025
Chongqing Medical University
2022-2025
China International Science and Technology Cooperation
2022-2025
Mafb plays a significant role in the development and differentiation of various organs, tissues cells. Nevertheless, its control external genital cell proliferation function mechanism hypospadias remains unknown. In this study, expression foreskin fibroblasts was inhibited by siRNA. The Cell Count Kit-8 (CCK-8) assay showed increased after transfection, number cells entered S phase significantly via flow cytometry. Both mRNA protein levels cyclin E, cyclin-dependent kinase 2 (CDK2)...
MAFB is essential for regulating male-type urethral differentiation, and especially, its variation can contribute to hypospadias in mice. However, the potential mechanism still unclear. Here we observed that basic leucine zipper (bZIP) transcription factor CCAAT/enhancer-binding protein alpha (CEBPA) could promote human urothelium SV-HUC-1 growth. Moreover, CEBPA expression were reduced prepuce tissues of patients. Based on transcriptome sequencing analysis Western blot, knockdown was found...
Abstract Hypospadias is a common congenital abnormality of the penile. Abnormal spatiotemporal regulation critical genes interfering with urethral development leads to hypospadias. This study investigated role Rab25 in The expression levels various molecules foreskin tissues and fibroblasts were confirmed using PCR, immunohistochemistry, western blotting. Foreskin silencing constructed by lentivirus transfection. Using CRISPR/Cas9 technology, −/− mice generated. biological functions Cell...