A5008775427- Inflammasome and immune disorders
- Immune Cell Function and Interaction
- Vibrio bacteria research studies
- Immunotherapy and Immune Responses
- Burkholderia infections and melioidosis
- Microbial Metabolism and Applications
- Immune cells in cancer
- Immune responses and vaccinations
- CAR-T cell therapy research
- Yersinia bacterium, plague, ectoparasites research
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- Cell death mechanisms and regulation
- Autoimmune and Inflammatory Disorders Research
- interferon and immune responses
- Botanical Research and Chemistry
- T-cell and B-cell Immunology
- Genetics, Bioinformatics, and Biomedical Research
- Cell Image Analysis Techniques
- Radiomics and Machine Learning in Medical Imaging
- AI in cancer detection
- Insect and Pesticide Research
- Pancreatic and Hepatic Oncology Research
- Cancer, Stress, Anesthesia, and Immune Response
- Single-cell and spatial transcriptomics
University of California, San Diego
2025
University of North Carolina at Chapel Hill
2013-2023
National Institute of Allergy and Infectious Diseases
2021
National Institutes of Health
2021
National Institute of General Medical Sciences
2021
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary...
Either caspase-1 or caspase-11 can cleave gasdermin D to cause pyroptosis, eliminating intracellular replication niches. We previously showed that macrophages detect Burkholderia thailandensis via NLRC4, triggering the release of interleukin (IL)-18 and driving an essential interferon (IFN)-γ response primes caspase-11. now identify IFN-γ-producing cells as a mixture natural killer (NK) T cells. Although both in neutrophils, we find NLRC4-activated triggers pyroptosis macrophages, but this...
Abstract Granulomas often form around pathogens that cause chronic infections. Here, we discover an innate granuloma model in mice with environmental bacterium called Chromobacterium violaceum . Granuloma formation not only successfully walls off, but also clears, the infection. The infected lesion can arise from a single replicates despite presence of neutrophil swarm. Bacterial replication ceases when macrophages organize infection and granuloma. This response is accomplished independently...
Multiplexed imaging is a powerful approach in spatial biology, although it complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, central resource for reagents, protocols more, to enhance knowledge sharing, optimization innovation of proteomics techniques.
The highly pathogenic Yersinia enterocolitica strains have a chromosomally encoded type III secretion system (T3SS) that is expressed and functional in vitro only when the bacteria are cultured at 26 °C. Mutations render this nonfunctional slightly attenuated mouse model of infection following an oral inoculation early time points postinfection. discrepancy between temperature required for Ysa gene expression physiological mammalian systems has made defining role T3SS challenging. Therefore,...
Granulomas often form around pathogens that cause chronic infections. Here, we discover a novel granuloma model in mice.
Abstract Recent success in the use of immunotherapy for a broad range cancers has propelled field cancer immunology to forefront research. As more and young investigators join community immunologists, Arthur L. Irving Family Foundation Cancer Immunology Symposium provided platform bring this expanding vibrant together support development future leaders field. This commentary outlines lessons that emerged from inaugural symposium highlighting areas scientific career are essential professional...
Abstract Our lab has shown that inflammasome-triggered pyroptosis ejects microbes from replication niches in macrophages, exposing them to neutrophil killing. Because bona fide intracellular pathogens must evade vivo, the role of defense primarily been observed deprived evasion strategies. To identify naturally occurring bacteria are cleared via pyroptosis, we considered specific chronic granulomatous disease (CGD), a killing deficiency. We hypothesize subset these may be detected by...
Abstract Despite major advances in immune checkpoint blockade therapies, tumor therapeutic responsiveness remains difficult to predict. This is especially true for pancreatic ductal adenocarcinoma, where total T cell numbers the proper do not predict responses blockade. Here, we use a novel congenic KPC mouse model-derived clone library that recapitulates spectrum of heterogeneity seen patients, enabling us perform head-to-head comparisons between therapeutically responsive and refractory...
<h3>Background</h3> Pancreatic ductal adenocarcinoma (PDA) is an aggressive and insidious cancer because it often remains undetectable until later stages typically refractory to current treatments. Additionally, the tumor microenvironment (TME) of PDA tumors immunologically heterogeneous, which complicates therapies like checkpoint blockade. Many mouse models fail recapitulate heterogeneity seen in human cancer, something that has severely constrained our understanding mechanistic response...