A5000497684- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Cell death mechanisms and regulation
- Biochemical and Molecular Research
- Pancreatic function and diabetes
- PI3K/AKT/mTOR signaling in cancer
University of Victoria
2022-2024
Class IB phosphoinositide 3-kinase (PI3Kγ) is activated in immune cells and can form two distinct complexes (p110γ-p84 p110γ-p101), which are differentially by G protein-coupled receptors (GPCRs) Ras. Using a combination of X-ray crystallography, hydrogen deuterium exchange mass spectrometry (HDX-MS), electron microscopy, molecular modeling, single-molecule imaging, activity assays, we identify differences between p110γ-p84 p110γ-p101 that explain their differential membrane recruitment...
The formation of complexes between Rab11 and its effectors regulates multiple aspects membrane trafficking, including recycling ciliogenesis. WD repeat–containing protein 44 (WDR44) is a structurally uncharacterized effector that ciliogenesis by competing with prociliogenesis factors for binding. Here, we present detailed biochemical biophysical characterization the WDR44–Rab11 complex define specific residues mediating Using AlphaFold2 modeling hydrogen/deuterium exchange mass spectrometry,...