Prihardi Kahar

ORCID: 0000-0003-1006-2283
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About
Contact & Profiles
Research Areas
  • Biofuel production and bioconversion
  • Microbial Metabolic Engineering and Bioproduction
  • Enzyme Catalysis and Immobilization
  • Fungal and yeast genetics research
  • biodegradable polymer synthesis and properties
  • Catalysis for Biomass Conversion
  • Microplastics and Plastic Pollution
  • Biopolymer Synthesis and Applications
  • Enzyme Production and Characterization
  • Polyamine Metabolism and Applications
  • Viral Infectious Diseases and Gene Expression in Insects
  • Biochemical and biochemical processes
  • Lignin and Wood Chemistry
  • Microbial Natural Products and Biosynthesis
  • Advanced biosensing and bioanalysis techniques
  • Biodiesel Production and Applications
  • Carbohydrate Chemistry and Synthesis
  • CRISPR and Genetic Engineering
  • Oil Palm Production and Sustainability
  • Fermentation and Sensory Analysis
  • GABA and Rice Research
  • Cancer therapeutics and mechanisms
  • Advanced Nanomaterials in Catalysis
  • Plant nutrient uptake and metabolism
  • Carbon dioxide utilization in catalysis

Kobe University
2016-2025

Kobe Institute Of Computing
2023

Meisei University
2010-2013

Tokyo Institute of Technology
2003-2006

Gifu University
2001-2002

Shizuoka University
1997-2002

The potential of Streptomyces jeddahensis for lipid production was investigated, focusing on its ability to utilize various carbon sources under different temperature conditions. Glucose and fatty acid derivatives (sodium hexanoate, sodium octanoate, decanoate) were tested at 30 °C, 37 45 °C optimize growth biosynthesis. results revealed that the optimal accumulation, with octanoate leading highest content. While declined significant persisted, indicating an adaptive stress response. Gene...

10.3390/fermentation11020045 article EN cc-by Fermentation 2025-01-21

Integrating heterogeneous genes is widely used in metabolic engineering to produce D-lactic acid (D-LA), an essential compound bioplastics and pharmaceuticals. However, research on the effects of integrating various loci gene expression, especially regarding flocculation behavior, remains limited. This study constructed Saccharomyces cerevisiae strains by incorporating a codon-optimized D-LDH from Leuconostoc pseudomesenteroides (LpDLDH) into specific genomic CYB2, PDC1, MPC1, PDC6, ADH1,...

10.3390/microorganisms13030618 article EN cc-by Microorganisms 2025-03-07

Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores various plants. However, utilization of mannans sustainable resources has been less advanced biofuel development. Based on a yeast cell surface-display technology that enables immobilization multiple enzymes walls, we constructed recombinant Saccharomyces cerevisiae strain co-displays β-mannanase β-mannosidase; this is expected to facilitate ethanol fermentation using mannan biomass...

10.1186/s13068-016-0600-4 article EN cc-by Biotechnology for Biofuels 2016-09-02

Abstract Chinese hamster ovary (CHO) cells are the primary host used for biopharmaceutical protein production. The engineering of CHO to produce higher amounts biopharmaceuticals has been highly dependent on empirical approaches, but recent high-throughput “omics” methods changing situation in a rational manner. Omics data analyses using gene expression or metabolite profiling make it possible identify key genes and metabolites antibody Systematic omics approaches different types time-series...

10.1038/srep43518 article EN cc-by Scientific Reports 2017-03-02

Mannan endo-1,4-β-mannosidase (commonly known as β-mannanase) catalyzes a random cleavage of the β-D-1,4-mannopyranosyl linkage in mannan polymers. The enzyme has been utilized biofuel production from lignocellulose biomass, well mannooligosaccharides (MOS) for applications feed and food industries. We aimed to obtain β-mannanase, such polymer utilization, actinomycetes strains isolated Indonesia. Strains exhibiting high mannanase activity were screened, one strain belonging genus...

10.1186/s13568-017-0401-6 article EN cc-by AMB Express 2017-05-19

Pretreatment of lignocellulosic biomass is crucial yet challenging for sustainable energy production. This study focuses on enhancing enzymatic accessibility cellulose in oil palm empty fruit bunches by optimizing pretreatment parameters to improve glucose and ethanol yields while reducing fermentation inhibitors. It evaluates the impact maleic acid concentrations biorefinery processes. High (>25% w/w) may allow reuse offer benefits over lower concentrations, such as enhanced delignification...

10.1021/acsenvironau.4c00045 article EN cc-by-nc-nd ACS Environmental Au 2024-11-21

Meyerozyma guillermondii is an interesting oleaginous yeast with considerable potential for biotechnological applications. This demonstrates the ability to utilize palm acid oil (PAO), a low-cost and renewable feedstock, as carbon source, making it sustainable candidate single-cell production. Under optimal conditions 4% of PAO, M. can accumulate lipids approximately 45% its dry cell weight (DCW). Notably, expression level PEX14, gene associated peroxisomal biogenesis, increases higher PAO...

10.20944/preprints202501.0122.v1 preprint EN 2025-01-03
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