Ocular and cutaneous melanomas arose in new inbred lines of transgenic mice having an integrated recombinant gene comprised the tyrosinase promoter, expressed pigment cells, simian virus 40 early-region transforming sequences. The tumors were hypomelanotic histopathologically similar to corresponding human melanomas. Eye often originated at a young age, chiefly from retinal epithelium, also choroid, rarely ciliary body. eye grew aggressively, highly invasive, metastasized local distant...

The Gfi-1 protooncogene encodes a nuclear zinc-finger protein that carries novel repressor domain, SNAG, and functions as position- orientation-independent active transcriptional repressor. allows interleukin 2 (IL-2)-dependent T cells to escape G 1 arrest induced by IL-2 withdrawal in culture collaborates with c- myc pim-1 for the induction of retrovirus-induced lymphomas animals. Here we show overexpression also inhibits cell death cultivation IL-2-dependent T-cell lines IL-2-deficient...

ABSTRACT We have assessed the importance of a melanocyte-specific DNase I hypersensitive site and matrix attachment region situated 15 kb upstream mouse tyrosinase gene by analysis in transgenic mice. Transgenes containing all, part, or none this linked to promoter human cDNA were introduced into genetically albino mice, pigmentation transgene message levels analyzed resulting lines. The effect was enhance significantly expression melanocytes, provide position-independent transgene. Two...

Abstract The chinchilla ‐ mottled ( c m ) mutation at the mouse tyrosinase‐encoding locus leads to a transversely striped pattern of dark‐ and light‐grey coat colors in homozygotes. same basic occurs various other genotypes has previously been found represent clonal developmental history melanocytes. In homozygote such as / , cis ‐acting mechanisms would be expected account for color differences. To search these mechanisms, genomic structure was examined compared with wild‐type, its function...

A survey in 2000 to detect methicillin-resistant Staphylococcus aureus (MRSA) colonization Vancouver downtown east side injection drug users (IDUs) revealed an MRSA nasal incidence of 7.4%. This is a follow-up study determine the current prevalence and further characterize isolates risk factors for colonization. In this point carriage among IDUs, swabs were cultured S. aureus. Isolates studied their antimicrobial susceptibility patterns presence mecA Panton-Valentine leukocidin (PVL) genes...

Melanosis was found to various extents in a wide array of tissues all 23 autopsied mice whose transgene consisted the tyrosinase promoter fused simian virus 40 early-region oncogenic sequences. Pigmentation given animal attributable any or following; an increase numbers some normally pigmented cells neural crest origin (a result compatible with early stages transformation); elicitation melanin synthesis that have little melanin, none at (the latter possibly signaling metaplasia); unusual...

The W/Kit mouse locus, affecting proliferation and survival of pigment cells, blood germ is known to encode a tyrosine kinase growth factor receptor considered protooncogene; yet it has not heretofore been causally implicated in any malignancies those cells. Wf/Wf mutant coat comprises viable inviable melanoblast clones, seen ultimately as pigmented white transverse stripes--the latter more prominent. Judging from the pattern, all clones initially expand, ones then undergo programmed cell...

An early consensus in the ongoing discourse about graduate student preparation for diverse careers was that graduates lacked competencies relevant to non-academic professional settings. Lists of missing “skills” were developed universities and agencies sought address, most commonly by offering generic (transferable) skills workshops or courses. In this paper, we critique framing issue discuss limitations common approaches taken address it. We propose a more integrated approach, where...

A distal upstream regulatory element of the mouse tyrosinase gene has locus control region (LCR)-like activity and is required for position-independent expression linked genes. It consists a DNAse I hypersensitive site, which enhancer in neural crest-derived melanocytes, embedded within scaffold/matrix attachment (S/MAR), both are necessary LCR activity. To address role S/MAR expression, we assessed ability fragment containing most to insulate transgene from position effects. The sequence...

This case describes a young non-pregnant cat that presented with uterine prolapse in association an unusual diffuse, polypoid, fibrosing perimetritis and parametritis. Following ovariohysterectomy the recovered fully. No intra-abdominal complications were seen on ultrasound examination 3 months postsurgery. At time of writing, remains healthy.Uterine is relatively rare usually associated periparturient period. Inflammatory polypoid parametritis have not previously been documented cats, dogs...

To study the relative sensitivity of rat tracheal epithelial and mesothelial cell DNA to oxidant damage, we used comet assay, a gel microelectrophoresis method that allows visual determination strand breaks on cell-by-cell basis, evaluate damage after hydrogen peroxide exposure. By both qualitative quantitative cells demonstrated similar dose-response increase in number showing per exposure increasing concentrations peroxide; but even at highest concentration, some failed show damage....

The proliferation of cultured rat Nb2 lymphoma cells is dependent on prolactin (PRL) acting as the principal growth factor. Previously, PRL-independent sublines were obtained by PRL starvation parent line and cloning surviving cells. Development independence was in some cases found to be associated with a reciprocal translocation involving chromosome 14 at breakpoint 14p22. In present study, novel, 14p22 zinc finger protein-encoding gene, Gfi-1, has been examined for role cell proliferation....

The SELH/Bc inbred mouse stock has produced an unusually high number of spontaneous mutations, including sph 2Bc , nu Bc a recessive lens opacity, and three mutations at the c locus. Classical genetic molecular studies were done to investigate origin albino locus mutations. Southern blots probed with tyrosinase cDNA showed that two are deletions exons 1, 2 3. third mutation, 3Bc disruption, either rearrangement or insertion, in region exon 1. deletion mutation is anticipated be large as...

Four isolates of Candida albicans recovered from the blood and cerebral spinal fluid a 66-year-old man during episodes systemic infection separated by 3 months antifungal therapy were analyzed variety molecular typing methods. All four shown to represent same strain, indicating relapse rather than reinfection.