A5021526460- Peroxisome Proliferator-Activated Receptors
- Metabolism and Genetic Disorders
- Smoking Behavior and Cessation
- RNA modifications and cancer
- Biochemical Analysis and Sensing Techniques
- Amino Acid Enzymes and Metabolism
- Fermentation and Sensory Analysis
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Advanced Chemical Sensor Technologies
- Fungal and yeast genetics research
- Consumer Attitudes and Food Labeling
- Olfactory and Sensory Function Studies
- Drug Transport and Resistance Mechanisms
- Epigenetics and DNA Methylation
- Antifungal resistance and susceptibility
- Adrenal Hormones and Disorders
- Eosinophilic Disorders and Syndromes
- Dialysis and Renal Disease Management
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Yeasts and Rust Fungi Studies
- RNA and protein synthesis mechanisms
- Systemic Lupus Erythematosus Research
- Bone and Joint Diseases
National Institute for Public Health and the Environment
2016-2024
Amsterdam University Medical Centers
2021-2022
Wilhelmina Children's Hospital
2011-2015
University Medical Center Utrecht
2011-2015
Amsterdam UMC Location University of Amsterdam
2008-2014
University of Amsterdam
2002-2014
Utrecht University
1995-2013
Netherlands Metabolomics Centre
2011-2012
University Medical Center
2012
University Hospital and Clinics
2012
Type species of 75 yeast genera were examined for their ability to grow anaerobically in complex and mineral media. To define anaerobic conditions, we added a redox indicator, resazurin, the media determine low potentials. All strains tested capable fermenting glucose ethanol oxygen-limited shake-flask cultures, even those generally regarded as nonfermentative. However, only 23% grew under conditions. A comparative study with number selected revealed that Saccharomyces cerevisiae stands out...
Peroxisomes play a major role in human cellular lipid metabolism, including the beta-oxidation of fatty acids. The most frequent peroxisomal disorder is X-linked adrenoleukodystrophy (X-ALD), which caused by mutations ABCD1 gene. protein involved, called ABCD1, or alternatively ALDP, member ATP-binding-cassette (ABC) transporter family and located membrane. biochemical hallmark X-ALD accumulation very long-chain acids (VLCFAs), due to an impaired beta-oxidation. Although this suggests ALDP...
ABCD3 is one of three ATP-binding cassette (ABC) transporters present in the peroxisomal membrane catalyzing ATP-dependent transport substrates for metabolic pathways localized peroxisomes. So far, precise function not known. Here, we report identification first patient with a defect ABCD3. The presented hepatosplenomegaly and severe liver disease showed striking accumulation C27-bile acid intermediates plasma. Investigation parameters skin fibroblasts revealed reduced number enlarged...
This works aimed to assess the health risks of e-cigarette use bystanders. The exhaled breath 17 volunteers was collected while they were vaping, and levels nicotine, propylene glycol, glycerol, formaldehyde, acetaldehyde, acrolein, tobacco-specific nitrosamines (TSNAs), heavy metals analyzed. Increased TSNAs copper found in volunteers. From these measurements, bystander exposure estimated for two different scenarios: (1) A non-ventilated car with users (2) a ventilated office one user. Our...
X-linked adrenoleukodystrophy (ALD) is a devastating metabolic disorder affecting the adrenal glands, brain and spinal cord. Males with ALD are at high risk for developing insufficiency or progressive cerebral white matter lesions (cerebral ALD) an early age. If untreated, often fatal. Women not ALD. Newborn screening in males enables prospective monitoring timely therapeutic intervention, thereby preventing irreparable damage saving lives. The Dutch Ministry of Health adopted advice Council...
Growth and metabolite formation were studied in oxygen-limited chemostat cultures of Saccharomyces cerevisiae CBS 8066 Candida utilis 621 growing on glucose or maltose at a dilution rate 0·1 h−1 With either S. could be grown under dual limitation oxygen sugar. Respiration alcoholic fermentation occurred simultaneously the catabolite fluxes through these processes dependent magnitude feed. C. also oxygen. However, very low feed rates (i.e. below 4 mmol l−1 h−1) growth was limited by only, as...
The aminoglycoside kasugamycin, which has previously been shown to inhibit initiation of protein biosynthesis in vitro, also affects translational accuracy vitro. This is deduced from the observation that drug decreases incorporation histidine relative alanine into coat phage MS2, gene devoid codons. read-through MS2 cistron, due frameshifts suppressed by antibiotic. In contrast, streptomycin enhances and this system. effects kasugamycin take place at concentrations do not biosynthesis....
