A5003279242- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Fungal Biology and Applications
- Plant Disease Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Microbial Metabolism and Applications
- Insect-Plant Interactions and Control
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Tannin, Tannase and Anticancer Activities
- Asymmetric Synthesis and Catalysis
- Extracellular vesicles in disease
- Toxin Mechanisms and Immunotoxins
- Studies on Chitinases and Chitosanases
- Agriculture, Plant Science, Crop Management
- Allelopathy and phytotoxic interactions
- Cancer Treatment and Pharmacology
- Insect and Pesticide Research
- Plant tissue culture and regeneration
- Natural product bioactivities and synthesis
Biologie et Gestion des Risques en Agriculture
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université Paris-Saclay
2016-2024
Institut de Recherche en Horticulture et Semences
2023
L'Institut Agro
2023
Institut de Chimie des Substances Naturelles
2019-2022
AgroParisTech
2016-2020
Abstract The sessile nature of plants forced them to evolve mechanisms prioritize their responses simultaneous stresses, including colonization by microbes or nutrient starvation. Here, we compare the genomes a beneficial root endophyte, Colletotrichum tofieldiae and its pathogenic relative C. incanum , examine transcriptomes both fungi plant host Arabidopsis during phosphate Although two species diverged only 8.8 million years ago have similar gene arsenals, identify genomic signatures...
Abstract Specialized or secondary metabolites are small molecules of biological origin, often showing potent activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis these natural products is governed by sets co-regulated physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs a standardized machine-readable way, Minimum Information Biosynthetic Gene cluster (MIBiG) data standard repository was initiated...
The ascomycete fungus Colletotrichum higginsianum causes anthracnose disease of brassica crops and the model plant Arabidopsis thaliana. Previous versions genome sequence were highly fragmented, causing errors in prediction protein-coding genes preventing analysis repetitive sequences architecture. Here, we re-sequenced using single-molecule real-time (SMRT) sequencing technology and, combination with optical map data, this provided a gapless assembly all twelve chromosomes except for...
The genome of the hemibiotrophic anthracnose fungus, Colletotrichum higginsianum, encodes a large inventory putative secreted effector proteins that are sequentially expressed at different stages plant infection, namely appressorium-mediated penetration, biotrophy and necrotrophy. However, destinations to which these addressed inside cells unknown. In present study, we selected 61 genes highly induced in appressoria and/or biotrophic hyphae. We then used Agrobacterium-mediated transformation...
Colletotrichum destructivum ( Cd ) is a phytopathogenic fungus causing significant economic losses on forage legume crops Medicago and Trifolium species) worldwide. To gain insights into the genetic basis of fungal virulence host specificity, we sequenced genome an isolate from sativa using long-read (PacBio) technology. The resulting assembly has total length 51.7 Mb comprises ten core chromosomes two accessory chromosomes, all which were telomere to telomere. A 15, 631 gene models...
Colletotrichum higginsianum is an ascomycete fungus causing anthracnose disease on numerous cultivated plants in the family Brassicaceae, as well model plant Arabidopsis thaliana We report assembly of nuclear genome and gene annotation this pathogen, which was obtained using a combination PacBio long-read sequencing optical mapping.
Abstract Fungal phytopathogens secrete extracellular vesicles (EVs) associated with enzymes and phytotoxic metabolites. While these are thought to promote infection, defining the true contents functions of fungal EVs, as well suitable protein markers, is an ongoing process. To expand our understanding EVs their possible roles during we purified from hemibiotrophic phytopathogen Colletotrichum higginsianum , causative agent anthracnose disease in multiple plant species, including Arabidopsis...
Summary The role of histone 3 lysine 4 (H3K4) methylation is poorly understood in plant pathogenic fungi. Here, we analysed the function CclA, a subunit COMPASS complex mediating H3K4 methylation, brassica anthracnose pathogen Colletotrichum higginsianum . We show that CclA required for full genome‐wide trimethylation. deletion cclA strongly reduced mycelial growth, asexual sporulation and spore germination but did not impair morphogenesis specialized infection structures (appressoria...
Infection of Arabidopsis thaliana by the ascomycete fungus Colletotrichum higginsianum is characterized an early symptomless biotrophic phase followed a destructive necrotrophic phase. The fungal genome contains 77 secondary metabolism-related biosynthetic gene clusters, whose expression during infection process tightly regulated. Deleting CclA, chromatin regulator involved in repression some clusters through H3K4 trimethylation, allowed overproduction three families terpenoids and isolation...
