A5047606458- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Advanced MRI Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Cardiac Imaging and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- CRISPR and Genetic Engineering
- Advanced NMR Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Cellular transport and secretion
Deutsches Diabetes-Zentrum e.V.
2020-2024
Heinrich Heine University Düsseldorf
2020-2024
German Center for Diabetes Research
2020-2024
Impaired proinsulin-to-insulin processing in pancreatic β-cells is a key defective step both type 1 diabetes and 2 (T2D) (refs.
The Rab-GTPase–activating protein (RabGAP) TBC1D4 (AS160) represents a key component in the regulation of glucose transport into skeletal muscle and white adipose tissue (WAT) is therefore crucial during development insulin resistance type 2 diabetes. Increased daily activity has been shown to be associated with improved postprandial hyperglycemia allele carriers loss-of-function variant human gene. Using conventional Tbc1d4-deficient mice (D4KO) fed high-fat diet, we show that moderate...
The two closely related RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4 play a crucial role in the regulation of GLUT4 translocation response to insulin contraction skeletal muscle. In mice, deficiency one or both RabGAPs leads reduced insulin- contraction-stimulated glucose uptake elevated fatty acid (FA) oxidation glycolytic oxidative muscle fibers without altering mitochondrial copy number abundance for phosphorylation. Here we present evidence novel mechanism lipid utilization...
The two closely related RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4, both substrates for AMPK, play important roles in exercise metabolism contraction-dependent translocation of GLUT4 skeletal muscle. However, the specific contribution each RabGAP contraction signaling is mostly unknown. In this study, we investigated cooperative AMPK-RabGAP axis metabolic response to exercise/contraction using a novel mouse model deficient active muscle AMPK combined with knockout either...
Macrophages play a pivotal role in vascular inflammation and predict cardiovascular complications. Fluorine-19 magnetic resonance imaging (19F MRI) with intravenously applied perfluorocarbon allows background-free direct quantification of macrophage abundance experimental disease models mice. Recently, perfluorooctyl bromide-nanoemulsion (PFOB-NE) was to effectively image infiltration pig model myocardial infarction using clinical MRI scanners. In the present proof-of-concept approach, we...
<p dir="ltr">The RabGTPase-activating protein (RabGAP) TBC1D4 (=AS160) represents a key component in the regulation of glucose transport into skeletal muscle and white adipose tissue (WAT) is therefore crucial during development insulin resistance type-2 diabetes. Increased daily activity has been shown to be associated with improved postprandial hyperglycemia allele carriers loss-of-function variant human <i>TBC1D4</i> gene. Using conventional...
<p dir="ltr">The RabGTPase-activating protein (RabGAP) TBC1D4 (=AS160) represents a key component in the regulation of glucose transport into skeletal muscle and white adipose tissue (WAT) is therefore crucial during development insulin resistance type-2 diabetes. Increased daily activity has been shown to be associated with improved postprandial hyperglycemia allele carriers loss-of-function variant human <i>TBC1D4</i> gene. Using conventional...
Type 2 diabetes (T2D) represents a multifactorial metabolic disease with strong genetic predisposition. Despite elaborate efforts in identifying the variants determining individual susceptibility towards T2D, majority of factors driving development remain poorly understood. With aim to identify novel T2D risk genes we previously generated an N2 outcross population using two inbred mouse strains New Zealand obese (NZO) and C3HeB/FeJ (C3H). A linkage study performed this led identification...
Measurement of ATP concentrations and synthesis in humans indicated abnormal hepatic energy metabolism obesity, non‐alcoholic fatty liver disease (NAFLD) Type 2 diabetes. Further mechanistic studies on require the detailed phenotyping specific mouse models. Thus, this study aimed to establish evaluate a robust fast single voxel 31 P MRS method quantify γ‐ATP at 11.7 T three models with different insulin sensitivities fat contents (72‐week‐old C57BL/6 control mice, 72‐week‐old resistant...
The two closely related RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4 play a crucial role in the regulation of GLUT4 translocation response to insulin contraction skeletal muscle. In mice, deficiency one or both RabGAPs leads reduced contraction-stimulated glucose uptake, elevated fatty acid uptake oxidation glycolytic oxidative muscle fibers without altering mitochondrial copy number abundance OXPHOS proteins. Here we present evidence for novel mechanism lipid utilization...
The two closely related RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4, both substrates for the AMP-activated protein kinase AMPK, play important roles in exercise metabolism contraction-dependent translocation of glucose transporter GLUT4 skeletal muscle. However, specific contribution each RabGAP contraction signaling is mostly unknown. In this study, we investigated cooperative AMPK/RabGAP axis metabolic response to exercise/contraction using a novel mouse model deficient...
The two closely related RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4 play a crucial role in the regulation of GLUT4 translocation response to insulin contraction skeletal muscle. In mice, deficiency one or both RabGAPs leads reduced contraction-stimulated glucose uptake, elevated fatty acid uptake oxidation glycolytic oxidative muscle fibers without altering mitochondrial copy number abundance OXPHOS proteins. Here we present evidence for novel mechanism lipid utilization...
Abstract Early macrophage rich vascular inflammation is a key feature in the pathophysiology of restenosis after angioplasty. 19 F MRI with intravenously applied perfluorooctyl bromide-nanoemulsion (PFOB-NE) could offer ideal features for serial imaging inflammatory response We aimed to non-invasively image monocyte/macrophage infiltration angioplasty pig carotid arteries using Fluorine-19 magnetic resonance ( MRI) assess early mechanical injury. In eight minipigs, injury right artery was...
The two closely related RabGTPase-activating proteins (RabGAPs) TBC1D1 and TBC1D4, both substrates for the AMP-activated protein kinase AMPK, play important roles in exercise metabolism contraction-dependent translocation of glucose transporter GLUT4 skeletal muscle. However, specific contribution each RabGAP contraction signaling is mostly unknown. In this study, we investigated cooperative AMPK/RabGAP axis metabolic response to exercise/contraction using a novel mouse model deficient...