A5018806487- Epigenetics and DNA Methylation
- Zebrafish Biomedical Research Applications
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Corneal Surgery and Treatments
- Congenital heart defects research
- Ocular Surface and Contact Lens
- Hippo pathway signaling and YAP/TAZ
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Fibroblast Growth Factor Research
- Glaucoma and retinal disorders
- Genetic Syndromes and Imprinting
- Wnt/β-catenin signaling in development and cancer
- Lipid metabolism and biosynthesis
- Neuroinflammation and Neurodegeneration Mechanisms
- Corneal surgery and disorders
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
University of Hong Kong
2021-2024
Chinese University of Hong Kong
2024
Karlsruhe Institute of Technology
2021-2023
Dalian Medical University
2016-2021
Rationale: Malignant ascites in peritoneal metastases is a lipid-enriched microenvironment and frequently involved the poor prognosis of epithelial ovarian cancer (EOC). However, detailed mechanisms underlying (OvCa) cells dictating their lipid metabolic activities promoting tumor progression remain elusive. Methods: The omental conditioned medium (OCM) was established to imitate or microenvironment. Mass spectrometry, RT-qPCR, IHC, western blot assays were applied evaluate human fatty acid...
Abstract Refractive disorder is the most prevalent cause of visual impairment worldwide. While treatment refractive errors can bring improvement to quality life and socio-economic benefits, there a need for individualization, precision, convenience, safety with chosen method. Herein, we propose using pre-designed lenticules based on poly-NAGA-GelMA (PNG) bio-inks photo-initiated by digital light processing (DLP)-bioprinting correcting errors. DLP-bioprinting allows PNG have individualized...
Abstract Background In contrast to stable genetic events, epigenetic changes are highly plastic and play crucial roles in tumor evolution development. Epithelial ovarian cancer (EOC) is a heterogeneous disease that generally associated with poor prognosis treatment failure. Profiling epigenome-wide DNA methylation status therefore essential better characterize the impact of alterations on heterogeneity EOC. Methods An association study was conducted evaluate global retrospective cohort 80...
Motivation: DNA methylation is an important epigenetic modification that has essential role in gene regulation, cell differentiation and cancer development. Bisulfite sequencing a widely used technique to obtain genome-wide profiles, one of the key tasks analyzing bisulfite data detect differentially methylated regions (DMRs) among samples under different treatment conditions. Although numerous tools have been proposed single CpG site (DMC) between samples, methods for direct DMR detection,...
Peritoneal metastases are frequently found in high-grade serous carcinoma (HGSOC) patients and commonly associated with a poor prognosis. The tumor microenvironment (TME) is complex milieu that plays critical role epigenetic alterations driving development metastatic progression. However, the impact of on ovarian cancer cells harsh peritoneal remains incompletely understood. Here, we identified miR-33b silenced by promoter hypermethylation HGSOC derived from omental tissues. Enforced...
The central nervous system of adult zebrafish displays an extraordinary neurogenic and regenerative capacity. In the brain, this capacity relies on neural stem cells (NSCs) careful management NSC pool. However, mechanisms controlling pool maintenance are not yet fully understood. Recently, Bone Morphogenetic Proteins (BMPs) their downstream effector Id1 (Inhibitor differentiation 1) were suggested to act as key players in under constitutive conditions. Here, we further investigated role...
Peritoneal metastases frequently occur in epithelial ovarian cancer (EOC), resulting poor prognosis and survival rates. Tumor-associated-macrophages (TAMs) massively infiltrate into ascites spheroids are multi-polarized as protumoral M2-like phenotype, orchestrating the immunosuppression promoting tumor progression. However, impact of omental conditioned medium/ascites (OCM/AS) on TAM polarization its function progression remains elusive.
Sox transcription factors are crucial for vertebrate nervous system development. In zebrafish embryo, sox1 genes expressed in neural progenitor cells and neurons of ventral spinal cord. Our recent study revealed that the loss sox1a sox1b function results a significant decline V2 subtype (V2s). Using single-cell RNA sequencing, we analyzed transcriptome lineage progenitors cord at four time points during embryonic development, employing Tg(sox1a:eGFP) line. addition to previously...
The relationship between CpG content and DNA methylation has attracted considerable interest in recent years. Direct or indirect methods have been developed to investigate their regulatory functions based on various hypotheses, large cohort studies, meta-analyses. However, all of these analyses were performed at units blocks and, thus, the influence finer genome structure neglected. Herein, we present a novel algorithm base-pair resolution systematically contents methylation. By introducing...
Abstract The mechanisms of the management neural stem cell (NSC) pool underlying regenerative capacity adult zebrafish brain are not understood. We show that Bone Morphogenetic Proteins (BMPs) which exclusively expressed by neurons in telencephalon and helix-loop-helix (HLH) transcription co-regulator, Inhibitor differentiation 1 (Id1), control quiescence NSCs. Upon injury, lack id1 function leads to an initial over-proliferation subsequent loss NSCs capacity. BMP/Id1 signaling up-regulates...
Purpose: To study the in vitro effect of vitamin D3 on healing response human Tenon's fibroblasts (HTF) and its possible role preventing excessive postoperative subconjunctival fibrosis. Methods: Effect cytotoxicity cell survival primary cultured HTF was measured by lactate dehydrogenase PrestoBlue assays, respectively. Proliferation migration D3–treated (D3-HTF) determined CyQUANT proliferation scratch assay, The mRNA expression profiles control-HTF D3-HTF from six subjects (three with...
Abstract Malignant ascites in peritoneal metastases is a lipid-enriched microenvironment and frequently involved the poor prognosis of epithelial ovarian cancer (EOC). However, detailed mechanisms underlying (OvCa) cells dictating their lipid metabolic activities promoting tumor progression remain elusive. Here, we report that two critical fatty acid desaturases, stearoyl-CoA desaturase-1 (SCD1) acyl-CoA 6-desaturase (FADS2), are aberrantly upregulated, accelerating aggressiveness...
Abstract The Sox family of transcription factors plays a crucial role in the development vertebrate nervous system. In zebrafish embryo, sox1 genes are expressed neural progenitor cells and neurons ventral spinal cord. We recently reported that loss function sox1a sox1b leads to significant decline subtype V2 neurons, called V2s, zebrafish. Here, single-cell RNA sequencing approach was used analyse transcriptome lineage progenitors cord at four different time points during first five days...
The Sox family of transcription factors plays a crucial role in the development vertebrate nervous system. In zebrafish embryo, sox1 genes are expressed neural progenitor cells and neurons ventral spinal cord. We recently reported that loss function sox1a sox1b leads to significant decline subtype V2 neurons, called V2s, zebrafish. Here, single-cell RNA sequencing approach was used analyse transcriptome lineage progenitors cord at four different time points during first five days embryonic...
Abstract Tumor-associated macrophages (TAMs) are crucially associated with tumor development and progression; however, it remains unclear how the microenvironment (TME) rewires metabolic circuits preferentially induces TAMs to polarize toward a protumoral phenotype. Here, we report that polyunsaturated fatty acids (PUFAs) in malignant ascites promote M2-like deposition facilitate peritoneal metastases of epithelial ovarian cancer (EOC). We demonstrated PUFAs lipid-enriched inactivate RhoA,...