A5006520373- Asthma and respiratory diseases
- Mast cells and histamine
- Cell Adhesion Molecules Research
- Urticaria and Related Conditions
- Platelet Disorders and Treatments
- Inflammatory mediators and NSAID effects
- Pulmonary Hypertension Research and Treatments
- Neuropeptides and Animal Physiology
- Eicosanoids and Hypertension Pharmacology
- Allergic Rhinitis and Sensitization
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- Cancer, Stress, Anesthesia, and Immune Response
- COVID-19 Clinical Research Studies
- Receptor Mechanisms and Signaling
- Long-Term Effects of COVID-19
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Angiogenesis and VEGF in Cancer
- SARS-CoV-2 and COVID-19 Research
- Pharmacological Effects and Assays
- S100 Proteins and Annexins
- Wound Healing and Treatments
- Food Allergy and Anaphylaxis Research
- Nitric Oxide and Endothelin Effects
Vicore Pharma (Sweden)
2020-2024
Karolinska Institutet
1999-2023
École des Hautes Études en Santé Publique
2023
Inserm
2023
Université de Rennes
2023
Centre National de la Recherche Scientifique
2023
Lantmännen
2017
AstraZeneca (United States)
2003
AstraZeneca (Brazil)
2001
Södertälje Sjukhus
2001
Inflammatory recruitment of leukocytes into the cerebrospinal fluid (CSF) during bacterial meningitis has been shown to con- tribute significantly neurological damage commonly as- sociated with this serious disease.In study we tested whether or not inhibition leukocyte rolling, a precondition for firm adhesion vascular endothelium in vivo, may reduce CSF and associated inflam- matory changes rabbits experimental meningitis.As documented by intravital microscopy small venules rabbit mesentery...
Intravital microscopy and determination of in vivo histamine release revealed that the cyclooxygenase inhibitor indomethacin reduced antigen-induced vasodilation while enhancing plasma extravasation, leukocyte accumulation, cheek pouches immunized hamsters. Topical application prostaglandin E2 (PGE2, 30 nM) totally reversed indomethacin-induced potentiation inflammatory reaction to antigen challenge suppressed both leakage also absence indomethacin. On other hand, PGE2, which per se caused...
Leukocyte rolling and firm adhesion at the venular endothelium are two discrete events in cellular inflammatory response mediated via selectin integrin molecules, respectively. The dependency of chemoattractant‐induced leukocyte on preceding interaction was investigated rat mesenteric microvessels through use intravital microscopy. dose‐dependently inhibited by systemic treatment with sulphated polysaccharide fucoidin. following stimulation chemotactic peptide fMLP similarly when challenge...
Biological activities of Lipoxin A include lung strip contraction and dilation arterioles in vivo. Acta Physiol Scand 130 , 643–647. Received 7 December 1986, accepted 27 February 1987. ISSN 0001–6772. Departments Physiology Physiological Chemistry, Karolinska Institute, Department Experimental Medicine, Pharmacia AB, Sweden. ([5s,6r,15s]‐5,6,15–trihydroxy‐7,9,13–trans‐II‐cis‐eicosatetraenoic acid), a recently characterized lipoxygenation product arachidonic acid, submicromolar...
The main objective of this study was to analyse the role and mode action mast cell mediator histamine in leukocyte‐endothelium interactions small venules vivo . For purpose, we used a histological approach (combined with intravital microscopy) that allows studies rapid mediator‐induced venular leukocyte accumulation, reflecting rolling, undisturbed microcirculation rat mesentery where rolling is normally absent. We first examined relative importance 5‐hydroxytryptamine (5‐HT) acute...
Leukocyte rolling and adhesion are generally observed in venules but rarely arterioles. With the use of intravital microscopy, we found that a 4-h treatment with interleukin-1beta (IL-1beta) tumor necrosis factor-alpha (TNF-alpha) dose dependently induced leukocyte arterioles mouse cremaster muscle. The response lasted more than 24 h was completely inhibited by sulfated polysaccharide fucoidin. Moreover, costimulation IL-1beta TNF-alpha for 4 synergistically increased arteriolar rolling,...
Substantial evidence supports the involvement of renin-angiotensin system in pulmonary hypertension (PH), and angiotensin II type 2 receptor (AT
ABSTRACT The inflammatory response in bacterial meningitis is mediated by cytokines, such as tumor necrosis factor alpha (TNF-α) and interleukin-1 (IL-1), which are produced the subarachnoid space different cells, e.g., leukocytes, astrocytes, microglia. recruitment of leukocytes into cerebrospinal fluid (CSF) has been shown to contribute neurological damage this disease, a process could be enhanced treatment with antibiotics. In study, we have used rabbit model for two sets experiments...
Intravital microscopy of the hamster cheek pouch was used to examine influence vasodilator prostanoids (prostaglandin E 2 (PGE ), PGI forskolin, and nitroprusside on microvascular changes during acute inflammation induced by antigen or histamine. The results extend our previous finding that PGE modulates allergic histamine release in model. actions arachidonic acid different cyclo‐oxygenase products , PGD PGF 2α thromboxane A (TXA )‐analogue U‐44069) were first compared with respect their...
Anti‐inflammatory actions of heparin and related glycosaminoglycans have been described in the literature. Here, we used intravital microscopy rat mesentery microcirculation to examine effects locally administered on leukocyte rolling chemoattractant‐induced firm adhesion. It was found that topical application reduced N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP)‐induced Notably, inhibitory action not dose‐dependent, but rather a biphasic dose‐response found, i.e. low (2 20 iu ml −1 ) high...
Topical antigen challenge in cheek pouches of immunized hamsters led to an acute inflammatory reaction which was characterized by intravital microscopy. The response consisted short‐lasting arteriolar spasm, followed leakage plasma, vasodilation, and accumulation leucocytes. Several observations indicated that the due mast cell activation. Thus, a very similar seen after with compound 48/80, both 48/80 degranulated numerous cells present pouch. In addition, fluorescein‐labelled bound...
Although intravital microscopy is the method of choice for observation inflammatory leukocyte rolling and adhesion in small venules vivo , a problem with this technique that surgical exposure suitable tissues per se triggers mechanism. In study, we describe an approach to investigate induction undisturbed microvessels. For purpose, microscopic rat mesentery was combined histological determination intravascular concentrations polymorphonuclear mononuclear leukocytes (PMNL MNL). By relating...
1 Intravital microscopy of the rat mesentery was used to examine interactions between histamine and chemoattractant leukotriene B4 (LTB4) with regard leukocyte adhesion in postcapillary venules. 2 Topical administration caused a four fold potentiation LTB4-induced adhesion. 3 Histamine significantly increased rolling flux by 25%, this effect on strictly blood flow-dependent, i.e. we found significant positive correlations both flow total flux, while no changes fraction or velocity were...
BackgroundClinical research supports that exercise-induced bronchoconstriction (EIB) is caused by hyperosmolar triggering of mast cells. The reaction can be mimicked inhalation mannitol, but it has paradoxically previously not been possible to replicate this mode action mannitol in isolated airways.ObjectiveWe sought establish an ex vivo model EIB human small bronchi.MethodsSmall bronchi (inner diameter, 0.5-2 mm) from macroscopically healthy lung tissue were obtained 48 patients and mounted...
Our main aim was to investigate the effect of a single oral dose C21, selective angiotensin II type 2 receptor agonist, on cold-induced vasoconstriction in SSc-related RP.