A5028468456- Pharmacogenetics and Drug Metabolism
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Drug Transport and Resistance Mechanisms
- Diabetes Treatment and Management
- Computational Drug Discovery Methods
- Statistical Methods in Clinical Trials
- Electrochemical sensors and biosensors
- Health Systems, Economic Evaluations, Quality of Life
- Nutrition, Genetics, and Disease
- Metabolism, Diabetes, and Cancer
- Hormonal and reproductive studies
- Migraine and Headache Studies
- Tuberculosis Research and Epidemiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Methods in Pharmaceuticals
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- Colorectal Cancer Treatments and Studies
- Treatment of Major Depression
- Psychedelics and Drug Studies
- Microfluidic and Capillary Electrophoresis Applications
- Forensic Toxicology and Drug Analysis
- Metabolism and Genetic Disorders
University of Castilla-La Mancha
2008-2025
Universidad de Extremadura
2012-2022
Centro de Implantología Cirugía Oral y Maxilofacial
2020-2022
Instituto de Investigación en Recursos Cinegéticos
2018-2020
Pharmacoeconomics and Health Outcomes Research Iberia (Spain)
2019
Universidad Complutense de Madrid
2012
Agencia Española de Medicamentos y Productos Sanitarios
2010
Gene expression data are accumulating exponentially in public repositories. Reanalysis and integration of themed collections from these studies may provide new insights, but requires further human curation. Here we report a crowdsourcing project to annotate reanalyse large number gene profiles Expression Omnibus (GEO). Through massive open online course on Coursera, over 70 participants 25 countries identify 2,460 single-gene perturbation signatures, 839 disease versus normal 906 drug...
Abstract Toxicity assays are commonly used as general indicators of environmental water pollution. In the study described here, selected toxicity tests have been to evaluate different levels enantiomers pharmaceutical drugs that can be found potential contaminants in environments. Isomers dopa, fluoxetine, and atenolol were tested with three aquatic organisms corresponding trophic levels: Daphnia magna (a crustacean), Pseudokirchneriella subcapitata microalga), Tetrahymena thermophila...
Abstract Depression is a common, severe, disabling mental disease that affects millions of people all ages worldwide. Various studies have shown neurotrophic/growth factors key role in depression and, more specifically, vascular endothelial growth factor (VEGF) implicated the pathogenesis depression. The purpose this study was to investigate potential links between four VEGF-related single-nucleotide polymorphisms (SNPs), previously identified through genome-wide association (GWAS) and...
Pharmacogenetic variation in Latin Americans is understudied, which sets a barrier for the goal of global precision medicine. The RIBEF-CEIBA Network Consortium was established to characterize interindividual and between population variations CYP2D6, CYP2C9, CYP2C19 drug metabolizing enzyme genotypes, were subsequently utilized catalog their "predicted metabolism phenotypes" across Native American Ibero populations. Importantly, we report this study, total 6060 healthy individuals from...
: Research on pharmacogenetic variability in response to prescribed drugs and across ethnic groups is essential for personalized medicine, particularly admixed unstudied populations. For the first time, this study examines
'Compassionate use' programmes allow medicinal products that are not authorised, but in the development process, to be made available patients with a severe disease who have no other satisfactory treatment them. We sought understand how such regulated ten European Union countries. The Clinical Research Infrastructures Network (ECRIN) conducted comprehensive survey on clinical research regulatory requirements, including questions regulations of 'compassionate programmes. Ten countries,...
Genetic variations within the cytochrome P450 (CYP450) superfamily of drug metabolizing enzymes confer substantial person-to-person and between-population differences in pharmacokinetics, by extension, highly variable clinical effects medicines. In this context, "personalized medicine," "precision "stratified medicine" are related concepts attributed to what is essentially targeted therapeutics companion diagnostics, aimed at improving safety effectiveness health interventions. We report...
Global precision medicine demands characterization of drug metabolism and phenotype variation in diverse populations, including the indigenous societies. A related question is extent to which CYP450 metabolizing enzyme genotype data are concordant whether they can be used interchangeably. These issues increasingly debated as continues expand a popular research topic worldwide. We report here first study clinically relevant phenotypes genotypes Mexican Amerindian subjects. In large sample 450...
Background: The analytical method here reported for the CEIBA cocktail approach has been developed and validated simultaneous determination of several probe drugs their corresponding cytochrome P450 (CYP) enzyme-specific metabolites in just one analysis. This methodology proposed order to overcome some drawbacks concerning complexity low throughput methodologies associated with previously approaches. Methods & results: Caffeine (CYP1A2), losartan (CYP2C9), omeprazole (CYP2C19),...
Abstract Poly(styrene‐co‐divinylbenzene)‐coated magnetic multiwalled carbon nanotube composite synthesized by in‐situ high temperature combination and precipitation polymerization of styrene‐co‐divinylbenzene has been employed as a sorbent for the solid phase extraction antidepressants in human urine samples. Fluoxetine, venlafaxine, citalopram sertraline were, afterwards, separated determined capillary electrophoresis with diode array detection. The presence nanotubes native...
In a one-way cross-over study, we investigated the effect of Khat, natural amphetamine-like psychostimulant plant, on catalytic activities five major drug-metabolizing cytochrome P450 (CYP) enzymes. After one-week Khat abstinence, 63 Ethiopian male volunteers were phenotyped using cocktail probe drugs (caffeine, losartan, dextromethorphan, omeprazole). Phenotyping was repeated after daily use 400 g fresh leaves. Genotyping for CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP3A5 done. Urinary cathinone...
The use of khat (Catha edulis) while on medication may alter treatment outcome. In particular, the influence metabolic activities drug-metabolizing enzymes is not known. We performed a comparative 1-way crossover study to evaluate effect cytochrome P450 (CYP)2D6 and CYP3A4 enzyme activity. After 1 week abstinence, baseline CYP2D6 were determined in 40 Ethiopian male volunteers using 30 mg dextromethorphan (DM) as probe drug then repeated after daily 400 g fresh leaves. Urinary concentrations...
Phenotyping of the CYP450 enzyme activities contributes to personalized medicine, but past phenotyping approaches have followed a piecemeal strategy measuring single in vivo. A barrier populations rural and remote areas is limited time resources for sample collection. The CEIBA cocktail approach allows metabolic capacity estimation multiple enzymes analysis, attendant collection schemes applications diverse global settings are yet be optimized. present study aimed select an optimal matrix...