A5100721184- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Plasmonic and Surface Plasmon Research
- Advanced biosensing and bioanalysis techniques
- Plant nutrient uptake and metabolism
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Sensors
- Water Quality Monitoring and Analysis
- Plant Molecular Biology Research
- Melamine detection and toxicity
- Plant Reproductive Biology
- Photonic and Optical Devices
- Plant Micronutrient Interactions and Effects
- E-commerce and Technology Innovations
- Identification and Quantification in Food
- Rice Cultivation and Yield Improvement
- Material Properties and Failure Mechanisms
- Advanced Chemical Sensor Technologies
- Traditional Chinese Medicine Analysis
- Food Quality and Safety Studies
- Molecular Junctions and Nanostructures
- Helminth infection and control
- Salmonella and Campylobacter epidemiology
- Parasite Biology and Host Interactions
- Plant Pathogenic Bacteria Studies
China National Rice Research Institute
2022-2024
Beijing Shijitan Hospital
2021
Zunyi Medical University
2021
Capital Medical University
2021
Yanbian University
2020
Henan Agricultural University
2005-2018
Institute of Botany
2014-2015
Chinese Academy of Sciences
2014-2015
Kunming University of Science and Technology
2014
Max Planck Society
2011
A biosensing system with optical fibers is proposed for the colorimetric detection of melamine in liquid milk samples by using localized surface plasmon resonance (LSPR) unmodified gold nanoparticles (AuNPs). The consists a broadband light source that covers spectral range from 200 nm to 1700 nm, an attenuator, three types 600 μm premium SMA905 connectors and miniature spectrometer linear charge coupled device (CCD) array. low-cost, simple well-proven melamine. Its working principle based on...
Ethylene as an indicator for evaluating fruit ripening can be measured by very sensitive electrochemical gas sensors based on a high-resolution current produced bias potential applied to the electrodes. For this purpose, measurement system monitoring ethylene concentrations evaluate using sensor was successfully developed. Before used measure released from fruits, calibration curve established standard gases at of 2.99 ppm, 4.99 8.01 ppm and 10 respectively, with flow rate 0.4 L·min(-1)....
Although aptamer-functionalized AuNPs technology exhibits great potential in analytical and biological chemistry, direct analysis of molecules at a low concentration using colorimetric assay remains challenging. The development intuitive methods has attracted interest for promising detection melamine milk samples due to demand stable understandable process. In this study, we propose rapid facile measurement method combined contaminated samples. To realize the high stability lower limit...
The purpose of this study was to develop a portable surface plasmon resonance (SPR) bioanalyzer for the sensitive detection Escherichia coli O157:H7 in comparison with an enzyme-linked immunosorbent assay (ELISA). experimental setup mainly consisted integrated biosensor and homemade microfluidic cell three-way solenoid valve. In order detect using SPR immunoassay, 3-mercaptopropionic acid (3-MPA) chemisorbed onto gold via covalent bond immobilization biological species....
Abscisic acid (ABA) plays an important role in abiotic stress response and physiological signal transduction resisting to the adverse environment. Therefore, it is very essential for quantitative detection of abscisic due its indispensable plant activities. Herein, a new method based on localized surface plasmon resonance (LSPR) using aptamer-functionalized gold nanoparticles (AuNPs) developed without expensive instrument antibody. In presence ABA, ABA specifically bind with their aptamers...
In Arabidopsis thaliana L., stomata are produced through a series of divisions including asymmetric and symmetric divisions. Asymmetric entry division meristemoid mother cell produces two daughter cells, the smaller larger sister cell, stomatal lineage ground (SLGC). Stomatal cells can differentiate into epidermal pavement but have potential to divide asymmetrically, spacing divisions, create satellite meristemoids. Peptide ligands TOO MANY MOUTHS (TMM) ERECTA family receptors regulate...
Lesion mimic mutants (LMMs) have been widely used in experiments recent years for studying plant physiological mechanisms underlying programmed cell death (PCD) and defense responses. Here, we identified a lesion mutant, lm212-1, which cloned the causal gene by map-based cloning strategy, verified this complementation. The gene, OsPHD1, encodes UDP-glucose epimerase (UGE), OsPHD1 was located chloroplast. constitutively expressed all organs, with higher expression leaves other green tissues....
A sensitive and label-free analytical approach for the detection of porcine circovirus type 2 (PCV2) instead PCV2 antibody in serum sample was systematically investigated this research based on surface plasmon resonance (SPR) with an establishment special molecular identification membrane. The experimental device constructing biosensing analyzer is composed integrated biosensor, a home-made microfluidic module, electrical control circuit incorporated photoelectric converter. In order to...
A surface plasmon resonance (SPR) immunoassay with an immobilization of self-assembled molecular identification membrane for the detection residual Clenbuterol Hydrochloride (CLB) in pork liver was systematically investigated and experimentally validated its high performance. SPR a regular competitive inhibition assay cannot be directly verified to detect CLB residuals. In this study, binding Au film mercaptopropionic acid using known form strong S-Au covalent bonds formed by chemical...
Kinetic analysis of biomolecular interactions are powerfully used to quantify the binding kinetic constants for determination a complex formed or dissociated within given time span. Surface plasmon resonance biosensors provide an essential approach in including interaction process antigen-antibody and receptors-ligand. The affinity antibody antigen (or receptor ligand) reflects biological activities control antibodies receptors) corresponding immune signal responses pathologic process....
The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This designed field use is characterized by special structure which consists microfluidic cell incorporating right angle prism functionalized with biomolecular identification membrane, laser line generator and data acquisition board. structure, memory capacity charge-coupled device (CCD) array driving collecting the photoelectric signals are...
Iron is an essential nutrient required for growth, development and also impacts disease resistance in plants. Plant absorb iron through their roots citrate plays a vital role transport of Fe 3+ the xylem. In this study, we found that rice cytoplasmic ATP-citrate lyase subunit A2, OsACL-A2 ( Os12g0566300) serves as crucial factor uptake translocation. Leaf levels were depressed osacl-a2 mutant which caused deficiency induced chlorosis, activated defense signaling eventually necrosis mature...
A novel data acquisition and analysis system has been developed for a surface plasmon resonance (SPR) bioanalyzer, which is based on the use of TSPR1k23 biosensor composed microflow cell, clamp, photoelectronic conversion device. virtual instrument consists computer-based platform that equipped with powerful application software cost-effective hardware, outperform functions obtaining from biosensor. In this system, The PIC24HJ32GP302 microprocessor interfacing 16bit ADS8326 analog-to-digital...
Background: Vitexin is the main bioactive compound of hawthorn (Crataegus pinnatifida), a famous traditional Chinese medicine, and vitexin for injection currently in phase I clinical trial China. Objective: This investigation systematically evaluated metabolism toxicokinetics rats dogs. Methods: Rats beagle dogs were administrated different doses vitexin, then plasma concentration, tissue distribution, excretion, metabolism, pharmacokinetics protein binding investigated. Results : The...
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