A5003268915- Atherosclerosis and Cardiovascular Diseases
- Mast cells and histamine
- Nuclear Receptors and Signaling
- Cytokine Signaling Pathways and Interactions
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Single-cell and spatial transcriptomics
- Inflammasome and immune disorders
- Autoimmune Bullous Skin Diseases
- Kawasaki Disease and Coronary Complications
- Cardiovascular Health and Disease Prevention
- Chronic Lymphocytic Leukemia Research
- Cerebrovascular and Carotid Artery Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- T-cell and B-cell Immunology
- Eosinophilic Disorders and Syndromes
Centre for Human Drug Research
2022-2024
Leiden University
2022-2024
Wihuri Research Institute
2022
Abstract Atherosclerosis is a lipid-driven chronic inflammatory disease; however, whether it can be classified as an autoimmune disease remains unclear. In this study, we applied single-cell T cell receptor seqencing (scTCR-seq) on human carotid artery plaques and matched peripheral blood mononuclear samples to assess the extent of TCR clonality antigen-specific activation within various subsets. We observed highest degree plaque-specific clonal expansion in effector CD4 + cells, these...
Calcification, a key feature of advanced human atherosclerosis, is positively associated with vascular disease burden and adverse events. We showed that macrocalcification can be stabilizing factor for carotid plaque molecular biology, due to inverse association immune processes. Mast cells (MCs) are important contributors instability, but their relationship unexplored. With hypothesis MC activation negatively associates macrocalcification, we aimed investigate the link between MCs...
Atherosclerosis is characterized by the accumulation of lipids and immune cells, including mast cells B in arterial wall. Mast contribute to atherosclerotic plaque growth destabilization upon active degranulation. The FcεRI-IgE pathway most prominent cell activation route. Bruton's Tyrosine Kinase (BTK) involved FcεRI-signaling may be a potential therapeutic target limit atherosclerosis. Additionally, BTK crucial development B-cell receptor signaling. In this project, we aimed assess effects...
Abstract Mast cells have been associated with the progression and destabilization of advanced atherosclerotic plaques. Reducing intraplaque mast cell accumulation upon atherosclerosis could be a potent therapeutic strategy to limit plaque destabilization. Leukotriene B 4 (LTB ) has reported induce chemotaxis in vitro. Here, we examined whether antagonism LTB -receptor BLT1 inhibit atherosclerosis. Expression genes involved biosynthesis was determined by single-cell RNA sequencing human...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Dutch Heart Foundation Background/Introduction Atherosclerosis is the underlying pathology many cardiovascular diseases and characterized by chronic inflammation in larger arteries. Activation NLRP3 inflammasome one drivers during atherosclerosis. Purpose Therefore, we aimed to determine ability novel bispecific anti-NLRP3 antibody, called InflamAb, inhibit Methods Results In vitro treatment bone marrow...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Dutch Heart Foundation Aging is an independent and dominant risk factor for atherosclerosis associated with a low-grade chronic inflammation termed inflammaging. This age-induced pro-inflammatory environment may have great impact on plaque-residing immune cells, including mast cells. Mast cells been found to accumulate in the human atherosclerotic plaque upon disease progression instability. However, it...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Dutch Heart Foundation Atherosclerosis is characterized by the accumulation lipids and immune cells in arterial wall it main underlying pathology causing cardiovascular diseases (CVD). Cytokines are involved all stages atherosclerosis most cytokines contribute to disease progression. For instance, IL-6 cytokine family members, such as IL-6, oncostatin-M cardiotrophin-1, closely related CVDs. However, role...
Mast cell-derived heparin proteoglycans (HEP-PG) can be mimicked by bioconjugates carrying antithrombotic and anti-inflammatory properties. The dual antiplatelet anticoagulant (APAC) construct administered, either locally or intravenously (i.v.), targets activated endothelium, its adhesion molecules, subendothelial matrix proteins, all relevant to atherogenesis. We hypothesized that APAC influences cellular interactions in atherosclerotic lesion development studied treatment during the...
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Dutch Heart Foundation Introduction and aim: Mimics mast cell-derived heparin proteoglycans (HEP-PG) can be tailored to molecules carrying both antiplatelet anticoagulant properties. These dual (APAC) constructs also shield adhesion such as P-selectin VCAM-1 expressed by endothelial cells upon atherosclerosis development. We hypothesize that via this way, APAC prevents...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Dutch Heart Foundation Aim Acute cardiovascular diseases, such as myocardial infarction or stroke, are still a major cause death in Western Society. The main underlying pathology diseases is atherosclerosis, which caused by the accumulation lipids and inflammatory cells vessel wall, so-called atherosclerotic plaques. Mast accumulate within these plaques activation mast leads to progression destabilization...