A5076171687- Malaria Research and Control
- Mosquito-borne diseases and control
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- Neonatal Health and Biochemistry
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Computational Drug Discovery Methods
- Insect and Pesticide Research
- HIV Research and Treatment
- Blood properties and coagulation
- Invertebrate Immune Response Mechanisms
- Genetic and Environmental Crop Studies
- Pharmacogenetics and Drug Metabolism
- Glaucoma and retinal disorders
- Neutropenia and Cancer Infections
- Blood transfusion and management
- Vitamin K Research Studies
- Advanced biosensing and bioanalysis techniques
- Glutathione Transferases and Polymorphisms
- Retinal and Macular Surgery
University of Turin
2016-2025
Accademia Albertina delle Belle Arti
2023-2024
Stockholm University
2017-2018
Pediatrics and Genetics
2010
National Medical Research Center for Hematology
1999-2004
Acute and chronic Plasmodium falciparum malaria are accompanied by severe immunodepression possibly related to subversion of dendritic cells (DC) functionality. Phagocytosed hemozoin (malarial pigment) was shown inhibit monocyte functions immunity. Hemozoin-loaded monocytes, frequently found in circulation adherent endothelia malaria, may interfere with DC development play a role immunodepression. unloaded human monocytes were differentiated vitro immature (iDC) treatment GM-CSF IL-4, mature...
The first human transfusion was performed by the pioneer Dr Jean-Baptiste Denis in France 1667 and now, three centuries later, around 50 millions blood units are transfused every year, saving of lives. Today, there is a new application for red cells (RBCs) cellular therapy: effective use erythrocytes as vehicles chemical or biological drugs. Using this approach, therapeutic index RBC-entrapped molecules can be significantly improved with increased efficacy reduced side effects. This...
Objective: Malaria remains an important life-threatening disease that provokes a complex immune response, marked by initial boost followed long-term suppression, warranting further study. One of the manifestations compromised response is co-infections, which are frequently reported in malaria patients and post-malaria convalescent individuals. Monocyte motility crucial step cell recruitment, but this process not fully efficient malaria. Malarial pigment hemozoin, avidly phagocytosed...
Dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEA-S), which are the most abundant hormones secreted by adrenal cortex present in plasma at approximately 6 micro M, as well their analogue, 16alpha-bromoepiandrosterone (EPI), exerted antimalarial activities against two chloroquine-sensitive Plasmodium falciparum strains (Palo Alto, 50% inhibitory concentration [IC(50)] of EPI, 4.8 +/- 0.68 M; T996/86, IC(50) 7.5 0.91 DHEA-S, 19 2.6 M) one mildly chloroquine-resistant strain (FCR-3, 6.5 1.01...
Key Points Inhibitors of human Syk kinase suppress parasite egress. inhibitors prevent the tyrosine phosphorylation band 3 in P falciparum parasitized red blood cells, reducing release microparticles.
Insects vastly outnumber us in terms of species and total biomass, are among the most efficient voracious consumers plants on planet. As a result, to preserve crops, one primary tasks agriculture has always been need control reduce number insect pests. The current use chemical insecticides leads accumulation xenobiotics ecosystems decreased those ecosystems, including insects. Sustainable development human society is impossible without useful insects, so pests must be effective selective at...
Summary Severe anaemia is a life‐threatening complication of falciparum malaria associated with loss predominantly non‐parasitized red blood cells (npRBCs). This poorly elucidated process might be influenced by (i) rosettes, i.e. npRBCs cytoadherent to haemozoin‐containing parasitized RBCs (pRBCs) and (ii) generation in pRBCs 4‐hydroxynonenal (4‐HNE) through haemozoin‐catalysed lipid peroxidation. We explored whether close proximity rosettes may facilitate 4‐HNE transfer npRBCs, which likely...
Summary Converging in vitro evidence and clinical data indicate that oxidative stress may play important roles P lasmodium falciparum malaria, notably the pathogenesis of severe anaemia. However, modifications red blood cell ( RBC )‐membrane by 4‐hydroxynonenal (4‐ HNE ) haemoglobin‐binding, previously hypothesized to contribute mechanistically have not yet been tested for significance. In 349 non‐immune M ozambican newborns recruited a double‐blind placebo‐controlled chemoprophylaxis trial,...
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Enhanced production of nitric oxide (NO) following upmodulation the inducible isoform NO synthase (iNOS) by haemozoin (HZ), inflammatory cytokines and LPS may provide protection against Plasmodium falciparum malaria killing hepatic blood forms parasites inhibiting cytoadherence parasitized erythrocytes (RBC) to endothelial cells. Monocytes macrophages are considered contribute importantly protective upregulation iNOS NO. Data obtained with murine phagocytes fed human HZ synthetic (sHZ)...
Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), LdMNPV-free larvae, were treated oligoDNA to the RING (really interesting new gene) domain LdMNPV IAP-3 gene. The...
Post-translational modifications (PTMs) are essential for regulating protein functions, influencing various fundamental processes in eukaryotes. These include, but not limited to, cell signaling, trafficking, the epigenetic control of gene expression, and cycle, as well proliferation, differentiation, interactions between cells. In this review, we discuss PTMs that play a key role malaria parasite biology its pathogenesis. Phosphorylation, acetylation, methylation, lipidation lipoxidation,...
Abstract In falciparum malaria, rupture of parasitized RBC liberates hemozoin (HZ), polymerized heme that contains and generates lipoperoxidation products. HZ HZ‐loaded monocytes 4‐HNE attained approx. 50 15 μM, respectively. are precursors dendritic cells (DC). Here, the role as inhibitor DC differentiation was examined. in quantified after derivatization by HPLC. were differentiated vitro from human supplemented with GM‐CSF/IL‐4 analyzed for surface antigens 4‐HNE‐adducts FACScan labelling...
AbstractAbstractIn Plasmodium falciparum-parasitized erythrocytes, hemozoin (HZ) formation was accompanied by enhanced of 4-hydroxynonenal (HNE)–protein adducts on the cell surface, reaching in HZ-rich schizont forms 16.8-fold amount control non-parasitized cells. The addition 1–100 μM exogenous HNE to cells generated HNE-adducts surface proteins amounts similar those found schizonts. Parasitized as well HNE-treated erythrocytes showed decreased deformability (measured filterability through...
Abstract Background Severe falciparum malaria anaemia (SMA) is a frequent cause of mortality in children and pregnant women. The most important determinant SMA appears to be the loss non-parasitized red blood cells (np-RBCs) excess parasitized (p-) RBCs at schizogony. Based on data from acute where excretion haemoglobin urine increased plasma represented respectively less than 1% 0.5% total Hb loss, phagocytosis predominant mechanism removal np- p-RBC. Estimates indicate that np-RBCs are...