Objectives Recent years have seen an increase in e-liquids containing nicotine salts. Nicotine salts are less harsh and bitter than free-base therefore can facilitate inhalation. Because inhalation-facilitating ingredients banned the European Union, we assessed occurrence characteristics of salt-containing notified for Netherlands. Methods We analysed data 39 030 products, submitted by manufacturers Union Common Entry Gate system, as extracted on 30 June 2020. Results were present 13%...
Objectives This study combines chemical analysis and flavour descriptions of additives used in tobacco products, provides a starting point to build an extensive library components, useful for product surveillance. Methods Headspace gas chromatography-mass spectrometry (GC-MS) was compare 22 commercially available products (cigarettes roll-your-own) expected have characterising 6 not with 5 reference (natural leaves research cigarettes containing no additives). The components naturally...
Males with X-linked adrenoleukodystrophy (ALD) are at high risk for developing adrenal insufficiency and/or progressive leukodystrophy (cerebral ALD) an early age. Pathogenic variants in ABCD1 result elevated levels of very long-chain fatty acids (VLCFA), including C26:0-lysophosphatidylcholine (C26:0-LPC). Newborn screening ALD enables prospective monitoring and timely therapeutic intervention, thereby preventing irreversible damage saving lives. The Dutch Health Council recommended to...
Background Vitamin B6 is present in various forms (vitamers) the diet that need to be metabolized pyridoxal phosphate (PLP), active cofactor form of vitamin B6. In literature, liver has been reported major site for this conversion, whereas exact role intestine remains elucidated. Objective To gain insight into human metabolism. Materials and Methods Expression enzymes kinase (PK), pyridox(am)ine oxidase (PNPO) PLP-phosphatase was determined Caco-2 cells lysates intestine. uptake, conversion...
In the US, nicotine salts (with protonated instead of free-based nicotine) have been reported to lower harshness and bitterness e-cigarette aerosols, making it easier inhale high levels nicotine. This study aimed determine whether also increase sensory appeal at concentrations (< 20 mg/mL). Moreover, novel, inhalation intensity both types e-liquids was compared. a randomized, double-blinded, within-participants design, healthy adults who use e-cigarettes (n=68) vaped tobacco-flavored...
In eukaryotes, acetyl coenzyme A (acetyl-CoA) produced during peroxisomal fatty acid beta-oxidation needs to be transported mitochondria for further metabolism. Two parallel pathways acetyl-CoA transport have been identified in Saccharomyces cerevisiae; one is dependent on citrate synthase (Cit), while the other requires and mitochondrial carnitine acetyltransferase (Cat) activities. Here we show that human fungal pathogen Candida albicans lacks Cit, relying exclusively Cat activity of...
The pentose phosphate pathway (PPP) is the main source of NADPH in cell and therefore essential for maintenance redox balance anabolic reactions. produced by two dehydrogenases oxidative branch PPP: glucose-6-phosphate dehydrogenase (Zwf1) 6-phosphogluconate (Gnd1). We observed that commensal fungus Candida albicans these enzymes contain putative peroxisomal targeting signals (PTSs): Zwf1 has a PTS1, while annotated intron GND1 encodes PTS2. By subcellular fractionation fluorescence...
A family of eukaryotic proline racemase-like genes has recently been identified. Several members this have well characterized and are known to catalyze the racemization free or trans-4-hydroxyproline. However, majority proteins, including a human protein called C14orf149, lack specific cysteine residue that is be critical for racemase activity. Instead, these proteins invariably contain threonine at position. The function enzymes remained unresolved until now. In study, we demonstrate three...
The possible physiological role of mitochondria in anaerobically grown Saccharomyces cerevisiaewas investigated via enzyme localization and inhibitor studies. Almost all the activity citrate synthase (EC 4.1.3.7) was recovered mitochondrial fraction after differential centrifugation spheroplast lysates. exhibited a high degree latency which demonstrated by sonication fractions. Since is an important anabolic reactions, consequence this requirement for transport metabolites across membranes....
Objective Electronic cigarettes are addictive and harmful, flavour is a key factor determining their abuse liability. Both adult smokers young non-smokers like sweet fruity flavours in particular. In order to discourage e-cigarette use among youth, the Dutch government announced 2020 only allow tobacco e-liquids. We propose restrictive list of flavourings that will enable production e-liquids with flavour. Methods used e-liquid ingredient data notified via European Common Entry Gate system...