A5077948097- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Animal Nutrition and Health
- Hormonal Regulation and Hypertension
- Genetic and phenotypic traits in livestock
- RNA Interference and Gene Delivery
- Aldose Reductase and Taurine
- Photosynthetic Processes and Mechanisms
- Adipose Tissue and Metabolism
- Agriculture and Biological Studies
- DNA and Nucleic Acid Chemistry
- ATP Synthase and ATPases Research
- RNA Research and Splicing
- Exercise and Physiological Responses
- Reproductive Physiology in Livestock
- Phytochemistry and Bioactive Compounds
- Animal Nutrition and Physiology
- Birth, Development, and Health
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Spectroscopy and Quantum Chemical Studies
- Reproductive Biology and Fertility
- Ruminant Nutrition and Digestive Physiology
- Bee Products Chemical Analysis
- Electrolyte and hormonal disorders
Novosibirsk State Agricultural University
2019-2025
Lomonosov Moscow State University
2015-2024
Yandex (Russia)
2024
Moscow State University
2015-2024
China University of Petroleum, East China
2022
Moscow Institute of Physics and Technology
2022
A N Bach Institute of Biochemistry
2022
Joint Institute for Nuclear Research
2022
Technische Universität Berlin
2022
Yantai Institute of Coastal Zone Research
2022
The article presents information regarding the metabolism of carbohydrates and minerals in Romanov sheep Kemerovo region. correlations among macro elements carbohydrate indices heavy metal levels organs tissues were ascertained. We determined average content, variability reference intervals glucose, amylase, lactate dehydrogenase blood serum. investigated relationship between these tissues. In breeding zone, content was analyzed soil, water, feed did not exceed MAC. determination macroand...
Ion pump, Na,K‐ATPase specifically binds cardiotonic steroids (CTS), which leads to inhibition of the enzyme activity and activation signaling network in cell. We have studied interaction with CTS two different types – marinobufagenin ouabain. shown that both inhibit same K i values, but binding ouabain is sensitive conformation while not. Furthermore, results structural changes Na,K‐ATPase. Our data allow explain diversity effects on receptor function caused by CTS.
Abstract Recent studies demonstrated that in addition to Na + ,K -ATPase inhibition cardiotonic steroids (CTSs) affect diverse intracellular signaling pathways. This study examines the relative impact of [Na ] i /[K -mediated and -independent transcriptomic changes triggered by endogenous CTSs ouabain marinobufagenin (MBG) human umbilical vein endothelial cells (HUVEC). We noted prolongation incubation increased apparent affinity for estimated loss [K gain . Six hour exposure HUVEC 100 3,000...
The structural organization of natural pigment-protein complexes provides a specific environment for the chromophore groups. Yet, proteins are inherently dynamic and conformationally mobile. In this work, we demonstrate heterogeneity chromophores C-phycocyanin (C-PC) from Arthrospira platensis. Part population trimeric C-PC is subject to spontaneous disturbances protein–protein interactions resulting in increased conformational mobility chromophores. Upon fluorescence excitation visible...
Active transport of sodium and potassium ions by Na,K-ATPase is accompanied the enzyme conformational transition between E1 E2 states. ATP ADP bind to in conformation with similar affinity but properties complexes these nucleotides are different. We have studied thermodynamics binding adenine at different temperatures using isothermal titration calorimetry. Our data indicate that β-phosphate involved complex formation increasing an order magnitude, while γ-phosphate does not affect it....
Sodium-potassium adenosine triphosphatase (Na,K-ATPase) creates a gradient of sodium and potassium ions necessary for the viability animal cells, it is extremely sensitive to intracellular redox status. Earlier we found that regulatory glutathionylation determines Na,K-ATPase sensitivity but role basal other modifications cysteine residues not clear. The purpose this study was detect oxidized, nitrosylated, or glutathionylated in Na,K-ATPase, evaluate possibility removing these assess their...
Prolonged hyperosmotic shrinkage evokes expression of osmoprotective genes via nuclear factor NFAT5-mediated pathway and activates Na+ influx hypertonicity-induced cation channels (HICC). In human umbilical vein endothelial cells (HUVEC) elevation intracellular sodium concentration ([Na+]i) triggers transcription dozens early response (ERG). This study examined the role monovalent cations in Na+i-sensitive ERGs iso- hyperosmotically shrunken HUVEC.Cell volume was measured by 3D...
Cardiotonic steroids (CTSs) are specific inhibitors of Na,K-ATPase (NKA). They induce diverse physiological effects and were investigated as potential drugs in heart diseases, hypertension, neuroinflammation, antiviral cancer therapy. Here, we compared the inhibition mode binding CTSs, such ouabain, digoxin marinobufagenin to NKA from pig rat kidneys, containing CTSs-sensitive (α1S) -resistant (α1R) α1-subunit, respectively. Marinobufagenin contrast ouabain interacted with α1S-NKA...
The authors presented the haematological blood parameters of Romanov breed sheep and their association with genotypes according to β-lactoglobulin locus. Blood plays a significant role in body as metabolism is carried out. It delivers nutrients oxygen cells organs removes metabolic waste products carbon dioxide. intensity processes can be judged from data. Since enzymes, activity, levels, biochemical adaptation are encoded genes, believe that composition animals is, certain extent, related...
In the article, authors conducted a comprehensive study of interior, including biochemical, physiological, physicochemical, cytogenetic, and other indicators in sheep Romanov breed Western Siberia. For study, blood serum samples were taken from 10 rams-producers 30 lambs at four months. Conventional methods took samples. Analyses performed biochemistry laboratory Novosibirsk State Agrarian University using technique competitive enzyme immunoassay with reagent kit “SteroidIFAtestosterone” for...
High-salt consumption contributes to the development of hypertension and is considered an independent risk factor for vascular remodelling, cardiac hypertrophy stroke incidence. Alterations in NO production, inflammation endothelial cell stiffening are now as plausible mediators cardiovascular dysfunction. We studied early responses cells (HUVEC) caused by a moderate increase extracellular sodium concentration. Exposure HUVEC elevated within physiological range up 24 h accompanied changes...
The assessment of the gene pool and phenofund agricultural breeds is an issue in livestock breeding. accuracy molecular genetic methods much higher than phenotypic observation order to improve breeds. It necessary establish relationship between genotype desired trait for their successful application. Therefore, purpose our study was genotypes at β-Lg locus with some biochemical blood parameters Romanov sheep. amount albumin, urea uric acid sheep studied. On a population 23 breed, we studied...