HMG-D is an abundant chromosomal protein associated with condensed chromatin during the first nuclear cleavage cycles of developing Drosophila embryo. We previously suggested that might substitute for linker histone H1 in preblastoderm embryo and this substitution result characteristic less compacted chromatin. have now studied association using a cell-free system reconstitution derived from embryos. Association chromatin, like H1, increases nucleosome spacing indicative binding to DNA...

Competent larvae of the black scallop Chlamys varia (L.) were treated with neurotransmitter gamma-amino-n-butyric acid (GABA), catecholamine epinephrine, and 3-isobutyl-1-methylxanthine (IBMX), at different concentrations times exposure, to test effects exposure compounds on larval settlement. After 24 h, maximum percentage settlement (>30%) was induced by 10−5 M epinephrine. 48 rate also achieved epinephrine (>54%). Exposure 10−6 GABA significantly higher than in control larvae. By...

The production of the flat oyster Ostrea edulis (L.) natural spat in Europe has decreased almost by 60% past ten years. Thus, importance hatcheries is evident. One critical steps hatchery broodstock conditioning, especially difficult autumn, when gonadal development resting period. Conditioning influence temperature, photoperiod and nutrition. In this work, effects two temperature three regimes on conditioning O. were studied for years stereological analyses registering number dates spawning...

Some species of the genus Pseudo-nitzschia produce toxin domoic acid, which causes amnesic shellfish poisoning (ASP). Given that bivalve mollusks are filter feeders, they can accumulate these toxins in their tissues. To elucidate transcriptional response queen scallop Aequipecten opercularis after exposure to acid-producing Pseudo-nitzschia, digestive gland transcriptome was de novo assembled using an Illumina HiSeq 2000 platform. Then, a differential gene expression analysis performed....

Blooms of toxic microalgae and the accumulation their compounds in bivalve molluscs are a regular worldwide problem to both consumers producers. To select populations that accumulate less amount toxins is very important understand metabolism biotoxins. The study gene expression patterns by real-time quantitative polymerase chain reaction (RT-qPCR) may provide insights into genes involved detoxification metabolic pathways. One critical steps using RT-qPCR results have be normalized internal...

Some diatom species of the genus Pseudo-nitzschia produce toxin domoic acid. The depuration rate acid in Pecten maximus is very low; for this reason, king scallops generally contain high levels their tissues. A transcriptomic approach was used to identify genes differentially expressed P. digestive gland after injection differential expression analysis found 535 (226 up-regulated and 309 down-regulated). Protein–protein interaction networks obtained with were enriched gene ontology terms,...

The mussel Mytilus galloprovincialis is one of the most important aquaculture species in Europe. Its main production problem accumulation toxins during coastal blooms, which prevents commercialization. P-glycoprotein (ABCB1/MDR1/P-gp) part multixenobiotic resistance system aquatic organisms, and okadaic acid, DSP toxin, probably a substrate P-gp-mediated efflux. In this study, presence possible role P-gp acid detoxification process was studied M. galloprovincialis. We identified, cloned,...

Abstract Compounds of type [MX 2 (Hpben)] [M = Pd (X Cl), Pt Cl, I); Hpben 2‐(2′‐pyridyl)benzimidazole] were prepared and characterized, the structures derivatives determined by X‐ray crystallography. The crystals [PtI consist discrete units in which atom is coordinated to two iodine atoms pyridine imidazole N a distorted square planar arrangement. structure chloro derivative similar, except that [PtCl monomers are hydrogen‐bonded zig‐zag chains. In assays interactions compounds with DNA,...

Multidrug resistance associated proteins (MRP) belong to the ABCC branch of ABC transporters. The MRP together with P-gp (P-glycoprotein; MDR1; ABCB1) and BCRP (breast cancer protein; ABCG2) confer multixenobiotic (MXR) in marine vertebrates. In aquatic invertebrates, little is known about presence role these transporters play an important absorption, distribution, excretion drugs, xenobiotics, endogenous compounds are predominantly expressed excretory organs. present study, we identified...

The flat oyster Ostrea edulis (Linnaeus, 1758) is a commercially valuable species in Europe. production of O. natural spat has decreased the past, and it insufficient to meet demand. Thus, importance hatcheries increasing. conditioning broodstock may be improved by manipulating several external factors such as temperature, food supply, photoperiod; however, few studies have reported effect these parameters on gametogenesis conditioning. In this report, combined effects temperature...

The natural production of the native flat oyster Ostrea edulis and seed in hatcheries are insufficient to meet demand. Several projects have recently been developed optimise establish a reliable protocol for broodstock conditioning hatcheries. In this sense, positive effect gradient temperature daylight on gonadal development, spawning larval O. during winter autumn was previously confirmed. No other study has yet optimised factors conditioning. This work examines combined two important...

To establish a reliable protocol for the broodstock conditioning of oyster Ostrea edulis (Linnaeus 1758) in hatcheries is important to obtain best number viable larvae all year even outside natural season maturation and spawning. Hatchery production bivalves depends on their initial gonadal state, which linked several other factors, such as temperature, daylight, salinity food supply. Ration diet composition are because availability affects fecundity gametogenic development. In this work,...

Molluscs belong to one of the two protostome superphyla, Lophotrochozoa. Among molluscs, bivalves show a characteristic shell morphology separated bilaterally into plates. The connection between modification development and evolution molluscan body plan can be study identifying obtaining information on genes that regulate bivalves. Homeobox are involved in formation regulation many developmental processes bilateria. For this reason, evolutionary history Hox, ParaHox, EHGbox, NK homeobox gene...