A5085057723- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- T-cell and B-cell Immunology
- Ocular Diseases and Behçet’s Syndrome
- Enzyme Catalysis and Immobilization
- Chemical Synthesis and Analysis
- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- Phytochemical compounds biological activities
- Curcumin's Biomedical Applications
- Marine Sponges and Natural Products
- Enzyme Structure and Function
- Synthetic Organic Chemistry Methods
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Autoimmune and Inflammatory Disorders Research
- Biochemical and biochemical processes
- Plant-Microbe Interactions and Immunity
- Cyclopropane Reaction Mechanisms
- Biochemical and Structural Characterization
- Synthesis and Catalytic Reactions
- Reproductive System and Pregnancy
- Plant Gene Expression Analysis
The University of Tokyo
2016-2025
The Japanese Society of Gastroenterological Surgery
2020
Showa University
2020
Showa University Koto Toyosu Hospital
2020
Jikei University School of Medicine
2020
Iwate Medical University
2020
Bunkyo University
2018
RELX Group (Netherlands)
2018
Saarland University
2011-2017
Pharmaceutical Biotechnology (Czechia)
2017
Abstract Specialized or secondary metabolites are small molecules of biological origin, often showing potent activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis these natural products is governed by sets co-regulated physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs a standardized machine-readable way, Minimum Information Biosynthetic Gene cluster (MIBiG) data standard repository was initiated...
Abstract Current human leukocyte antigen (HLA) DNA typing methods such as the sequence‐based ( SBT ) and sequence‐specific oligonucleotide SSO generally yield ambiguous results because of probe design limitations or phase ambiguity for HLA allele assignment. Here we describe development application super high‐resolution single‐molecule SS‐SBT loci at 8‐digit level using next generation sequencing NGS ). which can determine an sequence derived from a single molecule is expected to solve...
<h3>Background</h3> Behçet disease (BD) is a rare, chronic, systemic, inflammatory disorder characterised by recurrent ocular, genital and skin lesions. Although its aetiology still uncertain, an intricate interplay between the environment (eg, viruses) host seems to initiate and/or perpetuate disease, although mechanism remains speculative. Since identification of <i>HLA-B*5101</i> (and more recently <i>MICA</i>) as susceptibility locus for BD, additional genetic locus/loci, whether inside,...
Benzastatins have unique structures probably derived from geranylated p-aminobenzoic acids. The indoline and tetrahydroquinoline scaffolds are presumably formed by cyclization of the geranyl moiety, but mechanism was unknown. We studied benzastatin biosynthetic gene cluster Streptomyces sp. RI18; functions six enzymes encoded it were analyzed disruption in a heterologous host vitro enzyme assays. propose pathway for benzastatins which cytochrome P450 (BezE) is responsible...
The HLA-Cw6 antigen has been associated with psoriasis vulgaris despite racial and ethnic differences. However, it remains unclear whether is the itself or a closely linked, hitherto unidentified, locus that predisposes to disease. Here, in order map susceptibility for precisely within HLA class I region, 11 polymorphic microsatellite markers distributed throughout 1060 kb segment surrounding HLA-C were subjected association analysis Japanese patients. Statistical analyses of distribution...
We recently identified a trinucleotide repeat polymorphism, (GCT)n, within the transmembrane (TM) segment of human MHC class I MICA gene (MHC chain‐related A). Five distinct alleles (A4, A5, A5.1, A6, A9) corresponding to 4, 5, 5 with one nucleotide insertion, 6 and 9 repetitions, respectively, have been detected in various HLA‐homozygous B cell lines. Here we present allele frequencies for this trimeric short tandem (STR) 604 unrelated individuals collected from nine populations (Japanese,...