Colletotrichum higginsianum is the causal agent of crucifer anthracnose disease, responsible for important economic losses in Brassica crops. A mutant lacking CclA subunit COMPASS complex was expected to undergo chromatin decondensation and activation cryptic secondary metabolite biosynthetic gene clusters. Liquid-state fermentation ΔcclA coupled with situ solid-phase extraction led production three families compounds, namely, colletorin colletochlorin derivatives two new representatives, D...
Abstract We report here a new application, CustomProteinSearch (CusProSe), whose purpose is to help users search for proteins of interest based on their domain composition. The application customizable. It consists two independent tools, IterHMMBuild and ProSeCDA. allows the iterative construction Hidden Markov Model (HMM) profiles conserved domains selected protein sequences, while ProSeCDA scans proteome against an HMM profile database, annotates identified using user-defined rules....
Discovering new solutions for crop protection is a major challenge the next decades as result of ecotoxicological impact classical fungicides, emergence fungicide resistances, and consequence climate change on pathogen distribution. Previous work fungal mutants deficient in unfolded protein response (UPR) supported that targeting this pathway promising plant disease control strategy. In particular, we showed UPR involved virulence by altering cell against host defense compounds, such...
Colletotrichum higginsianum is a hemibiotrophic pathogen that causes anthracnose disease on crucifer hosts, including Arabidopsis thaliana. Despite the availability of genomic and transcriptomic information ability to transform both organisms, identifying C. genes involved in virulence has been challenging due recalcitrance gene targeting redundancy factors. To overcome these obstacles, we developed an efficient method for multiple disruption by combining CRISPR/Cas9 URA3-based marker...
Transcriptomic studies have revealed that fungal pathogens of plants activate the expression numerous biosynthetic gene clusters (BGC) exclusively when in presence a living host plant. The identification and structural elucidation corresponding secondary metabolites remain challenging. aim was to develop polycistronic system for heterologous BGCs Saccharomyces cerevisiae. Here we adapted vector efficient, seamless cost-effective cloning genes using vivo assembly (also called...
Abstract Colletotrichum destructivum ( Cd ) is a phytopathogenic fungus causing significant economic losses on forage legume crops Medicago and Trifolium species) worldwide. To gain insights into the genetic basis of fungal virulence host specificity, we sequenced genome an isolate from M. sativa using long-read (PacBio) technology. The resulting assembly has total length 51.7 Mb comprises 10 core chromosomes two accessory chromosomes, all which were telomere to telomere. A 15,631 gene...
Abstract Infection of Arabidopsis thaliana by the ascomycete fungus Colletotrichum higginsianum is characterised an early symptomless biotrophic phase followed a destructive necrotrophic phase. The fungal genome contains 77 secondary metabolism-related biosynthetic gene clusters (BGCs), and their expression during infection process tightly regulated. Deleting CclA , chromatin regulator involved in repression some BGCs through H3K4 trimethylation, allowed overproduction 3 families terpenoids...
Abstract Fungal phytopathogens secrete extracellular vesicles (EVs) associated with enzymes and phytotoxic metabolites. While these are thought to promote infection, defining the true contents functions of fungal EVs, as well suitable protein markers, is an ongoing process. To expand our understanding EVs their possible roles during we purified from hemibiotrophic phytopathogen Colletotrichum higginsianum , causative agent anthracnose disease in multiple plant species, including Arabidopsis...
Abstract Colletotrichum higginsianum is a hemibiotrophic pathogen that causes anthracnose disease on crucifer hosts, including Arabidopsis thaliana. Despite the availability of genomic and transcriptomic information ability to transform both organisms, identifying C. genes involved in virulence has been challenging due their recalcitrance gene targeting redundancy factors. To overcome these obstacles, we developed an efficient method for multiple disruption by combining CRISPR-Cas9...
Abstract Transcriptomic studies have revealed that fungal pathogens of plants activate the expression numerous biosynthetic gene clusters (BGC) exclusively when in presence a living host plant. The identification and structural elucidation corresponding secondary metabolites remain challenging. Here we adapted polycistronic vector for efficient, seamless cost-effective cloning genes using vivo assembly (also called transformation-assisted recombination) directly Escherichia coli followed by...