A5019840028- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Circadian rhythm and melatonin
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neonatal and fetal brain pathology
- Cannabis and Cannabinoid Research
- Tryptophan and brain disorders
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- Thermal Regulation in Medicine
- Electrolyte and hormonal disorders
- Anesthesia and Neurotoxicity Research
- Regulation of Appetite and Obesity
- Gut microbiota and health
- Epilepsy research and treatment
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Infant Health and Development
- Neural dynamics and brain function
- Neurotransmitter Receptor Influence on Behavior
University of Calgary
2015-2024
Alberta Children's Hospital
2011-2024
Czech Academy of Sciences, Institute of Physiology
2024
Allen Institute for Brain Science
2005-2024
Alberta Children's Hospital Research Institute
2015-2024
University of Florida Health
2019
Ontario Brain Institute
2005-2016
Institute of Neuroimmunology of the Slovak Academy of Sciences
2013
Health Sciences Centre
1985-2012
Institute of Infection and Immunity
2010
The presence and function of CB 2 receptors in central nervous system (CNS) neurons are controversial. We report the expression receptor messenger RNA protein localization on brainstem neurons. These functional were activated by a agonist, 2-arachidonoylglycerol, elevated endogenous levels endocannabinoids, which also act at 1 receptors. represent an alternative site action endocannabinoids that opens possibility nonpsychotropic therapeutic interventions using enhanced endocannabinoid...
Peripheral inflammation leads to a number of centrally mediated physiological and behavioral changes. The underlying mechanisms the signaling pathways involved in these phenomena are not yet well understood. We hypothesized that peripheral increased neuronal excitability arising from CNS immune response. induced gut by intracolonic administration 2,4,6-trinitrobenzene sulfonic acid (TNBS) adult male rats. To examine brain vivo , we administered pentylenetetrazole (PTZ; GABAergic antagonist)...
There are critical postnatal periods during which even subtle interventions can have long-lasting effects on adult physiology. We asked whether an immune challenge early development alter neuronal excitability and seizure susceptibility in adults. Postnatal day 14 (P14) male Sprague Dawley rats were injected with the bacterial endotoxin lipopolysaccharide (LPS), control animals received sterile saline. Three weeks later, extracellular recordings from hippocampal slices revealed enhanced...
Peripheral inflammatory diseases are often associated with behavioral comorbidities including anxiety, depression, and cognitive dysfunction, but the mechanism for these is not well understood. Changes in neuronal synaptic functions neuroinflammation may underlie abnormalities. We have used a model of colonic inflammation induced by 2,4,6-trinitrobenzenesulfonic acid Sprague Dawley rats to identify inflammation-induced changes hippocampal transmission. Hippocampal slices obtained 4 d after...
Simultaneous microdialysis in blood and brain has been used to monitor the release of both oxytocin vasopressin into systemic circulation (jugular vein/right atrium) within hypothalamic supraoptic nucleus rats. Both home-made probes for revealed detectable nonapeptide concentrations under basal conditions differential responses a variety stimuli. In urethane-anesthetized male rats, bilateral stimulation by microdialyzing hypertonic medium (1 M NaCl) not only significantly increased...
Neuropeptide Y (NPY), the most abundant peptide in mammalian CNS, has been shown to inhibit excitatory neurotransmission presynaptically at stratum radiatum-CA1 synapse vitro rat hippocampal slice. We examined site and mechanism of this inhibition a series intra- extracellular recordings areas CA1 CA3, source much synaptic input neurons. NPY's inhibitory action was unaffected by high concentrations antagonists bicuculline, theophylline, or atropine, suggesting that it does not act...
Summary: Purpose: Despite the prevalence of febrile convulsions (FCs), their pathophysiology has remained elusive. We tested hypothesis that components immune response, particularly proinflammatory cytokine interleukin‐1β (IL‐1β) and its naturally occurring antagonist interleukin‐1 receptor (IL‐1ra) may play a role in genesis FC. Methods: Postnatal day 14 rats were treated with lipopolysaccharide (LPS; 200 μg/kg, i.p.) followed by subconvulsant dose kainic acid (1.75 mg/kg, i.p.). Brains...
We have examined potential functions of nitric oxide (NO) within the paraventricular nucleus (PVN) in urethan-anesthetized male Sprague-Dawley rats. Initial experiments demonstrated microinjection 50 pmol NO donor, sodium nitroprusside (SNP), directly into PVN resulted significant decreases mean blood pressure (BP) (-3,312 +/- 1,189 mmHg/s over 300-s response time; P < 0.05). To determine whether such effects were attributable to SNP-induced release, was administered by bilateral...
1. Neuropeptide tyrosine (neuropeptide Y, NPY), a recently isolated endogenous brain peptide, reduces the extracellular population spike evoked by stimulation of stratum radiatum in area CA1 vitro rat hippocampal slice, without reducing antidromically spike. To test hypothesis that NPY acts presynaptically, intracellular recordings were made pyramidal neurones vitro. 2. Bath application 10(‐6) M‐NPY causes long‐lasting (1‐1.5 h), reversible reduction orthodromically excitatory post‐synaptic...
In anesthetized male Sprague-Dawley rats, intraventricular injection of 25–5000 pmol AVP caused short-latency, dose-related increases in blood pressure. Blood pressure occurred before any changes urine flow were observed, suggesting that the was acting centrally. Similar observed after central into homozygous Brattleboro rats which lack AVP, indicating these effects not due to release pituitary stores. These results raise possibility endogenous plays a role control
The Islet Autoantibody Standardization Program (IASP) aims to improve the performance of immunoassays measuring type 1 diabetes (T1D)-associated autoantibodies and concordance results among laboratories. IASP organizes international interlaboratory assay comparison studies in which blinded serum samples are distributed participating laboratories, followed by centralized collection analysis results, providing participants with an unbiased comparative assessment. In this report, we describe...
Oxytocin is released within the supraoptic nucleus during parturition and suckling. During suckling, such release important in positive feedback stimulation of oxytocin neurons. We have investigated whether this hypothalamic (1) acts on local receptors to further amplify its own a manner (2) critically involved regulation delivery process. To examine effect antagonist nucleus, microdialysates were sampled before either vehicle or was infused adjacent microdialysis probe directly into after...