A5037602703- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Analytical chemistry methods development
- Antibiotics Pharmacokinetics and Efficacy
- Pharmacological Effects and Assays
- Protein Hydrolysis and Bioactive Peptides
- Algal biology and biofuel production
- Synthesis and Biological Evaluation
- Electrochemical sensors and biosensors
- Pharmaceutical and Antibiotic Environmental Impacts
- Indoor Air Quality and Microbial Exposure
- Advanced Biosensing Techniques and Applications
- Polyamine Metabolism and Applications
- Mycotoxins in Agriculture and Food
- Molecular Sensors and Ion Detection
- Breastfeeding Practices and Influences
- Advanced Nanomaterials in Catalysis
- Glycosylation and Glycoproteins Research
- Food Quality and Safety Studies
- Mangiferin and Mango Extracts
- Melamine detection and toxicity
- Exercise and Physiological Responses
- Medicinal Plants and Bioactive Compounds
- Carbon and Quantum Dots Applications
- Biochemical Acid Research Studies
Hebei University of Science and Technology
2020-2024
Tianjin University of Science and Technology
2015-2017
Ministry of Education of the People's Republic of China
2017
A novel fluorescence immunoassay for detecting sulfaquinoxaline (SQX) in animal-derived foods was developed using NaYF4:Yb/Tm upconversion nanoparticles (UCNPs) conjugated with antibodies as signal probes, and monodisperse magnetic polystyrene microspheres (MMPMs) modified coating antigen immune-sensing capture probes trapping separating the probes. Based on a competitive format, detection limit of proposed method SQX 0.1 μg L(-1) buffer 0.5 kg(-1) food samples. The recoveries spiked samples...
In this study, golden syrian hamsters were regarded as modeling objects, a series of sea buckthorn fruit oil (SBFO) extract, which is rich in palmitoleic acid (POA), was evaluated through vivo experiments to explore its mechanism action anti-hyperlipidemia. The results revealed that SBFO extract could control body weight and adipose tissue mass, relieve fat accumulation, together with improving total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C) non-high-density...
A competitive indirect enzyme-linked immunosorbent assay (ciELISA) using a polyclonal antibody was developed to detect tyramine in meat and seafood. This ciELISA had 50% inhibition concentration (IC50) of 0.20 mg/L limit detection (LOD) 0.02 showed no cross-reactivity with tyrosine or other biogenic amines. The average recoveries from spiked samples for this ranged 85.6 102.6%, the results exhibited good correlation high-performance liquid chromatography (HPLC) results. LOD seafood 1.20...
Irrational use of fluoroquinolones (FQs) can lead to allergic reactions, adverse reactions the heart and damage liver; thus, it is great significance establish rapid, sensitive accurate detection methods for FQs. Molecularly imprinted polymers (MIPs) with specific structures synthesized by molecular imprinting technology (MIT) are widely used FQs due their high specificity, sensitivity stable performance. Recently, new functional nanomaterials different morphologies sizes, which provide rich...
Quantum dot based fluorescence quenching immune chromatographic strips for simultaneous determination of sulfonamides and fluoroquinolones.
Antibiotic pollution has emerged as a serious problem in recent years. In this study, two novel fluorescence quenching biomimetic enzyme-linked immunosorbent assays (FQBELISAs) were constructed for the rapid detection of enrofloxacin animal-derived foods. strategy, quantum dots (QDs) was efficiently quenched by enzymatic formation horseradish peroxidase (HRP) and prussian blue nanoparticles (PBNPs) ELISA (BELISA). Under optimal conditions, limit (IC15) method using HRP 1.67 ng L-1,...
Abstract BACKGROUND To estimate the anti‐fatigue and antioxidative effects of water alcohol extracts from Diaphragma juglandis (DJ), H 2 O ‐treated HepG2 cells were used as an in vitro model to determine total antioxidant capacities these two extracts, behavioral tests on mice biochemical assay performed via vivo experiments. RESULTS The results indicate that both possess remarkable protective capable scavenging 2,2‐diphenyl‐1‐picrylhydrazyl 2,2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic...
Microwave technology has been widely used in the food industry, but effect of microwave-heated on human health is being questioned. Female KM mice were chosen to be treated with milk (MM), and reproductive markers such as litter size, birth rate, survival ovarian index evaluated. With longer term feeding, status (body weight, neonatal interpregnancy interval, brain superoxide dismutase catalase activity) MM did not significantly change except for first-generation mice, which was decreased...
Portable glucose meter (PGM) has played vital roles in the field of medicine, with advantages accurate and quantitative results, portability, easy operation, low cost. Recently, more researchers have extended application PGM detecting foods contaminations. In this paper, a review based on target recognition elements, such as antibody–antigen reaction, molecularly imprinted polymer, nucleic acid hybridization, enzyme recognition, click chemistry, amplification strategies food safety...
Schematic illustration of biomimetic fluorescence immunoassays based on QDs.
Abstract Background Lentinan can inhibit the proliferation of many kinds tumor cells, and some studies have shown that lentinan growth tissue by directly killing cells. The purpose this study was to investigate immunoprophylaxis effect mechanism specific antigen induced on liver cancer in mice. Methods immunogen prepared which lentinan. And two different mouse H22 models (ascites solid tumor)were established after immunized. immune organ index mice each group were measured. In order analyze...
The current study aimed to investigate the apoptotic and anti-proliferative effect of essential oils obtained from turmeric (Curcuma longa L.) at cellular level. To test effects mechanism, H1299 HepG2 cells were selected for in vitro experiments. Cytotoxicity assay morphology analyses performed estimate oil (TEO) on viability cell growth. Proapoptotic TEO investigated using flow cytometry. Subsequent experiments assessed migration colony formation evaluate anti-metastatic by wound-healing...
A highly sensitive and reliable fluorescence immunoassay based on the quenching (FQ) effect of quantum dots (QDs) was developed for detection norfloxacin (NOR) in animal-origin foods. Briefly, enzymatic formation horseradish peroxidase (HRP) conventional ELISA effectively quenched QDs through inner-filter (IFE) resonance energy transfer (FRET), then absorption signal transformed to fluorescent signals, resulting a higher sensitivity NOR foods.The limit this method 0.20 μg L-1 standard...