A5053775513- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Complement system in diseases
- interferon and immune responses
- vaccines and immunoinformatics approaches
- Dietary Effects on Health
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Virus-based gene therapy research
- Advanced Biosensing Techniques and Applications
- Immune cells in cancer
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Platelet Disorders and Treatments
- Acute Myeloid Leukemia Research
- Neuropeptides and Animal Physiology
- Distributed and Parallel Computing Systems
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
National Institute of Allergy and Infectious Diseases
2024-2025
National Institutes of Health
2025
Immune Regulation (United Kingdom)
2025
Albert Einstein College of Medicine
2020-2024
MRC Laboratory of Molecular Biology
2015
Abstract Acute myeloid leukemia (AML) is initiated and sustained by a hierarchy of stem cells (LSCs), elimination this cell population required for curative therapies. Here we show that transmembrane immunoglobulin domain containing 2 (TMIGD2), recently discovered co-stimulatory immune receptor, aberrantly expressed human AML cells, can be used to identify enrich functional LSCs. We demonstrate TMIGD2 the development maintenance self-renewal LSCs but not essential normal hematopoiesis....
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Using intravital imaging, we report that bone marrow (BM) plasma cells (PCs) are motile. BM PCs exhibit a unique migration pattern, characterized by intermittent periods of high motility and longer stretches confined or arrest. accumulate into clusters, which have reduced cell motility. APRIL promotes cluster formation overall PC in the BM. Although CXCL12 its receptor, CXCR4, promote BM, VLA4 activity However, blocking either pathway egress from Under steady-state conditions, recirculate to...
Multiplexed imaging is a powerful approach in spatial biology, although it complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, central resource for reagents, protocols more, to enhance knowledge sharing, optimization innovation of proteomics techniques.
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
T follicular helper (TFH) cells promote expansion of germinal center (GC) B and plasma cell differentiation. Whether cognate peptide-MHCII (pMHCII) density instructs selection fate decisions in a quantitative manner remains unclear. Using αDEC205-OVA to differentially deliver OVA peptides GC on the basis DEC205 allelic copy number, we find DEC205+/+ take up 2-fold more antigen than DEC205+/− cells, leading proportional TFH help expansion. To validate these results, establish caged peptide,...
Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intravital two-photon imaging, we find in contrast to most (PCs) bone marrow (BM), LLPCs are uniquely sessile organized into clusters APRIL, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome phenotype compared...
Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intravital two-photon imaging, we find in contrast to most (PCs) bone marrow (BM), LLPCs are uniquely sessile organized into clusters APRIL, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome phenotype compared...
Plasmacytoid dendritic cells (pDCs) are the most potent producer of type I interferon (IFN), but how pDC is primed in vivo poorly defined. Using a mouse model severe malaria, we have previously established that upon priming by CD169 + macrophages (MPs), initiates IFN-I secretion bone marrow (BM) infected mice via cell-intrinsic TLR7 sensing and cell-extrinsic STING sensing. Herein show MP both required for arrest during priming, suggesting source ligands. We establish chemotaxis functional...
Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intra-vital two-photon imaging, we find in contrast to most bone marrow (BM), LLPCs are uniquely sessile organized into clusters APRIL, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome phenotype compared PCs,...
The goal of this protocol is to evaluate the optimal snap (flash) freezing method for multiplexed tissue imaging. We detail four different methods (see sample preparation) preparing frozen tissues. additionally compare image quality, ease use, and safety each our preferred preservation (fixed with sucrose cryopreservation). To directly methods, tissues were collected from one mouse divided among five groups. determined that cryogenic Seal'N Freeze Box followed by post-fixation sections using...
Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intra-vital two-photon imaging, we find in contrast to most bone marrow, LLPCs are uniquely sessile organized into clusters April, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome proteome compared PCs, fine...
Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intra-vital two-photon imaging, we find in contrast to most bone marrow, LLPCs are uniquely sessile organized into clusters April, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome proteome compared PCs, fine...