- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Natural product bioactivities and synthesis
- Plant tissue culture and regeneration
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Radiation Effects and Dosimetry
- Postharvest Quality and Shelf Life Management
- Microbial Metabolic Engineering and Bioproduction
- Reproductive biology and impacts on aquatic species
- Environmental Toxicology and Ecotoxicology
- Plant Gene Expression Analysis
- Coleoptera: Cerambycidae studies
- Genetic Mapping and Diversity in Plants and Animals
- Phytochemistry and Biological Activities
- Plant Disease Management Techniques
- Leaf Properties and Growth Measurement
- Enzyme Production and Characterization
- Mitochondrial Function and Pathology
- Pharmacological Effects of Natural Compounds
- GABA and Rice Research
- Plant Genetic and Mutation Studies
- Botanical Studies and Applications
Nanjing Normal University
2025
Guangzhou University of Chinese Medicine
2021-2024
China Pharmaceutical University
2024
South China Agricultural University
2012
Wurfbainia villosa is a well-known medicinal and edible plant that widely cultivated in the Lingnan region of China. Its dried fruits (called Fructus Amomi) are broadly used traditional Chinese medicine for curing gastrointestinal diseases rich volatile terpenoids. Here, we report high-quality chromosome-level genome assembly W. with total size approximately 2.80 Gb, 42 588 protein-coding genes, very high percentage repetitive sequences (87.23%). Genome analysis showed likely experienced...
Bis(4-hydroxyphenyl) methane (BPF), as a bisphenolic compound, has toxic effects on organisms such endocrine disruption and immobilization of growth development. This study evaluated the effect concentrations BPF Chironomus tentans investigated impact exposure at various sub-lethal growth, development, reproductive capacity different instars C. tentans. The results demonstrated that 2.0, 2.5, 3.0, 4.0 mg·L−1 delayed pupation, inhibited development imaginal discs, caused an initial rise...
Bornyl acetate (BA) is known as a natural aromatic monoterpene ester with wide range of pharmacological and biological activities. Borneol acetyltransferase (BAT), catalyzing borneol acetyl-CoA to synthesize BA, alcohol acetyltransferase, which belongs the BAHD super acyltransferase family, however, BAT, responsible for biosynthesis has not yet been characterized. The seeds Wurfbainia villosa (homotypic synonym: Amomum villosum) are rich in BA. Here we identified 64 members gene family from...
Linarin (acacetin-7-O-rutinoside), isorhoifolin (apigenin-7-O-rutinoside), and diosmin (diosmetin-7-O-rutinoside) are chemically structurally similar flavone rutinoside (FR) compounds found in Chrysanthemum L. (Anthemideae, Asteraceae) plants. However, their biosynthetic pathways remain largely unknown. In this study, we cloned compared FRs genes encoding rhamnosyltransferases (RhaTs) among eight accessions of polyploids. We also biochemically characterized RhaTs plants Citrus (Citrus...
Plant mutants are important bio-resources for crop breeding and gene functional studies. Conventional methods generating mutant libraries by mutagenesis of seeds with physical or chemical agents low efficiency. Here, we developed a highly-efficient ethyl methanesulfonate (EMS) system based on suspension-cultured cells, rice (Oryza sativa L.) as an example. We show that treatment tiny cell clusters 0.4% EMS 18-22 h followed differentiation regeneration produced high 29.4% independent lines...
The fruits of Amomum villosum and longiligulare are both used medicinally as Fructus Amomi the famous traditional Chinese medicine, however, medicinal quality A. is better than that . Volatile terpenoids in seeds, especially bornyl acetate borneol, components Amomi. volatile transcriptome developing seeds were compared this study. result revealed borneol contents higher Additionally, six terpenoid synthase genes ( AlTPS1–AlTPS6 ) screened from , AlTPS2 AlTPS3 found to share 98 83% identity...
In plant secondary metabolite biosynthesis, acylation is a diverse physiological process, with BAHD acyltransferases playing an essential role. Borneol acetyltransferase (BAT) alcohol acetyltransferase, which catalyzes borneol and acetyl-CoA to synthesize bornyl acetate (BA). However, the enzymes involved in biosynthesis of BA have so far only been characterized Wurfbainia villosa, studies on WvBATs conducted vitro, catalytic activity was relatively low. this research, three genes (WlBAT1,...