A5068876679- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Genetic Syndromes and Imprinting
- Rice Cultivation and Yield Improvement
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Genetic and phenotypic traits in livestock
- Immune Response and Inflammation
- Inflammasome and immune disorders
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Genetic Associations and Epidemiology
- Chromosomal and Genetic Variations
- IL-33, ST2, and ILC Pathways
- Fetal and Pediatric Neurological Disorders
- Genomics and Rare Diseases
- Telomeres, Telomerase, and Senescence
Sichuan University
2023-2025
West China Hospital of Sichuan University
2023-2025
Second Hospital of Hebei Medical University
2025
Hebei Medical University
2025
Hunan Agricultural University
2020-2023
Nanjing Agricultural University
2023
University of Cambridge
2015-2021
Shanghai First Maternity and Infant Hospital
2014-2021
Cancer Research UK Cambridge Center
2015
Reproductive Medicine Associates of New Jersey
2007-2014
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods aneuploidy screening blastomeres same has not been conducted and may indicate whether overestimated due to technical inconsistency rather than biological phenomena. The present study investigates prevalence abnormality found with different single cell techniques. Thirteen arrested embryos were studied. Each was biopsied into individual cells (n = 160)....
Aneuploidy represents the most prevalent form of genetic instability found in human embryos and is leading cause miscarriage developmental delay newborns. Telomere DNA deficiency associated with genomic somatic cells may play a role development aneuploidy commonly female germ embryos. To test this hypothesis, we developed method capable quantifying telomere parallel 24-chromosome screening from same oocyte or embryo biopsy. Aneuploid polar bodies possessed significantly less than euploid...
There is renewed interest in using animal oocytes to reprogram human somatic cells. Here we compare the reprogramming of nuclei obtained from and sources. Comparative analysis gene expression morula-stage embryos was carried out single-embryo transcriptome amplification global analyses. Genomic DNA fingerprinting PCR confirmed that nuclear genome cloned originated donor cell. Although human-human, human-bovine, human-rabbit clones appeared morphologically similar continued development morula...
Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number commercially available WGA kits have been independently utilized previous single-cell microarray studies. However, direct comparison their performance on has not conducted. The present study demonstrates that among previously published methods, GenomePlex protocol provides the best...
Wurfbainia villosa is a well-known medicinal and edible plant that widely cultivated in the Lingnan region of China. Its dried fruits (called Fructus Amomi) are broadly used traditional Chinese medicine for curing gastrointestinal diseases rich volatile terpenoids. Here, we report high-quality chromosome-level genome assembly W. with total size approximately 2.80 Gb, 42 588 protein-coding genes, very high percentage repetitive sequences (87.23%). Genome analysis showed likely experienced...
The purpose of this study was to compare the clinical outcome testicular spermatozoon versus ejaculated in treatment infertile males with high sperm DNA damage, referred as fragmentation index (DFI), that attending intracytoplasmic injection (ICSI) programme terms pregnancy, births delivered primary and pregnancy loss embryo fertilisation secondary outcome. A total 102 fulfilling inclusion criteria were enrolled present study. Of males, 61 underwent combined ICSI while remaining 41 applied...
This study explores the causal relationships between five major lipids, 249 circulating metabolites, and four diabetic retinopathy (DR) outcomes: overall DR, background severe proliferative DR (PDR). We aim to identify plasma proteins that mediate these effects, offering insights into potential therapeutic targets. conducted metabolome-wide Mendelian randomization (MR) analyses assess associations outcomes. Multivariable MR (MVMR) proteome-wide mediated (two-step MR) were performed ensure...
<title>Abstract</title> <bold>Background:</bold> This study explores the causal relationships between five major lipids, 249 circulating metabolites, and four diabetic retinopathy (DR) outcomes: overall DR, background severe proliferative DR (PDR). We aim to identify plasma proteins that mediate these effects, offering insights into potential therapeutic targets. <bold>Methods:</bold> conducted metabolome-wide Mendelian randomization (MR) analyses assess associations outcomes. Multivariable...
Members of the Ashkenazi Jewish community are at an increased risk for inheritance numerous genetic diseases such that carrier screening is medically recommended. This paper describes development and evaluation 30 TaqMan allelic discrimination qPCR assays 29 mutations on 2 different high-throughput platforms. Four these in GBA gene successfully examined using short amplicons due to qualitative nature discrimination. Two systems were tested their reliability (call rate) consistency with...
Abstract Genetic improvement of grain quality is more challenging in hybrid rice than inbred due to additional nonadditive effects such as dominance. Here, we describe a pipeline developed for joint analysis phenotypes, effects, and generations (JPEG). As demonstration, analyze 12 traits 113 lines (male parents), five tester (female 565 (113×5) their hybrids. We sequence the parents single nucleotide polymorphisms calling infer genotypes Genome-wide association studies with JPEG identify 128...
Recently, in-vitro maturation (IVM) of immature human oocytes recovered from non-stimulated follicles has been applied in the treatment infertility. However, previous reports, very few embryos cultured conventional medium have reached expanded blastocyst stage following and fertilization (IVM/IVF). The objective this study was to investigate whether developmental competence IVM/IVF could be enhanced by use a ampullary cell co-culture system. Immature were aspirated small at Caesarean section...
Remifentanil with appropriate pharmacological properties seems to be an ideal alternative epidural analgesia during labour. A retrospective cohort study was undertaken assess the efficacy and safety of remifentanil intravenous patient-controlled (IVPCA) compared analgesia. Medical records 370 primiparas who received IVPCA or were reviewed. Pain sedation scores, overall satisfaction, extent pain control, maternal side effects neonatal outcome as primary observational indicators collected....