Curcuminoids are pharmaceutically important compounds isolated from the herb Curcuma longa . Two additional type III polyketide synthases, named CURS2 and CURS3, that capable of curcuminoid synthesis were identified characterized. In vitro analysis revealed preferred feruloyl‐CoA as a starter substrate CURS3 both p ‐coumaroyl‐CoA. These results suggested synthesizes curcumin or demethoxycurcumin curcumin, bisdemethoxycurcumin demethoxycurcumin. The availability substrates expression levels...
Griselimycins (GMs) are depsidecapeptides with superb anti-tuberculosis activity. They contain up to three (2S,4R)-4-methyl-prolines (4-MePro), of which one blocks oxidative degradation and increases metabolic stability in animal models. The natural congener this substitution is only a minor component fermentation cultures. We showed that product can be significantly increased by feeding the reaction 4-MePro we investigated molecular basis biosynthesis incorporation. identified GM...
Total control: The key steps in the biosynthesis of elansolide A1, a new and structurally unique polyketide metabolite from gliding bacterium Chitinophaga sancti, have been elucidated feeding experiments, by analysis biosynthetic gene cluster, through synthesis model substrates. These include an unprecedented dehydration reaction, intramolecular Diels–Alder cycloaddition (IMDA), unusual macrolactonization. Detailed facts importance to specialist readers are published as "Supporting...
Curcuminoids, major components of the spice turmeric, are used as a traditional Asian medicine and food additive. Curcumin, representative curcuminoid, has received great deal attention because its anti-inflammatory, anticarcinogenic, antitumor activities. Here we report novel type III polyketide synthase named curcuminoid from Oryza sativa, which synthesizes bisdemethoxycurcumin via unique mechanism two 4-coumaroyl-CoAs one malonyl-CoA. The reaction begins with thioesterification thiol...
Curcuminoids, which are produced specifically by plants of the order Zingiberales, have long been used as food additives because their aromatic, stimulant and colouring properties traditional Asian medicines anti-tumour, antioxidant hepatoprotective activities. Curcuminoids therefore attractive targets for metabolic engineering. An artificial curcuminoid biosynthetic pathway, including reactions phenylalanine ammonia-lyase (PAL) from yeast Rhodotorula rubra, 4-coumarate : CoA ligase (4CL)...
Abstract Genome mining is a powerful method for finding novel secondary metabolites. In our study on the biosynthetic gene cluster cyclic octapeptides surugamides A–E (inhibitors of cathepsin B), we found putative consisting four successive non‐ribosomal peptide synthetase (NRPS) genes, surA , surB surC and surD . Prediction amino acid sequence based NRPSs inactivation revealed that are produced by two NRPS which were separated The latter genes responsible biosynthesis an unrelated peptide,...
Cardiac sarcoidosis occurs in 1–5% of patients. We previously reported a significant increase the uncommon TNFA (tumor necrosis factor α) allele, TNFA2 with cardiac Japanese. In order to precisely localize susceptible locus for within HLA region, genetic polymorphisms classical genes, non‐classical class II genes such as HLA‐DMA and ‐DMB several involved I‐mediated antigen presentation pathway (TAP1, TAP2, LMP2 LMP7) were investigated. Further, association analyses using four polymorphic...
Cytochrome P450 monooxygenases (P450s), which constitute a superfamily of heme-containing proteins, catalyze the direct oxidation variety compounds in regio- and stereospecific manner; therefore, they are promising catalysts for use oxyfunctionalization chemicals. In course our comprehensive substrate screening all 27 putative P450s encoded by Streptomyces griseus genome, we found that Escherichia coli cells producing an S. (CYP154C3), was fused C terminally with reductase domain (RED)...
Streptomyces sp. MSC090213JE08 seems to have more than 20 cryptic biosynthetic gene clusters for secondary metabolites. We aimed activate some of them by forced production antibiotic regulatory protein (SARP) family transcriptional activators. constructed seven recombinant strains, each which contained a SARP under the control constitutive promoter, and subjected comparative metabolic profiling analysis. Four strains produced nine metabolites that were hardly detected in strains. isolated...